Cargando…

3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method

Bio-inks consisting of pectin (Pec), carboxymethyl cellulose (CMC), and ZnO nanoparticles (ZnO) were used to prepare films by solution casting and 3D-printing methods. Field emission scanning electron microscopy (FE-SEM) was conducted to observe that the surface of samples made by 3D bioprinter was...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Yeon Ho, Priyadarshi, Ruchir, Kim, Jin-Wook, Kim, Jangwhan, Alekseev, Denis G., Rhim, Jong-Whan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657909/
https://www.ncbi.nlm.nih.gov/pubmed/36365704
http://dx.doi.org/10.3390/polym14214711
_version_ 1784829814436265984
author Kim, Yeon Ho
Priyadarshi, Ruchir
Kim, Jin-Wook
Kim, Jangwhan
Alekseev, Denis G.
Rhim, Jong-Whan
author_facet Kim, Yeon Ho
Priyadarshi, Ruchir
Kim, Jin-Wook
Kim, Jangwhan
Alekseev, Denis G.
Rhim, Jong-Whan
author_sort Kim, Yeon Ho
collection PubMed
description Bio-inks consisting of pectin (Pec), carboxymethyl cellulose (CMC), and ZnO nanoparticles (ZnO) were used to prepare films by solution casting and 3D-printing methods. Field emission scanning electron microscopy (FE-SEM) was conducted to observe that the surface of samples made by 3D bioprinter was denser and more compact than the solution cast samples. In addition, Pec/CMC/ZnO made by 3D-bioprinter (Pec/CMC/ZnO-3D) revealed enhanced water vapor barrier, hydrophobicity, and mechanical properties. Pec/CMC/ZnO-3D also showed strong antimicrobial activity within 12 h against S. aureus and E. coli O157: H7 bacterial strains compared to the solution cast films. Further, the nanocomposite bio-inks used for 3D printing did not show cytotoxicity towards normal human dermal fibroblast (NDFB) cells but enhanced the fibroblast proliferation with increasing exposure concentration of the sample. The study provided two important inferences. Firstly, the 3D bioprinting method can be an alternative, better, and more practical method for fabricating biopolymer film instead of solution casting, which is the main finding of this work defining its novelty. Secondly, the Pec/CMC/ZnO can potentially be used as 3D bio-inks to fabricate functional films or scaffolds and biomedical applications.
format Online
Article
Text
id pubmed-9657909
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96579092022-11-15 3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method Kim, Yeon Ho Priyadarshi, Ruchir Kim, Jin-Wook Kim, Jangwhan Alekseev, Denis G. Rhim, Jong-Whan Polymers (Basel) Article Bio-inks consisting of pectin (Pec), carboxymethyl cellulose (CMC), and ZnO nanoparticles (ZnO) were used to prepare films by solution casting and 3D-printing methods. Field emission scanning electron microscopy (FE-SEM) was conducted to observe that the surface of samples made by 3D bioprinter was denser and more compact than the solution cast samples. In addition, Pec/CMC/ZnO made by 3D-bioprinter (Pec/CMC/ZnO-3D) revealed enhanced water vapor barrier, hydrophobicity, and mechanical properties. Pec/CMC/ZnO-3D also showed strong antimicrobial activity within 12 h against S. aureus and E. coli O157: H7 bacterial strains compared to the solution cast films. Further, the nanocomposite bio-inks used for 3D printing did not show cytotoxicity towards normal human dermal fibroblast (NDFB) cells but enhanced the fibroblast proliferation with increasing exposure concentration of the sample. The study provided two important inferences. Firstly, the 3D bioprinting method can be an alternative, better, and more practical method for fabricating biopolymer film instead of solution casting, which is the main finding of this work defining its novelty. Secondly, the Pec/CMC/ZnO can potentially be used as 3D bio-inks to fabricate functional films or scaffolds and biomedical applications. MDPI 2022-11-03 /pmc/articles/PMC9657909/ /pubmed/36365704 http://dx.doi.org/10.3390/polym14214711 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yeon Ho
Priyadarshi, Ruchir
Kim, Jin-Wook
Kim, Jangwhan
Alekseev, Denis G.
Rhim, Jong-Whan
3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
title 3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
title_full 3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
title_fullStr 3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
title_full_unstemmed 3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
title_short 3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
title_sort 3d-printed pectin/carboxymethyl cellulose/zno bio-inks: comparative analysis with the solution casting method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657909/
https://www.ncbi.nlm.nih.gov/pubmed/36365704
http://dx.doi.org/10.3390/polym14214711
work_keys_str_mv AT kimyeonho 3dprintedpectincarboxymethylcelluloseznobioinkscomparativeanalysiswiththesolutioncastingmethod
AT priyadarshiruchir 3dprintedpectincarboxymethylcelluloseznobioinkscomparativeanalysiswiththesolutioncastingmethod
AT kimjinwook 3dprintedpectincarboxymethylcelluloseznobioinkscomparativeanalysiswiththesolutioncastingmethod
AT kimjangwhan 3dprintedpectincarboxymethylcelluloseznobioinkscomparativeanalysiswiththesolutioncastingmethod
AT alekseevdenisg 3dprintedpectincarboxymethylcelluloseznobioinkscomparativeanalysiswiththesolutioncastingmethod
AT rhimjongwhan 3dprintedpectincarboxymethylcelluloseznobioinkscomparativeanalysiswiththesolutioncastingmethod