Cargando…
Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs
Cerebrovascular disease is one of the leading causes of disability and death worldwide, and seeking a potential treatment is essential. Trilinolein (TriL) is a natural triacylglycerol presented in several plants. The effects of TriL on cerebrovascular diseases such as cerebral ischemia and carotid s...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658252/ https://www.ncbi.nlm.nih.gov/pubmed/36361610 http://dx.doi.org/10.3390/ijms232112820 |
_version_ | 1784829904827711488 |
---|---|
author | Chen, Yuh-Fung Wu, Kuo-Jen Siao, Lian-Ru Tsai, Huei-Yann |
author_facet | Chen, Yuh-Fung Wu, Kuo-Jen Siao, Lian-Ru Tsai, Huei-Yann |
author_sort | Chen, Yuh-Fung |
collection | PubMed |
description | Cerebrovascular disease is one of the leading causes of disability and death worldwide, and seeking a potential treatment is essential. Trilinolein (TriL) is a natural triacylglycerol presented in several plants. The effects of TriL on cerebrovascular diseases such as cerebral ischemia and carotid stenosis have never been studied. Accordingly, we investigated the protection of TriL on cerebral ischemia/reperfusion (I/R) and vascular smooth muscle cell (VSMC) migration in vivo and in vitro. The cerebral infarction area, the intima to media area (I/M ratio), and proliferating cell nuclear antigen (PCNA)-staining of the carotid artery were measured. Platelet-derived growth factor (PDGF)-BB-stimulated A7r5 cell migration and potential mechanisms of TriL were investigated by wound healing, transwell, and Western blotting. TriL (50, 100, and 200 mg/kg, p.o.) reduced: the cerebral infarction area; neurological deficit; TUNEL-positive apoptosis; intimal hyperplasia; and PCNA-positive cells in rodents. TriL (5, 10, and 20 µM) significantly inhibited PDGF-BB-stimulated A7r5 cell migration and reduced matrix metalloproteinase-2 (MMP-2), Ras, MEK, and p-ERK protein levels in PDGF-BB-stimulated A7r5 cells. TriL is protective in models of I/R-induced brain injury, carotid artery ligation-induced intimal hyperplasia, and VSMC migration both in vivo and in vitro. TriL could be potentially efficacious in preventing cerebral ischemia and cerebrovascular diseases. |
format | Online Article Text |
id | pubmed-9658252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96582522022-11-15 Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs Chen, Yuh-Fung Wu, Kuo-Jen Siao, Lian-Ru Tsai, Huei-Yann Int J Mol Sci Article Cerebrovascular disease is one of the leading causes of disability and death worldwide, and seeking a potential treatment is essential. Trilinolein (TriL) is a natural triacylglycerol presented in several plants. The effects of TriL on cerebrovascular diseases such as cerebral ischemia and carotid stenosis have never been studied. Accordingly, we investigated the protection of TriL on cerebral ischemia/reperfusion (I/R) and vascular smooth muscle cell (VSMC) migration in vivo and in vitro. The cerebral infarction area, the intima to media area (I/M ratio), and proliferating cell nuclear antigen (PCNA)-staining of the carotid artery were measured. Platelet-derived growth factor (PDGF)-BB-stimulated A7r5 cell migration and potential mechanisms of TriL were investigated by wound healing, transwell, and Western blotting. TriL (50, 100, and 200 mg/kg, p.o.) reduced: the cerebral infarction area; neurological deficit; TUNEL-positive apoptosis; intimal hyperplasia; and PCNA-positive cells in rodents. TriL (5, 10, and 20 µM) significantly inhibited PDGF-BB-stimulated A7r5 cell migration and reduced matrix metalloproteinase-2 (MMP-2), Ras, MEK, and p-ERK protein levels in PDGF-BB-stimulated A7r5 cells. TriL is protective in models of I/R-induced brain injury, carotid artery ligation-induced intimal hyperplasia, and VSMC migration both in vivo and in vitro. TriL could be potentially efficacious in preventing cerebral ischemia and cerebrovascular diseases. MDPI 2022-10-24 /pmc/articles/PMC9658252/ /pubmed/36361610 http://dx.doi.org/10.3390/ijms232112820 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yuh-Fung Wu, Kuo-Jen Siao, Lian-Ru Tsai, Huei-Yann Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs |
title | Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs |
title_full | Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs |
title_fullStr | Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs |
title_full_unstemmed | Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs |
title_short | Trilinolein, a Natural Triacylglycerol, Protects Cerebral Ischemia through Inhibition of Neuronal Apoptosis and Ameliorates Intimal Hyperplasia via Attenuation of Migration and Modulation of Matrix Metalloproteinase-2 and RAS/MEK/ERK Signaling Pathway in VSMCs |
title_sort | trilinolein, a natural triacylglycerol, protects cerebral ischemia through inhibition of neuronal apoptosis and ameliorates intimal hyperplasia via attenuation of migration and modulation of matrix metalloproteinase-2 and ras/mek/erk signaling pathway in vsmcs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658252/ https://www.ncbi.nlm.nih.gov/pubmed/36361610 http://dx.doi.org/10.3390/ijms232112820 |
work_keys_str_mv | AT chenyuhfung trilinoleinanaturaltriacylglycerolprotectscerebralischemiathroughinhibitionofneuronalapoptosisandamelioratesintimalhyperplasiaviaattenuationofmigrationandmodulationofmatrixmetalloproteinase2andrasmekerksignalingpathwayinvsmcs AT wukuojen trilinoleinanaturaltriacylglycerolprotectscerebralischemiathroughinhibitionofneuronalapoptosisandamelioratesintimalhyperplasiaviaattenuationofmigrationandmodulationofmatrixmetalloproteinase2andrasmekerksignalingpathwayinvsmcs AT siaolianru trilinoleinanaturaltriacylglycerolprotectscerebralischemiathroughinhibitionofneuronalapoptosisandamelioratesintimalhyperplasiaviaattenuationofmigrationandmodulationofmatrixmetalloproteinase2andrasmekerksignalingpathwayinvsmcs AT tsaihueiyann trilinoleinanaturaltriacylglycerolprotectscerebralischemiathroughinhibitionofneuronalapoptosisandamelioratesintimalhyperplasiaviaattenuationofmigrationandmodulationofmatrixmetalloproteinase2andrasmekerksignalingpathwayinvsmcs |