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Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee
The production and consumption of organic fresh produce have constantly increased since the 1990s. Consumers prefer organic produce because it does not contain synthetic chemical residues that are often implicated in health problems. The contamination of fresh produce by pathogenic Enterobacteriacea...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658351/ https://www.ncbi.nlm.nih.gov/pubmed/36359929 http://dx.doi.org/10.3390/foods11213316 |
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author | Akaeze, Onyekachukwu Kilonzo-Nthenge, Agnes Nandwani, Dilip Mafiz, Abdullah Ibn Nzomo, Maureen Aniume, Tobenna |
author_facet | Akaeze, Onyekachukwu Kilonzo-Nthenge, Agnes Nandwani, Dilip Mafiz, Abdullah Ibn Nzomo, Maureen Aniume, Tobenna |
author_sort | Akaeze, Onyekachukwu |
collection | PubMed |
description | The production and consumption of organic fresh produce have constantly increased since the 1990s. Consumers prefer organic produce because it does not contain synthetic chemical residues that are often implicated in health problems. The contamination of fresh produce by pathogenic Enterobacteriaceae strains remains a major challenge, and is responsible for frequent foodborne disease outbreaks. The use of antibiotics has proved an effective treatment, but the increase in occurrences of antibiotic resistance is becoming a health challenge. This study seeks to establish the presence of antimicrobial resistance in Enterobacteriaceae on organic and conventional watermelon fruits. Watermelons used for this study were cultivated at the Tennessee State University Certified Organic Farm, Nashville. At harvest, nine fruits were selected from among fruits lying on plastic mulch, and nine from fruits lying on the soil of both organic and conventional plots. These were placed in sterile sample bags for microbial analysis. Spread plating technique, API 20E, and apiweb software were used for microbial isolation and identification. Identified strains were tested for antimicrobial resistance against 12 common antibiotics. Seventeen Enterobacteriaceae strains were isolated and identified. Isolates were susceptible to gentamycin, ciprofloxacin, and chloramphenicol, but were resistant to cefoxitin. Citrobacter freundii showed a 14.3% resistance to Streptomycin. Pantoea spp. and Providencia rettigeri showed 50% and 100% resistance to tetracycline. Findings from this study confirm the presence of antibiotic-resistant Enterobacteriaceae strains on organic watermelons in Nashville, TN. |
format | Online Article Text |
id | pubmed-9658351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96583512022-11-15 Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee Akaeze, Onyekachukwu Kilonzo-Nthenge, Agnes Nandwani, Dilip Mafiz, Abdullah Ibn Nzomo, Maureen Aniume, Tobenna Foods Communication The production and consumption of organic fresh produce have constantly increased since the 1990s. Consumers prefer organic produce because it does not contain synthetic chemical residues that are often implicated in health problems. The contamination of fresh produce by pathogenic Enterobacteriaceae strains remains a major challenge, and is responsible for frequent foodborne disease outbreaks. The use of antibiotics has proved an effective treatment, but the increase in occurrences of antibiotic resistance is becoming a health challenge. This study seeks to establish the presence of antimicrobial resistance in Enterobacteriaceae on organic and conventional watermelon fruits. Watermelons used for this study were cultivated at the Tennessee State University Certified Organic Farm, Nashville. At harvest, nine fruits were selected from among fruits lying on plastic mulch, and nine from fruits lying on the soil of both organic and conventional plots. These were placed in sterile sample bags for microbial analysis. Spread plating technique, API 20E, and apiweb software were used for microbial isolation and identification. Identified strains were tested for antimicrobial resistance against 12 common antibiotics. Seventeen Enterobacteriaceae strains were isolated and identified. Isolates were susceptible to gentamycin, ciprofloxacin, and chloramphenicol, but were resistant to cefoxitin. Citrobacter freundii showed a 14.3% resistance to Streptomycin. Pantoea spp. and Providencia rettigeri showed 50% and 100% resistance to tetracycline. Findings from this study confirm the presence of antibiotic-resistant Enterobacteriaceae strains on organic watermelons in Nashville, TN. MDPI 2022-10-22 /pmc/articles/PMC9658351/ /pubmed/36359929 http://dx.doi.org/10.3390/foods11213316 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Akaeze, Onyekachukwu Kilonzo-Nthenge, Agnes Nandwani, Dilip Mafiz, Abdullah Ibn Nzomo, Maureen Aniume, Tobenna Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee |
title | Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee |
title_full | Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee |
title_fullStr | Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee |
title_full_unstemmed | Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee |
title_short | Incidence of Drug-Resistant Enterobacteriaceae Strains in Organic and Conventional Watermelons Grown in Tennessee |
title_sort | incidence of drug-resistant enterobacteriaceae strains in organic and conventional watermelons grown in tennessee |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658351/ https://www.ncbi.nlm.nih.gov/pubmed/36359929 http://dx.doi.org/10.3390/foods11213316 |
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