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The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images

Staphylococcal biofilms are major causative factors of non-healing wound infections. Their treatment algorithms recommend the use of locally applied antiseptic agents to counteract the spread of infection. The efficacy of antiseptics against biofilm is assessed in vitro by a set of standard quantita...

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Autores principales: Krasowski, Grzegorz, Migdał, Paweł, Woroszyło, Marta, Fijałkowski, Karol, Chodaczek, Grzegorz, Czajkowska, Joanna, Dudek, Bartłomiej, Nowicka, Joanna, Oleksy-Wawrzyniak, Monika, Kwiek, Bartłomiej, Paleczny, Justyna, Brożyna, Malwina, Junka, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658380/
https://www.ncbi.nlm.nih.gov/pubmed/36362310
http://dx.doi.org/10.3390/ijms232113524
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author Krasowski, Grzegorz
Migdał, Paweł
Woroszyło, Marta
Fijałkowski, Karol
Chodaczek, Grzegorz
Czajkowska, Joanna
Dudek, Bartłomiej
Nowicka, Joanna
Oleksy-Wawrzyniak, Monika
Kwiek, Bartłomiej
Paleczny, Justyna
Brożyna, Malwina
Junka, Adam
author_facet Krasowski, Grzegorz
Migdał, Paweł
Woroszyło, Marta
Fijałkowski, Karol
Chodaczek, Grzegorz
Czajkowska, Joanna
Dudek, Bartłomiej
Nowicka, Joanna
Oleksy-Wawrzyniak, Monika
Kwiek, Bartłomiej
Paleczny, Justyna
Brożyna, Malwina
Junka, Adam
author_sort Krasowski, Grzegorz
collection PubMed
description Staphylococcal biofilms are major causative factors of non-healing wound infections. Their treatment algorithms recommend the use of locally applied antiseptic agents to counteract the spread of infection. The efficacy of antiseptics against biofilm is assessed in vitro by a set of standard quantitative and semi-quantitative methods. The development of software for image processing additionally allowed for the obtainment of quantitative data from microscopic images of biofilm dyed with propidium iodine and SYTO-9 reagents, differentiating dead cells from live ones. In this work, the method of assessment of the impact of antiseptic agents on staphylococcal biofilm in vitro, based on biofilms’ processed images, was proposed and scrutinized with regard to clinically relevant antiseptics, polyhexanide, povidone–iodine and hypochlorite. The standard quantitative culturing method was applied to validate the obtained data from processed images. The results indicated significantly higher activity of polyhexanide and povidone–iodine than hypochlorite against staphylococcal biofilm. Taking into account the fact that in vitro results of the efficacy of antiseptic agents against staphylococcal biofilm are frequently applied to back up their use in hospitals and ambulatory units, our work should be considered an important tool; providing reliable, quantitative data in this regard.
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spelling pubmed-96583802022-11-15 The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images Krasowski, Grzegorz Migdał, Paweł Woroszyło, Marta Fijałkowski, Karol Chodaczek, Grzegorz Czajkowska, Joanna Dudek, Bartłomiej Nowicka, Joanna Oleksy-Wawrzyniak, Monika Kwiek, Bartłomiej Paleczny, Justyna Brożyna, Malwina Junka, Adam Int J Mol Sci Article Staphylococcal biofilms are major causative factors of non-healing wound infections. Their treatment algorithms recommend the use of locally applied antiseptic agents to counteract the spread of infection. The efficacy of antiseptics against biofilm is assessed in vitro by a set of standard quantitative and semi-quantitative methods. The development of software for image processing additionally allowed for the obtainment of quantitative data from microscopic images of biofilm dyed with propidium iodine and SYTO-9 reagents, differentiating dead cells from live ones. In this work, the method of assessment of the impact of antiseptic agents on staphylococcal biofilm in vitro, based on biofilms’ processed images, was proposed and scrutinized with regard to clinically relevant antiseptics, polyhexanide, povidone–iodine and hypochlorite. The standard quantitative culturing method was applied to validate the obtained data from processed images. The results indicated significantly higher activity of polyhexanide and povidone–iodine than hypochlorite against staphylococcal biofilm. Taking into account the fact that in vitro results of the efficacy of antiseptic agents against staphylococcal biofilm are frequently applied to back up their use in hospitals and ambulatory units, our work should be considered an important tool; providing reliable, quantitative data in this regard. MDPI 2022-11-04 /pmc/articles/PMC9658380/ /pubmed/36362310 http://dx.doi.org/10.3390/ijms232113524 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krasowski, Grzegorz
Migdał, Paweł
Woroszyło, Marta
Fijałkowski, Karol
Chodaczek, Grzegorz
Czajkowska, Joanna
Dudek, Bartłomiej
Nowicka, Joanna
Oleksy-Wawrzyniak, Monika
Kwiek, Bartłomiej
Paleczny, Justyna
Brożyna, Malwina
Junka, Adam
The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images
title The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images
title_full The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images
title_fullStr The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images
title_full_unstemmed The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images
title_short The Assessment of Activity of Antiseptic Agents against Biofilm of Staphylococcus aureus Measured with the Use of Processed Microscopic Images
title_sort assessment of activity of antiseptic agents against biofilm of staphylococcus aureus measured with the use of processed microscopic images
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658380/
https://www.ncbi.nlm.nih.gov/pubmed/36362310
http://dx.doi.org/10.3390/ijms232113524
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