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Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro

The human central nervous system (CNS) is separated from the blood by distinct cellular barriers, including the blood–brain barrier (BBB) and the blood–cerebrospinal fluid (CFS) barrier (BCSFB). Whereas at the center of the BBB are the endothelial cells of the brain capillaries, the BCSFB is formed...

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Autores principales: Banovic, Franjo, Schulze, Sandrin, Abu Mraheil, Mobarak, Hain, Torsten, Chakraborty, Trinad, Orian-Rousseau, Véronique, Moroniak, Selina, Weiss, Christel, Ishikawa, Hiroshi, Schroten, Horst, Adam, Rüdiger, Schwerk, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658511/
https://www.ncbi.nlm.nih.gov/pubmed/36361697
http://dx.doi.org/10.3390/ijms232112908
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author Banovic, Franjo
Schulze, Sandrin
Abu Mraheil, Mobarak
Hain, Torsten
Chakraborty, Trinad
Orian-Rousseau, Véronique
Moroniak, Selina
Weiss, Christel
Ishikawa, Hiroshi
Schroten, Horst
Adam, Rüdiger
Schwerk, Christian
author_facet Banovic, Franjo
Schulze, Sandrin
Abu Mraheil, Mobarak
Hain, Torsten
Chakraborty, Trinad
Orian-Rousseau, Véronique
Moroniak, Selina
Weiss, Christel
Ishikawa, Hiroshi
Schroten, Horst
Adam, Rüdiger
Schwerk, Christian
author_sort Banovic, Franjo
collection PubMed
description The human central nervous system (CNS) is separated from the blood by distinct cellular barriers, including the blood–brain barrier (BBB) and the blood–cerebrospinal fluid (CFS) barrier (BCSFB). Whereas at the center of the BBB are the endothelial cells of the brain capillaries, the BCSFB is formed by the epithelium of the choroid plexus. Invasion of cells of either the BBB or the BCSFB is a potential first step during CNS entry by the Gram-positive bacterium Listeria monocytogenes (Lm). Lm possesses several virulence factors mediating host cell entry, such as the internalin protein family—including internalin (InlA), which binds E-cadherin (Ecad) on the surface of target cells, and internalin B (InlB)—interacting with the host cell receptor tyrosine kinase Met. A further family member is internalin (InlF), which targets the intermediate filament protein vimentin. Whereas InlF has been shown to play a role during brain invasion at the BBB, its function during infection at the BCSFB is not known. We use human brain microvascular endothelial cells (HBMEC) and human choroid plexus epithelial papilloma (HIBCPP) cells to investigate the roles of InlF and vimentin during CNS invasion by Lm. Whereas HBMEC present intracellular and surface vimentin (besides Met), HIBCPP cells do not express vimentin (except Met and Ecad). Treatment with the surface vimentin modulator withaferin A (WitA) inhibited invasion of Lm into HBMEC, but not HIBCPP cells. Invasion of Lm into HBMEC and HIBCPP cells is, however, independent of InlF, since a deletion mutant of Lm lacking InlF did not display reduced invasion rates.
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spelling pubmed-96585112022-11-15 Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro Banovic, Franjo Schulze, Sandrin Abu Mraheil, Mobarak Hain, Torsten Chakraborty, Trinad Orian-Rousseau, Véronique Moroniak, Selina Weiss, Christel Ishikawa, Hiroshi Schroten, Horst Adam, Rüdiger Schwerk, Christian Int J Mol Sci Article The human central nervous system (CNS) is separated from the blood by distinct cellular barriers, including the blood–brain barrier (BBB) and the blood–cerebrospinal fluid (CFS) barrier (BCSFB). Whereas at the center of the BBB are the endothelial cells of the brain capillaries, the BCSFB is formed by the epithelium of the choroid plexus. Invasion of cells of either the BBB or the BCSFB is a potential first step during CNS entry by the Gram-positive bacterium Listeria monocytogenes (Lm). Lm possesses several virulence factors mediating host cell entry, such as the internalin protein family—including internalin (InlA), which binds E-cadherin (Ecad) on the surface of target cells, and internalin B (InlB)—interacting with the host cell receptor tyrosine kinase Met. A further family member is internalin (InlF), which targets the intermediate filament protein vimentin. Whereas InlF has been shown to play a role during brain invasion at the BBB, its function during infection at the BCSFB is not known. We use human brain microvascular endothelial cells (HBMEC) and human choroid plexus epithelial papilloma (HIBCPP) cells to investigate the roles of InlF and vimentin during CNS invasion by Lm. Whereas HBMEC present intracellular and surface vimentin (besides Met), HIBCPP cells do not express vimentin (except Met and Ecad). Treatment with the surface vimentin modulator withaferin A (WitA) inhibited invasion of Lm into HBMEC, but not HIBCPP cells. Invasion of Lm into HBMEC and HIBCPP cells is, however, independent of InlF, since a deletion mutant of Lm lacking InlF did not display reduced invasion rates. MDPI 2022-10-26 /pmc/articles/PMC9658511/ /pubmed/36361697 http://dx.doi.org/10.3390/ijms232112908 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Banovic, Franjo
Schulze, Sandrin
Abu Mraheil, Mobarak
Hain, Torsten
Chakraborty, Trinad
Orian-Rousseau, Véronique
Moroniak, Selina
Weiss, Christel
Ishikawa, Hiroshi
Schroten, Horst
Adam, Rüdiger
Schwerk, Christian
Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro
title Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro
title_full Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro
title_fullStr Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro
title_full_unstemmed Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro
title_short Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro
title_sort different involvement of vimentin during invasion by listeria monocytogenes at the blood–brain and the blood–cerebrospinal fluid barriers in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658511/
https://www.ncbi.nlm.nih.gov/pubmed/36361697
http://dx.doi.org/10.3390/ijms232112908
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