Cargando…

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

SIMPLE SUMMARY: The Shitou goose, the largest cultivated goose breed in China, has high research value in meat traits. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the mRNA and lncRNA expression profiles of Shitou goose myoblast in pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Jiahui, Zhang, Shuai, Chen, Genghua, Deng, Xianqi, Zhang, Danlu, Wen, Huaqiang, Yin, Yunqian, Lin, Zetong, Zhang, Xiquan, Luo, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658593/
https://www.ncbi.nlm.nih.gov/pubmed/36359079
http://dx.doi.org/10.3390/ani12212956
_version_ 1784829989570478080
author Chen, Jiahui
Zhang, Shuai
Chen, Genghua
Deng, Xianqi
Zhang, Danlu
Wen, Huaqiang
Yin, Yunqian
Lin, Zetong
Zhang, Xiquan
Luo, Wen
author_facet Chen, Jiahui
Zhang, Shuai
Chen, Genghua
Deng, Xianqi
Zhang, Danlu
Wen, Huaqiang
Yin, Yunqian
Lin, Zetong
Zhang, Xiquan
Luo, Wen
author_sort Chen, Jiahui
collection PubMed
description SIMPLE SUMMARY: The Shitou goose, the largest cultivated goose breed in China, has high research value in meat traits. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the mRNA and lncRNA expression profiles of Shitou goose myoblast in proliferation and differentiation were constructed by Illumina sequencing. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified between the two periods. Functional annotation showed that the DE-mRNAs and DE-lncRNAs were mainly enriched in the Wnt signaling pathway. These results provide new insights into the mechanism of muscle growth and development in large goose breeds. ABSTRACT: Chinese Shitou goose is a type of large goose with high meat yield. Understanding the genetic regulation of muscle development in Shitou goose would be beneficial to improve the meat production traits of geese. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the RNA-seq method was used to construct the mRNA and lncRNA expression profiles of Shitou goose myoblasts and myotubes. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified. The alternative mRNA splicing in proliferation and differentiation stages was also analyzed. Notably, pathways enriched in DE-mRNAs, DE-splicing transcripts, and DE-lncRNAs all point to the Wnt signaling pathway, indicating that the Wnt signaling is a key regulatory pathway of muscle development in Shitou goose. We also constructed the interactive network of DE-lncRNAs and DE-mRNAs and revealed some key genes of lncRNAs regulating the proliferation and differentiation of myoblasts. These results provide new insights for the study of the muscle development of the Shitou goose.
format Online
Article
Text
id pubmed-9658593
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96585932022-11-15 Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts Chen, Jiahui Zhang, Shuai Chen, Genghua Deng, Xianqi Zhang, Danlu Wen, Huaqiang Yin, Yunqian Lin, Zetong Zhang, Xiquan Luo, Wen Animals (Basel) Article SIMPLE SUMMARY: The Shitou goose, the largest cultivated goose breed in China, has high research value in meat traits. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the mRNA and lncRNA expression profiles of Shitou goose myoblast in proliferation and differentiation were constructed by Illumina sequencing. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified between the two periods. Functional annotation showed that the DE-mRNAs and DE-lncRNAs were mainly enriched in the Wnt signaling pathway. These results provide new insights into the mechanism of muscle growth and development in large goose breeds. ABSTRACT: Chinese Shitou goose is a type of large goose with high meat yield. Understanding the genetic regulation of muscle development in Shitou goose would be beneficial to improve the meat production traits of geese. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the RNA-seq method was used to construct the mRNA and lncRNA expression profiles of Shitou goose myoblasts and myotubes. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified. The alternative mRNA splicing in proliferation and differentiation stages was also analyzed. Notably, pathways enriched in DE-mRNAs, DE-splicing transcripts, and DE-lncRNAs all point to the Wnt signaling pathway, indicating that the Wnt signaling is a key regulatory pathway of muscle development in Shitou goose. We also constructed the interactive network of DE-lncRNAs and DE-mRNAs and revealed some key genes of lncRNAs regulating the proliferation and differentiation of myoblasts. These results provide new insights for the study of the muscle development of the Shitou goose. MDPI 2022-10-27 /pmc/articles/PMC9658593/ /pubmed/36359079 http://dx.doi.org/10.3390/ani12212956 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Jiahui
Zhang, Shuai
Chen, Genghua
Deng, Xianqi
Zhang, Danlu
Wen, Huaqiang
Yin, Yunqian
Lin, Zetong
Zhang, Xiquan
Luo, Wen
Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts
title Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts
title_full Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts
title_fullStr Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts
title_full_unstemmed Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts
title_short Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts
title_sort transcriptome sequencing reveals pathways related to proliferation and differentiation of shitou goose myoblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658593/
https://www.ncbi.nlm.nih.gov/pubmed/36359079
http://dx.doi.org/10.3390/ani12212956
work_keys_str_mv AT chenjiahui transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT zhangshuai transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT chengenghua transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT dengxianqi transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT zhangdanlu transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT wenhuaqiang transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT yinyunqian transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT linzetong transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT zhangxiquan transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts
AT luowen transcriptomesequencingrevealspathwaysrelatedtoproliferationanddifferentiationofshitougoosemyoblasts