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Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light

The effect of red (RL, 660 nm) and blue (BL, 450 nm) light on phy mutant tomato plants was studied. The rates of photosynthesis (Pn) and transpiration, the efficiency of the primary photochemical processes of photosynthesis, the contents of flavonoids and phenolic compounds, the low-molecular-weight...

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Autores principales: Pashkovskiy, Pavel, Vereshchagin, Mikhail, Kreslavski, Vladimir, Ivanov, Yury, Kumachova, Tamara, Ryabchenko, Andrey, Voronkov, Alexander, Kosobryukhov, Anatoliy, Kuznetsov, Vladimir, Allakhverdiev, Suleyman I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658692/
https://www.ncbi.nlm.nih.gov/pubmed/36359833
http://dx.doi.org/10.3390/cells11213437
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author Pashkovskiy, Pavel
Vereshchagin, Mikhail
Kreslavski, Vladimir
Ivanov, Yury
Kumachova, Tamara
Ryabchenko, Andrey
Voronkov, Alexander
Kosobryukhov, Anatoliy
Kuznetsov, Vladimir
Allakhverdiev, Suleyman I.
author_facet Pashkovskiy, Pavel
Vereshchagin, Mikhail
Kreslavski, Vladimir
Ivanov, Yury
Kumachova, Tamara
Ryabchenko, Andrey
Voronkov, Alexander
Kosobryukhov, Anatoliy
Kuznetsov, Vladimir
Allakhverdiev, Suleyman I.
author_sort Pashkovskiy, Pavel
collection PubMed
description The effect of red (RL, 660 nm) and blue (BL, 450 nm) light on phy mutant tomato plants was studied. The rates of photosynthesis (Pn) and transpiration, the efficiency of the primary photochemical processes of photosynthesis, the contents of flavonoids and phenolic compounds, the low-molecular-weight antioxidant capacity (Trolox equivalent antioxidant capacity (TEAC)) of leaf extracts, and the expression of light-dependent genes were evaluated. Under RL, BL, and white fluorescent light (WFL), the Pn values decreased in the order: WT > phyb2 > phyaphyb2 > phyaphyb1phyb2, except for the Pn in phyb2 on BL. Phyb2 also had a larger number of stomata under BL and, as a result, it reached maximum transpiration. The noticeable accumulation of flavonoids and phenolic compounds was observed only in the phyb2 and phyaphyb2 mutants upon irradiation with BL, which agrees with the increased TEAC in the leaf extracts. We suggest that the increased antioxidant activity under PHYB2 deficiency and the maintenance of high photosynthesis under BL are based on an increase in the expression of the early signaling transcription factors genes BBX, HY5. The largest decrease in the content of flavonoids and TEAC was manifested with a deficiency in PHYB1, which is probably the key to maintaining the antioxidant status in BL plants.
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spelling pubmed-96586922022-11-15 Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light Pashkovskiy, Pavel Vereshchagin, Mikhail Kreslavski, Vladimir Ivanov, Yury Kumachova, Tamara Ryabchenko, Andrey Voronkov, Alexander Kosobryukhov, Anatoliy Kuznetsov, Vladimir Allakhverdiev, Suleyman I. Cells Article The effect of red (RL, 660 nm) and blue (BL, 450 nm) light on phy mutant tomato plants was studied. The rates of photosynthesis (Pn) and transpiration, the efficiency of the primary photochemical processes of photosynthesis, the contents of flavonoids and phenolic compounds, the low-molecular-weight antioxidant capacity (Trolox equivalent antioxidant capacity (TEAC)) of leaf extracts, and the expression of light-dependent genes were evaluated. Under RL, BL, and white fluorescent light (WFL), the Pn values decreased in the order: WT > phyb2 > phyaphyb2 > phyaphyb1phyb2, except for the Pn in phyb2 on BL. Phyb2 also had a larger number of stomata under BL and, as a result, it reached maximum transpiration. The noticeable accumulation of flavonoids and phenolic compounds was observed only in the phyb2 and phyaphyb2 mutants upon irradiation with BL, which agrees with the increased TEAC in the leaf extracts. We suggest that the increased antioxidant activity under PHYB2 deficiency and the maintenance of high photosynthesis under BL are based on an increase in the expression of the early signaling transcription factors genes BBX, HY5. The largest decrease in the content of flavonoids and TEAC was manifested with a deficiency in PHYB1, which is probably the key to maintaining the antioxidant status in BL plants. MDPI 2022-10-31 /pmc/articles/PMC9658692/ /pubmed/36359833 http://dx.doi.org/10.3390/cells11213437 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pashkovskiy, Pavel
Vereshchagin, Mikhail
Kreslavski, Vladimir
Ivanov, Yury
Kumachova, Tamara
Ryabchenko, Andrey
Voronkov, Alexander
Kosobryukhov, Anatoliy
Kuznetsov, Vladimir
Allakhverdiev, Suleyman I.
Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light
title Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light
title_full Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light
title_fullStr Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light
title_full_unstemmed Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light
title_short Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light
title_sort effect of phytochrome deficiency on photosynthesis, light-related genes expression and flavonoid accumulation in solanum lycopersicum under red and blue light
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658692/
https://www.ncbi.nlm.nih.gov/pubmed/36359833
http://dx.doi.org/10.3390/cells11213437
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