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Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence

Gestational diabetes mellitus (GDM) is recognized as a “window of opportunity” for the future prediction of such complications as type 2 diabetes mellitus and pelvic floor muscle disorders, including urinary incontinence and genitourinary dysfunction. Translational studies have reported that pelvic...

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Autores principales: Alves, Fernanda Cristina Bergamo, de Oliveira, Rafael Guilen, Reyes, David Rafael Abreu, Garcia, Gabriela Azevedo, Floriano, Juliana Ferreira, Shetty, Raghavendra Hallur Lakshmana, Mareco, Edson Assunção, Dal-Pai-Silva, Maeli, Payão, Spencer Luiz Marques, de Souza, Fátima Pereira, Witkin, Steven S., Sobrevia, Luis, Barbosa, Angélica Mércia Pascon, Rudge, Marilza Vieira Cunha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658972/
https://www.ncbi.nlm.nih.gov/pubmed/36361671
http://dx.doi.org/10.3390/ijms232112864
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author Alves, Fernanda Cristina Bergamo
de Oliveira, Rafael Guilen
Reyes, David Rafael Abreu
Garcia, Gabriela Azevedo
Floriano, Juliana Ferreira
Shetty, Raghavendra Hallur Lakshmana
Mareco, Edson Assunção
Dal-Pai-Silva, Maeli
Payão, Spencer Luiz Marques
de Souza, Fátima Pereira
Witkin, Steven S.
Sobrevia, Luis
Barbosa, Angélica Mércia Pascon
Rudge, Marilza Vieira Cunha
author_facet Alves, Fernanda Cristina Bergamo
de Oliveira, Rafael Guilen
Reyes, David Rafael Abreu
Garcia, Gabriela Azevedo
Floriano, Juliana Ferreira
Shetty, Raghavendra Hallur Lakshmana
Mareco, Edson Assunção
Dal-Pai-Silva, Maeli
Payão, Spencer Luiz Marques
de Souza, Fátima Pereira
Witkin, Steven S.
Sobrevia, Luis
Barbosa, Angélica Mércia Pascon
Rudge, Marilza Vieira Cunha
author_sort Alves, Fernanda Cristina Bergamo
collection PubMed
description Gestational diabetes mellitus (GDM) is recognized as a “window of opportunity” for the future prediction of such complications as type 2 diabetes mellitus and pelvic floor muscle disorders, including urinary incontinence and genitourinary dysfunction. Translational studies have reported that pelvic floor muscle disorders are due to a GDM-induced-myopathy (GDiM) of the pelvic floor muscle and rectus abdominis muscle (RAM). We now describe the transcriptome profiling of the RAM obtained by Cesarean section from GDM and non-GDM women with and without pregnancy-specific urinary incontinence (PSUI). We identified 650 genes in total, and the differentially expressed genes were defined by comparing three control groups to the GDM with PSUI group (GDiM). Enrichment analysis showed that GDM with PSUI was associated with decreased gene expression related to muscle structure and muscle protein synthesis, the reduced ability of muscle fibers to ameliorate muscle damage, and the altered the maintenance and generation of energy through glycogenesis. Potential genetic muscle biomarkers were validated by RT-PCR, and their relationship to the pathophysiology of the disease was verified. These findings help elucidate the molecular mechanisms of GDiM and will promote the development of innovative interventions to prevent and treat complications such as post-GDM urinary incontinence.
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spelling pubmed-96589722022-11-15 Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence Alves, Fernanda Cristina Bergamo de Oliveira, Rafael Guilen Reyes, David Rafael Abreu Garcia, Gabriela Azevedo Floriano, Juliana Ferreira Shetty, Raghavendra Hallur Lakshmana Mareco, Edson Assunção Dal-Pai-Silva, Maeli Payão, Spencer Luiz Marques de Souza, Fátima Pereira Witkin, Steven S. Sobrevia, Luis Barbosa, Angélica Mércia Pascon Rudge, Marilza Vieira Cunha Int J Mol Sci Article Gestational diabetes mellitus (GDM) is recognized as a “window of opportunity” for the future prediction of such complications as type 2 diabetes mellitus and pelvic floor muscle disorders, including urinary incontinence and genitourinary dysfunction. Translational studies have reported that pelvic floor muscle disorders are due to a GDM-induced-myopathy (GDiM) of the pelvic floor muscle and rectus abdominis muscle (RAM). We now describe the transcriptome profiling of the RAM obtained by Cesarean section from GDM and non-GDM women with and without pregnancy-specific urinary incontinence (PSUI). We identified 650 genes in total, and the differentially expressed genes were defined by comparing three control groups to the GDM with PSUI group (GDiM). Enrichment analysis showed that GDM with PSUI was associated with decreased gene expression related to muscle structure and muscle protein synthesis, the reduced ability of muscle fibers to ameliorate muscle damage, and the altered the maintenance and generation of energy through glycogenesis. Potential genetic muscle biomarkers were validated by RT-PCR, and their relationship to the pathophysiology of the disease was verified. These findings help elucidate the molecular mechanisms of GDiM and will promote the development of innovative interventions to prevent and treat complications such as post-GDM urinary incontinence. MDPI 2022-10-25 /pmc/articles/PMC9658972/ /pubmed/36361671 http://dx.doi.org/10.3390/ijms232112864 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alves, Fernanda Cristina Bergamo
de Oliveira, Rafael Guilen
Reyes, David Rafael Abreu
Garcia, Gabriela Azevedo
Floriano, Juliana Ferreira
Shetty, Raghavendra Hallur Lakshmana
Mareco, Edson Assunção
Dal-Pai-Silva, Maeli
Payão, Spencer Luiz Marques
de Souza, Fátima Pereira
Witkin, Steven S.
Sobrevia, Luis
Barbosa, Angélica Mércia Pascon
Rudge, Marilza Vieira Cunha
Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
title Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
title_full Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
title_fullStr Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
title_full_unstemmed Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
title_short Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
title_sort transcriptomic profiling of rectus abdominis muscle in women with gestational diabetes-induced myopathy: characterization of pathophysiology and potential muscle biomarkers of pregnancy-specific urinary incontinence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658972/
https://www.ncbi.nlm.nih.gov/pubmed/36361671
http://dx.doi.org/10.3390/ijms232112864
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