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Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)

Fruit size is an important fruit quality trait that influences the production and commodity values of loquats (Eriobotrya japonica Lindl.). The Small Auxin Upregulated RNA (SAUR) gene family has proven to play a vital role in the fruit development of many plant species. However, it has not been comp...

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Autores principales: Peng, Ze, Li, Wenxiang, Gan, Xiaoqing, Zhao, Chongbin, Paudel, Dev, Su, Wenbing, Lv, Juan, Lin, Shunquan, Liu, Zongli, Yang, Xianghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659022/
https://www.ncbi.nlm.nih.gov/pubmed/36362065
http://dx.doi.org/10.3390/ijms232113271
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author Peng, Ze
Li, Wenxiang
Gan, Xiaoqing
Zhao, Chongbin
Paudel, Dev
Su, Wenbing
Lv, Juan
Lin, Shunquan
Liu, Zongli
Yang, Xianghui
author_facet Peng, Ze
Li, Wenxiang
Gan, Xiaoqing
Zhao, Chongbin
Paudel, Dev
Su, Wenbing
Lv, Juan
Lin, Shunquan
Liu, Zongli
Yang, Xianghui
author_sort Peng, Ze
collection PubMed
description Fruit size is an important fruit quality trait that influences the production and commodity values of loquats (Eriobotrya japonica Lindl.). The Small Auxin Upregulated RNA (SAUR) gene family has proven to play a vital role in the fruit development of many plant species. However, it has not been comprehensively studied in a genome-wide manner in loquats, and its role in regulating fruit size remains unknown. In this study, we identified 95 EjSAUR genes in the loquat genome. Tandem duplication and segmental duplication contributed to the expansion of this gene family in loquats. Phylogenetic analysis grouped the SAURs from Arabidopsis, rice, and loquat into nine clusters. By analyzing the transcriptome profiles in different tissues and at different fruit developmental stages and comparing two sister lines with contrasting fruit sizes, as well as by functional predictions, a candidate gene (EjSAUR22) highly expressed in expanding fruits was selected for further functional investigation. A combination of Indoleacetic acid (IAA) treatment and virus-induced gene silencing revealed that EjSAUR22 was not only responsive to auxin, but also played a role in regulating cell size and fruit expansion. The findings from our study provide a solid foundation for understanding the molecular mechanisms controlling fruit size in loquats, and also provide potential targets for manipulation of fruit size to accelerate loquat breeding.
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spelling pubmed-96590222022-11-15 Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.) Peng, Ze Li, Wenxiang Gan, Xiaoqing Zhao, Chongbin Paudel, Dev Su, Wenbing Lv, Juan Lin, Shunquan Liu, Zongli Yang, Xianghui Int J Mol Sci Article Fruit size is an important fruit quality trait that influences the production and commodity values of loquats (Eriobotrya japonica Lindl.). The Small Auxin Upregulated RNA (SAUR) gene family has proven to play a vital role in the fruit development of many plant species. However, it has not been comprehensively studied in a genome-wide manner in loquats, and its role in regulating fruit size remains unknown. In this study, we identified 95 EjSAUR genes in the loquat genome. Tandem duplication and segmental duplication contributed to the expansion of this gene family in loquats. Phylogenetic analysis grouped the SAURs from Arabidopsis, rice, and loquat into nine clusters. By analyzing the transcriptome profiles in different tissues and at different fruit developmental stages and comparing two sister lines with contrasting fruit sizes, as well as by functional predictions, a candidate gene (EjSAUR22) highly expressed in expanding fruits was selected for further functional investigation. A combination of Indoleacetic acid (IAA) treatment and virus-induced gene silencing revealed that EjSAUR22 was not only responsive to auxin, but also played a role in regulating cell size and fruit expansion. The findings from our study provide a solid foundation for understanding the molecular mechanisms controlling fruit size in loquats, and also provide potential targets for manipulation of fruit size to accelerate loquat breeding. MDPI 2022-10-31 /pmc/articles/PMC9659022/ /pubmed/36362065 http://dx.doi.org/10.3390/ijms232113271 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peng, Ze
Li, Wenxiang
Gan, Xiaoqing
Zhao, Chongbin
Paudel, Dev
Su, Wenbing
Lv, Juan
Lin, Shunquan
Liu, Zongli
Yang, Xianghui
Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)
title Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)
title_full Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)
title_fullStr Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)
title_full_unstemmed Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)
title_short Genome-Wide Analysis of SAUR Gene Family Identifies a Candidate Associated with Fruit Size in Loquat (Eriobotrya japonica Lindl.)
title_sort genome-wide analysis of saur gene family identifies a candidate associated with fruit size in loquat (eriobotrya japonica lindl.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659022/
https://www.ncbi.nlm.nih.gov/pubmed/36362065
http://dx.doi.org/10.3390/ijms232113271
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