Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids

In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based...

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Detalles Bibliográficos
Autores principales: Kukushkin, Vladimir, Ambartsumyan, Oganes, Astrakhantseva, Anna, Gushchin, Vladimir, Nikonova, Alexandra, Dorofeeva, Anastasia, Zverev, Vitaly, Gambaryan, Alexandra, Tikhonova, Daria, Sovetnikov, Timofei, Akhmetova, Assel, Yaminsky, Igor, Zavyalova, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659100/
https://www.ncbi.nlm.nih.gov/pubmed/36364630
http://dx.doi.org/10.3390/nano12213854
Descripción
Sumario:In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based on a reproducible lithographic SERS substrate, featuring the combination of high speed, specificity, and ultrasensitive quantitative detection of SARS-CoV-2 virions. The sensor makes it possible to identify SARS-CoV-2 in very low concentrations (the limit of detection was 100 copies/mL), demonstrating a sensitivity level comparable to the existing diagnostic golden standard—the reverse transcription polymerase chain reaction.

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