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Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex

Itch and pain are distinct sensations that share anatomically similar pathways: from the periphery to the brain. Over the last decades, several itch-specific neural pathways and molecular markers have been identified at the peripheral and spinal cord levels. Although the perception of sensation is u...

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Autores principales: Woo, Seunghui, Kim, Yoo Rim, Bak, Myeong Seong, Chung, Geehoon, Kim, Sang Jeong, Kim, Sun Kwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Brain and Neural Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659493/
https://www.ncbi.nlm.nih.gov/pubmed/36351842
http://dx.doi.org/10.5607/en22029
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author Woo, Seunghui
Kim, Yoo Rim
Bak, Myeong Seong
Chung, Geehoon
Kim, Sang Jeong
Kim, Sun Kwang
author_facet Woo, Seunghui
Kim, Yoo Rim
Bak, Myeong Seong
Chung, Geehoon
Kim, Sang Jeong
Kim, Sun Kwang
author_sort Woo, Seunghui
collection PubMed
description Itch and pain are distinct sensations that share anatomically similar pathways: from the periphery to the brain. Over the last decades, several itch-specific neural pathways and molecular markers have been identified at the peripheral and spinal cord levels. Although the perception of sensation is ultimately generated at the brain level, how the brain separately processes the signals is unclear. The primary somatosensory cortex (S1) plays a crucial role in the perception of somatosensory information, including touch, itch, and pain. In this study, we investigated how S1 neurons represent itch and pain differently. First, we established a spontaneous itch and pain mouse model. Spontaneous itch or pain was induced by intradermal treatment with 5-HT or capsaicin on the lateral neck and confirmed by a selective increase in scratching or wiping-like behavior, respectively. Next, in vivo two-photon calcium imaging was performed in awake mice after four different treatments, including 5-HT, capsaicin, and each vehicle. By comparing the calcium activity acquired during different sessions, we distinguished the cells responsive to itch or pain sensations. Of the total responsive cells, 11% were both responsive, and their activity in the pain session was slightly higher than that in the itch session. Itch- and pain-preferred cells accounted for 28.4% and 60.6%, respectively, and the preferred cells showed the lowest activity in their counter sessions. Therefore, our results suggest that S1 uses a multiplexed coding strategy to encode itch and pain, and S1 neurons represent the interaction between itch and pain.
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spelling pubmed-96594932022-11-22 Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex Woo, Seunghui Kim, Yoo Rim Bak, Myeong Seong Chung, Geehoon Kim, Sang Jeong Kim, Sun Kwang Exp Neurobiol Short Communication Itch and pain are distinct sensations that share anatomically similar pathways: from the periphery to the brain. Over the last decades, several itch-specific neural pathways and molecular markers have been identified at the peripheral and spinal cord levels. Although the perception of sensation is ultimately generated at the brain level, how the brain separately processes the signals is unclear. The primary somatosensory cortex (S1) plays a crucial role in the perception of somatosensory information, including touch, itch, and pain. In this study, we investigated how S1 neurons represent itch and pain differently. First, we established a spontaneous itch and pain mouse model. Spontaneous itch or pain was induced by intradermal treatment with 5-HT or capsaicin on the lateral neck and confirmed by a selective increase in scratching or wiping-like behavior, respectively. Next, in vivo two-photon calcium imaging was performed in awake mice after four different treatments, including 5-HT, capsaicin, and each vehicle. By comparing the calcium activity acquired during different sessions, we distinguished the cells responsive to itch or pain sensations. Of the total responsive cells, 11% were both responsive, and their activity in the pain session was slightly higher than that in the itch session. Itch- and pain-preferred cells accounted for 28.4% and 60.6%, respectively, and the preferred cells showed the lowest activity in their counter sessions. Therefore, our results suggest that S1 uses a multiplexed coding strategy to encode itch and pain, and S1 neurons represent the interaction between itch and pain. The Korean Society for Brain and Neural Sciences 2022-10-31 2022-10-31 /pmc/articles/PMC9659493/ /pubmed/36351842 http://dx.doi.org/10.5607/en22029 Text en Copyright © Experimental Neurobiology 2021 https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Woo, Seunghui
Kim, Yoo Rim
Bak, Myeong Seong
Chung, Geehoon
Kim, Sang Jeong
Kim, Sun Kwang
Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex
title Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex
title_full Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex
title_fullStr Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex
title_full_unstemmed Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex
title_short Multiplexed Representation of Itch and Pain and Their Interaction in the Primary Somatosensory Cortex
title_sort multiplexed representation of itch and pain and their interaction in the primary somatosensory cortex
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659493/
https://www.ncbi.nlm.nih.gov/pubmed/36351842
http://dx.doi.org/10.5607/en22029
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