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Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection
Many species of Inosperma cause neurotoxic poisoning in humans after consumption around the world. However, the toxic species of Inosperma and its toxin content remain unclear. In the present study, we proposed five new Inosperma species from China, namely, I. longisporum, I. nivalellum, I. sphaerob...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659589/ https://www.ncbi.nlm.nih.gov/pubmed/36386664 http://dx.doi.org/10.3389/fmicb.2022.1021583 |
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author | Li, Sai-Nan Xu, Fei Long, Pan Liu, Feng Zhang, Ping Fan, Yu-Guang Chen, Zuo-Hong |
author_facet | Li, Sai-Nan Xu, Fei Long, Pan Liu, Feng Zhang, Ping Fan, Yu-Guang Chen, Zuo-Hong |
author_sort | Li, Sai-Nan |
collection | PubMed |
description | Many species of Inosperma cause neurotoxic poisoning in humans after consumption around the world. However, the toxic species of Inosperma and its toxin content remain unclear. In the present study, we proposed five new Inosperma species from China, namely, I. longisporum, I. nivalellum, I. sphaerobulbosum, I. squamulosobrunneum, and I. squamulosohinnuleum. Morphological and molecular phylogenetic analyses based on three genes (ITS, nrLSU, rpb2) revealed that these taxa are independent species. A key to 17 species of Inosperma in China is provided. In addition, targeted screening for the most notorious mushroom neurotoxins, muscarine, psilocybin, ibotenic acid, and muscimol, in these five new species was performed by using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Our results show that the neurotoxin contents in these five species varied: I. sphaerobulbosum contains none of the tested neurotoxins; I. nivalellum is muscarine positive; I. longisporum and I. squamulosohinnuleum contain both ibotenic acid and muscimol, and I. squamulosobrunneum only contains muscimol; psilocybin was not detected in these five new species. |
format | Online Article Text |
id | pubmed-9659589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96595892022-11-15 Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection Li, Sai-Nan Xu, Fei Long, Pan Liu, Feng Zhang, Ping Fan, Yu-Guang Chen, Zuo-Hong Front Microbiol Microbiology Many species of Inosperma cause neurotoxic poisoning in humans after consumption around the world. However, the toxic species of Inosperma and its toxin content remain unclear. In the present study, we proposed five new Inosperma species from China, namely, I. longisporum, I. nivalellum, I. sphaerobulbosum, I. squamulosobrunneum, and I. squamulosohinnuleum. Morphological and molecular phylogenetic analyses based on three genes (ITS, nrLSU, rpb2) revealed that these taxa are independent species. A key to 17 species of Inosperma in China is provided. In addition, targeted screening for the most notorious mushroom neurotoxins, muscarine, psilocybin, ibotenic acid, and muscimol, in these five new species was performed by using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Our results show that the neurotoxin contents in these five species varied: I. sphaerobulbosum contains none of the tested neurotoxins; I. nivalellum is muscarine positive; I. longisporum and I. squamulosohinnuleum contain both ibotenic acid and muscimol, and I. squamulosobrunneum only contains muscimol; psilocybin was not detected in these five new species. Frontiers Media S.A. 2022-10-31 /pmc/articles/PMC9659589/ /pubmed/36386664 http://dx.doi.org/10.3389/fmicb.2022.1021583 Text en Copyright © 2022 Li, Xu, Long, Liu, Zhang, Fan and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Sai-Nan Xu, Fei Long, Pan Liu, Feng Zhang, Ping Fan, Yu-Guang Chen, Zuo-Hong Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection |
title | Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection |
title_full | Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection |
title_fullStr | Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection |
title_full_unstemmed | Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection |
title_short | Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection |
title_sort | five new species of inosperma from china: morphological characteristics, phylogenetic analyses, and toxin detection |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659589/ https://www.ncbi.nlm.nih.gov/pubmed/36386664 http://dx.doi.org/10.3389/fmicb.2022.1021583 |
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