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Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model

The development of 3D scaffold-based models would represent a great step forward in cancer research, offering the possibility of predicting the potential in vivo response to targeted anticancer or anti-angiogenic therapies. As regards, 3D in vitro models require proper materials, which faithfully re...

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Autores principales: Grilli, Francesca, Pitton, Matteo, Altomare, Lina, Farè, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659726/
https://www.ncbi.nlm.nih.gov/pubmed/36394006
http://dx.doi.org/10.3389/fbioe.2022.984805
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author Grilli, Francesca
Pitton, Matteo
Altomare, Lina
Farè, Silvia
author_facet Grilli, Francesca
Pitton, Matteo
Altomare, Lina
Farè, Silvia
author_sort Grilli, Francesca
collection PubMed
description The development of 3D scaffold-based models would represent a great step forward in cancer research, offering the possibility of predicting the potential in vivo response to targeted anticancer or anti-angiogenic therapies. As regards, 3D in vitro models require proper materials, which faithfully recapitulated extracellular matrix (ECM) properties, adequate cell lines, and an efficient vascular network. The aim of this work is to investigate the possible realization of an in vitro 3D scaffold-based model of adipose tissue, by incorporating decellularized 3D plant structures within the scaffold. In particular, in order to obtain an adipose matrix capable of mimicking the composition of the adipose tissue, methacrylated gelatin (GelMA), UV photo-crosslinkable, was selected. Decellularized fennel, wild fennel and, dill leaves have been incorporated into the GelMA hydrogel before crosslinking, to mimic a 3D channel network. All leaves showed a loss of pigmentation after the decellularization with channel dimensions ranging from 100 to 500 µm up to 3 μm, comparable with those of human microcirculation (5–10 µm). The photo-crosslinking process was not affected by the embedded plant structures in GelMA hydrogels. In fact, the weight variation test, performed on hydrogels with or without decellularized leaves showed a weight loss in the first 96 h, followed by a stability plateau up to 5 weeks. No cytotoxic effects were detected comparing the three prepared GelMA/D-leaf structures; moreover, the ability of the samples to stimulate differentiation of 3T3-L1 preadipocytes in mature adipocytes was investigated, and cells were able to grow and proliferate in the structure, colonizing the entire microenvironment and starting to differentiate. The developed GelMA hydrogels mimicked adipose tissue together with the incorporated plant structures seem to be an adequate solution to ensure an efficient vascular system for a 3D in vitro model. The obtained results showed the potentiality of the innovative proposed approach to mimic the tumoral microenvironment in 3D scaffold-based models.
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spelling pubmed-96597262022-11-15 Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model Grilli, Francesca Pitton, Matteo Altomare, Lina Farè, Silvia Front Bioeng Biotechnol Bioengineering and Biotechnology The development of 3D scaffold-based models would represent a great step forward in cancer research, offering the possibility of predicting the potential in vivo response to targeted anticancer or anti-angiogenic therapies. As regards, 3D in vitro models require proper materials, which faithfully recapitulated extracellular matrix (ECM) properties, adequate cell lines, and an efficient vascular network. The aim of this work is to investigate the possible realization of an in vitro 3D scaffold-based model of adipose tissue, by incorporating decellularized 3D plant structures within the scaffold. In particular, in order to obtain an adipose matrix capable of mimicking the composition of the adipose tissue, methacrylated gelatin (GelMA), UV photo-crosslinkable, was selected. Decellularized fennel, wild fennel and, dill leaves have been incorporated into the GelMA hydrogel before crosslinking, to mimic a 3D channel network. All leaves showed a loss of pigmentation after the decellularization with channel dimensions ranging from 100 to 500 µm up to 3 μm, comparable with those of human microcirculation (5–10 µm). The photo-crosslinking process was not affected by the embedded plant structures in GelMA hydrogels. In fact, the weight variation test, performed on hydrogels with or without decellularized leaves showed a weight loss in the first 96 h, followed by a stability plateau up to 5 weeks. No cytotoxic effects were detected comparing the three prepared GelMA/D-leaf structures; moreover, the ability of the samples to stimulate differentiation of 3T3-L1 preadipocytes in mature adipocytes was investigated, and cells were able to grow and proliferate in the structure, colonizing the entire microenvironment and starting to differentiate. The developed GelMA hydrogels mimicked adipose tissue together with the incorporated plant structures seem to be an adequate solution to ensure an efficient vascular system for a 3D in vitro model. The obtained results showed the potentiality of the innovative proposed approach to mimic the tumoral microenvironment in 3D scaffold-based models. Frontiers Media S.A. 2022-10-31 /pmc/articles/PMC9659726/ /pubmed/36394006 http://dx.doi.org/10.3389/fbioe.2022.984805 Text en Copyright © 2022 Grilli, Pitton, Altomare and Farè. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Grilli, Francesca
Pitton, Matteo
Altomare, Lina
Farè, Silvia
Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
title Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
title_full Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
title_fullStr Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
title_full_unstemmed Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
title_short Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
title_sort decellularized fennel and dill leaves as possible 3d channel network in gelma for the development of an in vitro adipose tissue model
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659726/
https://www.ncbi.nlm.nih.gov/pubmed/36394006
http://dx.doi.org/10.3389/fbioe.2022.984805
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