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Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model
The development of 3D scaffold-based models would represent a great step forward in cancer research, offering the possibility of predicting the potential in vivo response to targeted anticancer or anti-angiogenic therapies. As regards, 3D in vitro models require proper materials, which faithfully re...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659726/ https://www.ncbi.nlm.nih.gov/pubmed/36394006 http://dx.doi.org/10.3389/fbioe.2022.984805 |
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author | Grilli, Francesca Pitton, Matteo Altomare, Lina Farè, Silvia |
author_facet | Grilli, Francesca Pitton, Matteo Altomare, Lina Farè, Silvia |
author_sort | Grilli, Francesca |
collection | PubMed |
description | The development of 3D scaffold-based models would represent a great step forward in cancer research, offering the possibility of predicting the potential in vivo response to targeted anticancer or anti-angiogenic therapies. As regards, 3D in vitro models require proper materials, which faithfully recapitulated extracellular matrix (ECM) properties, adequate cell lines, and an efficient vascular network. The aim of this work is to investigate the possible realization of an in vitro 3D scaffold-based model of adipose tissue, by incorporating decellularized 3D plant structures within the scaffold. In particular, in order to obtain an adipose matrix capable of mimicking the composition of the adipose tissue, methacrylated gelatin (GelMA), UV photo-crosslinkable, was selected. Decellularized fennel, wild fennel and, dill leaves have been incorporated into the GelMA hydrogel before crosslinking, to mimic a 3D channel network. All leaves showed a loss of pigmentation after the decellularization with channel dimensions ranging from 100 to 500 µm up to 3 μm, comparable with those of human microcirculation (5–10 µm). The photo-crosslinking process was not affected by the embedded plant structures in GelMA hydrogels. In fact, the weight variation test, performed on hydrogels with or without decellularized leaves showed a weight loss in the first 96 h, followed by a stability plateau up to 5 weeks. No cytotoxic effects were detected comparing the three prepared GelMA/D-leaf structures; moreover, the ability of the samples to stimulate differentiation of 3T3-L1 preadipocytes in mature adipocytes was investigated, and cells were able to grow and proliferate in the structure, colonizing the entire microenvironment and starting to differentiate. The developed GelMA hydrogels mimicked adipose tissue together with the incorporated plant structures seem to be an adequate solution to ensure an efficient vascular system for a 3D in vitro model. The obtained results showed the potentiality of the innovative proposed approach to mimic the tumoral microenvironment in 3D scaffold-based models. |
format | Online Article Text |
id | pubmed-9659726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96597262022-11-15 Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model Grilli, Francesca Pitton, Matteo Altomare, Lina Farè, Silvia Front Bioeng Biotechnol Bioengineering and Biotechnology The development of 3D scaffold-based models would represent a great step forward in cancer research, offering the possibility of predicting the potential in vivo response to targeted anticancer or anti-angiogenic therapies. As regards, 3D in vitro models require proper materials, which faithfully recapitulated extracellular matrix (ECM) properties, adequate cell lines, and an efficient vascular network. The aim of this work is to investigate the possible realization of an in vitro 3D scaffold-based model of adipose tissue, by incorporating decellularized 3D plant structures within the scaffold. In particular, in order to obtain an adipose matrix capable of mimicking the composition of the adipose tissue, methacrylated gelatin (GelMA), UV photo-crosslinkable, was selected. Decellularized fennel, wild fennel and, dill leaves have been incorporated into the GelMA hydrogel before crosslinking, to mimic a 3D channel network. All leaves showed a loss of pigmentation after the decellularization with channel dimensions ranging from 100 to 500 µm up to 3 μm, comparable with those of human microcirculation (5–10 µm). The photo-crosslinking process was not affected by the embedded plant structures in GelMA hydrogels. In fact, the weight variation test, performed on hydrogels with or without decellularized leaves showed a weight loss in the first 96 h, followed by a stability plateau up to 5 weeks. No cytotoxic effects were detected comparing the three prepared GelMA/D-leaf structures; moreover, the ability of the samples to stimulate differentiation of 3T3-L1 preadipocytes in mature adipocytes was investigated, and cells were able to grow and proliferate in the structure, colonizing the entire microenvironment and starting to differentiate. The developed GelMA hydrogels mimicked adipose tissue together with the incorporated plant structures seem to be an adequate solution to ensure an efficient vascular system for a 3D in vitro model. The obtained results showed the potentiality of the innovative proposed approach to mimic the tumoral microenvironment in 3D scaffold-based models. Frontiers Media S.A. 2022-10-31 /pmc/articles/PMC9659726/ /pubmed/36394006 http://dx.doi.org/10.3389/fbioe.2022.984805 Text en Copyright © 2022 Grilli, Pitton, Altomare and Farè. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Grilli, Francesca Pitton, Matteo Altomare, Lina Farè, Silvia Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model |
title | Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model |
title_full | Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model |
title_fullStr | Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model |
title_full_unstemmed | Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model |
title_short | Decellularized fennel and dill leaves as possible 3D channel network in GelMA for the development of an in vitro adipose tissue model |
title_sort | decellularized fennel and dill leaves as possible 3d channel network in gelma for the development of an in vitro adipose tissue model |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659726/ https://www.ncbi.nlm.nih.gov/pubmed/36394006 http://dx.doi.org/10.3389/fbioe.2022.984805 |
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