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Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria

Sensing surface topography, an upsurge of signaling biomolecules, and upholding cellular homeostasis are the rate-limiting spatio-temporal events in microbial attachment and biofilm formation. Initially, a set of highly specialized proteins, viz. conditioning protein, directs the irreversible attach...

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Autores principales: Raya, Dheeraj, Shreya, Aritree, Kumar, Anil, Giri, Shiv Kumar, Salem, David R., Gnimpieba, Etienne Z., Gadhamshetty, Venkataramana, Dhiman, Saurabh Sudha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659907/
https://www.ncbi.nlm.nih.gov/pubmed/36386676
http://dx.doi.org/10.3389/fmicb.2022.1008536
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author Raya, Dheeraj
Shreya, Aritree
Kumar, Anil
Giri, Shiv Kumar
Salem, David R.
Gnimpieba, Etienne Z.
Gadhamshetty, Venkataramana
Dhiman, Saurabh Sudha
author_facet Raya, Dheeraj
Shreya, Aritree
Kumar, Anil
Giri, Shiv Kumar
Salem, David R.
Gnimpieba, Etienne Z.
Gadhamshetty, Venkataramana
Dhiman, Saurabh Sudha
author_sort Raya, Dheeraj
collection PubMed
description Sensing surface topography, an upsurge of signaling biomolecules, and upholding cellular homeostasis are the rate-limiting spatio-temporal events in microbial attachment and biofilm formation. Initially, a set of highly specialized proteins, viz. conditioning protein, directs the irreversible attachment of the microbes. Later signaling molecules, viz. autoinducer, take over the cellular communication phenomenon, resulting in a mature microbial biofilm. The mandatory release of conditioning proteins and autoinducers corroborated the existence of two independent mechanisms operating sequentially for biofilm development. However, both these mechanisms are significantly affected by the availability of the cofactor, e.g., Copper (Cu). Generally, the Cu concentration beyond threshold levels is detrimental to the anaerobes except for a few species of sulfate-reducing bacteria (SRB). Remarkably SRB has developed intricate ways to resist and thrive in the presence of Cu by activating numerous genes responsible for modifying the presence of more toxic Cu(I) to Cu(II) within the periplasm, followed by their export through the outer membrane. Therefore, the determinants of Cu toxicity, sequestration, and transportation are reconnoitered for their contribution towards microbial adaptations and biofilm formation. The mechanistic details revealing Cu as a quorum quencher (QQ) are provided in addition to the three pathways involved in the dissolution of cellular communications. This review articulates the Machine Learning based data curing and data processing for designing novel anti-biofilm peptides and for an in-depth understanding of QQ mechanisms. A pioneering data set has been mined and presented on the functional properties of the QQ homolog in Oleidesulfovibrio alaskensis G20 and residues regulating the multicopper oxidase properties in SRB.
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spelling pubmed-96599072022-11-15 Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria Raya, Dheeraj Shreya, Aritree Kumar, Anil Giri, Shiv Kumar Salem, David R. Gnimpieba, Etienne Z. Gadhamshetty, Venkataramana Dhiman, Saurabh Sudha Front Microbiol Microbiology Sensing surface topography, an upsurge of signaling biomolecules, and upholding cellular homeostasis are the rate-limiting spatio-temporal events in microbial attachment and biofilm formation. Initially, a set of highly specialized proteins, viz. conditioning protein, directs the irreversible attachment of the microbes. Later signaling molecules, viz. autoinducer, take over the cellular communication phenomenon, resulting in a mature microbial biofilm. The mandatory release of conditioning proteins and autoinducers corroborated the existence of two independent mechanisms operating sequentially for biofilm development. However, both these mechanisms are significantly affected by the availability of the cofactor, e.g., Copper (Cu). Generally, the Cu concentration beyond threshold levels is detrimental to the anaerobes except for a few species of sulfate-reducing bacteria (SRB). Remarkably SRB has developed intricate ways to resist and thrive in the presence of Cu by activating numerous genes responsible for modifying the presence of more toxic Cu(I) to Cu(II) within the periplasm, followed by their export through the outer membrane. Therefore, the determinants of Cu toxicity, sequestration, and transportation are reconnoitered for their contribution towards microbial adaptations and biofilm formation. The mechanistic details revealing Cu as a quorum quencher (QQ) are provided in addition to the three pathways involved in the dissolution of cellular communications. This review articulates the Machine Learning based data curing and data processing for designing novel anti-biofilm peptides and for an in-depth understanding of QQ mechanisms. A pioneering data set has been mined and presented on the functional properties of the QQ homolog in Oleidesulfovibrio alaskensis G20 and residues regulating the multicopper oxidase properties in SRB. Frontiers Media S.A. 2022-10-31 /pmc/articles/PMC9659907/ /pubmed/36386676 http://dx.doi.org/10.3389/fmicb.2022.1008536 Text en Copyright © 2022 Raya, Shreya, Kumar, Giri, Salem, Gnimpieba, Gadhamshetty and Dhiman. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Raya, Dheeraj
Shreya, Aritree
Kumar, Anil
Giri, Shiv Kumar
Salem, David R.
Gnimpieba, Etienne Z.
Gadhamshetty, Venkataramana
Dhiman, Saurabh Sudha
Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
title Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
title_full Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
title_fullStr Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
title_full_unstemmed Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
title_short Molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
title_sort molecular regulation of conditioning film formation and quorum quenching in sulfate reducing bacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659907/
https://www.ncbi.nlm.nih.gov/pubmed/36386676
http://dx.doi.org/10.3389/fmicb.2022.1008536
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