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LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo

BACKGROUND: This study aimed to determine the role of LINC00178 in colorectal cancer (CRC) cell invasion and migration by examining its expression in CRC cells and tissues. METHODS: Cancer tissues and corresponding adjacent tissue specimens were collected from 45 patients who experienced radical CRC...

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Autores principales: Ma, Kun, Shao, Zefeng, Xu, Xiangxian, Guo, Shanfeng, Wang, Youjin, Wang, Zhongqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9660056/
https://www.ncbi.nlm.nih.gov/pubmed/36388674
http://dx.doi.org/10.21037/jgo-22-940
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author Ma, Kun
Shao, Zefeng
Xu, Xiangxian
Guo, Shanfeng
Wang, Youjin
Wang, Zhongqiu
author_facet Ma, Kun
Shao, Zefeng
Xu, Xiangxian
Guo, Shanfeng
Wang, Youjin
Wang, Zhongqiu
author_sort Ma, Kun
collection PubMed
description BACKGROUND: This study aimed to determine the role of LINC00178 in colorectal cancer (CRC) cell invasion and migration by examining its expression in CRC cells and tissues. METHODS: Cancer tissues and corresponding adjacent tissue specimens were collected from 45 patients who experienced radical CRC resection in the hospital from March to September 2021. The expression of LINC00178 was measured in both CRC cells and tissues and normal human colorectal mucosal cells using quantitative fluorescence polymerase chain reaction (QF-PCR). Cell Counting Kit-8 (CCK-8), clonogenic, and transwell assays were used to assess the impact of LINC00178 overexpression or knockdown on the CRC cells invasion and proliferation. In addition, the expression levels of vimentin, E-cadherin, and N-cadherin in CRC cells were determined after either LINC00178 knockdown or overexpression was performed using western blotting. RESULTS: The experiments revealed that LINC00178 was over expressed in CRC cells and tissues. Over-expression of LINC00178 could significantly promote the propagation, clone formation, invasion, and transportation of CRC, whereas knockdown of LINC00178 had the opposite function. When LINC00178 was expressed at high levels, it suppressed the vimentin and N-cadherin expression and prevented the upregulation of E-cadherin. In vivo (nude mouse) studies showed that the over expression of LINC00178 could significantly promote the propagation in CRC cells. CONCLUSIONS: LINC00178 is overexpressed in CRC cells and tissues. In vivo and in vitro experiments showed that LINC00178 can significantly promote the propagation of CRC cells, so it may develop a potential biological site for targeted therapy of CRC patients.
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spelling pubmed-96600562022-11-15 LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo Ma, Kun Shao, Zefeng Xu, Xiangxian Guo, Shanfeng Wang, Youjin Wang, Zhongqiu J Gastrointest Oncol Original Article BACKGROUND: This study aimed to determine the role of LINC00178 in colorectal cancer (CRC) cell invasion and migration by examining its expression in CRC cells and tissues. METHODS: Cancer tissues and corresponding adjacent tissue specimens were collected from 45 patients who experienced radical CRC resection in the hospital from March to September 2021. The expression of LINC00178 was measured in both CRC cells and tissues and normal human colorectal mucosal cells using quantitative fluorescence polymerase chain reaction (QF-PCR). Cell Counting Kit-8 (CCK-8), clonogenic, and transwell assays were used to assess the impact of LINC00178 overexpression or knockdown on the CRC cells invasion and proliferation. In addition, the expression levels of vimentin, E-cadherin, and N-cadherin in CRC cells were determined after either LINC00178 knockdown or overexpression was performed using western blotting. RESULTS: The experiments revealed that LINC00178 was over expressed in CRC cells and tissues. Over-expression of LINC00178 could significantly promote the propagation, clone formation, invasion, and transportation of CRC, whereas knockdown of LINC00178 had the opposite function. When LINC00178 was expressed at high levels, it suppressed the vimentin and N-cadherin expression and prevented the upregulation of E-cadherin. In vivo (nude mouse) studies showed that the over expression of LINC00178 could significantly promote the propagation in CRC cells. CONCLUSIONS: LINC00178 is overexpressed in CRC cells and tissues. In vivo and in vitro experiments showed that LINC00178 can significantly promote the propagation of CRC cells, so it may develop a potential biological site for targeted therapy of CRC patients. AME Publishing Company 2022-10 /pmc/articles/PMC9660056/ /pubmed/36388674 http://dx.doi.org/10.21037/jgo-22-940 Text en 2022 Journal of Gastrointestinal Oncology. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Ma, Kun
Shao, Zefeng
Xu, Xiangxian
Guo, Shanfeng
Wang, Youjin
Wang, Zhongqiu
LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
title LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
title_full LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
title_fullStr LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
title_full_unstemmed LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
title_short LINC00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
title_sort linc00178 promotes cell growth, migration, and invasion in colorectal cancer in vitro and in vivo
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9660056/
https://www.ncbi.nlm.nih.gov/pubmed/36388674
http://dx.doi.org/10.21037/jgo-22-940
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