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Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever

Ultrasensitive molecular detection and quantization are crucial for many applications including clinical diagnostics, functional proteomics, and drug discovery; however, conventional biochemical sensors cannot satisfy the stringent requirements, and this has resulted in a long-standing dilemma regar...

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Autores principales: Rao, Depeng, Yan, Tianhao, Qiao, Zihan, Wang, Yu, Peng, Yongpei, Tu, Han, Wu, Shangquan, Zhang, Qingchuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tsinghua University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661467/
https://www.ncbi.nlm.nih.gov/pubmed/36405983
http://dx.doi.org/10.1007/s12274-022-5049-0
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author Rao, Depeng
Yan, Tianhao
Qiao, Zihan
Wang, Yu
Peng, Yongpei
Tu, Han
Wu, Shangquan
Zhang, Qingchuan
author_facet Rao, Depeng
Yan, Tianhao
Qiao, Zihan
Wang, Yu
Peng, Yongpei
Tu, Han
Wu, Shangquan
Zhang, Qingchuan
author_sort Rao, Depeng
collection PubMed
description Ultrasensitive molecular detection and quantization are crucial for many applications including clinical diagnostics, functional proteomics, and drug discovery; however, conventional biochemical sensors cannot satisfy the stringent requirements, and this has resulted in a long-standing dilemma regarding sensitivity improvement. To this end, we have developed an ultrasensitive relay-type nanomechanical sensor based on a magneto lever. By establishing the link between very weak molecular interaction and five orders of magnitude larger magnetic force, analytes at ultratrace level can produce a clearly observable mechanical response. Initially, proof-of-concept studies showed an improved detection limit up to five orders of magnitude when employing the magneto lever, as compared with direct detection using probe alone. In this study, we subsequently demonstrated that the relay-type sensing mode was universal in application ranging from micromolecule to macromolecule detection, which can be easily extended to detect enzymes, DNA, proteins, cells, viruses, bacteria, chemicals, etc. Importantly, we found that, sensitivity was no longer subject to probe affinity when the magneto lever was sufficiently high, theoretically, even reaching single-molecule resolution. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material (experimental section) is available in the online version of this article at 10.1007/s12274-022-5049-0.
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spelling pubmed-96614672022-11-14 Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever Rao, Depeng Yan, Tianhao Qiao, Zihan Wang, Yu Peng, Yongpei Tu, Han Wu, Shangquan Zhang, Qingchuan Nano Res Research Article Ultrasensitive molecular detection and quantization are crucial for many applications including clinical diagnostics, functional proteomics, and drug discovery; however, conventional biochemical sensors cannot satisfy the stringent requirements, and this has resulted in a long-standing dilemma regarding sensitivity improvement. To this end, we have developed an ultrasensitive relay-type nanomechanical sensor based on a magneto lever. By establishing the link between very weak molecular interaction and five orders of magnitude larger magnetic force, analytes at ultratrace level can produce a clearly observable mechanical response. Initially, proof-of-concept studies showed an improved detection limit up to five orders of magnitude when employing the magneto lever, as compared with direct detection using probe alone. In this study, we subsequently demonstrated that the relay-type sensing mode was universal in application ranging from micromolecule to macromolecule detection, which can be easily extended to detect enzymes, DNA, proteins, cells, viruses, bacteria, chemicals, etc. Importantly, we found that, sensitivity was no longer subject to probe affinity when the magneto lever was sufficiently high, theoretically, even reaching single-molecule resolution. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material (experimental section) is available in the online version of this article at 10.1007/s12274-022-5049-0. Tsinghua University Press 2022-11-14 2023 /pmc/articles/PMC9661467/ /pubmed/36405983 http://dx.doi.org/10.1007/s12274-022-5049-0 Text en © Tsinghua University Press 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Article
Rao, Depeng
Yan, Tianhao
Qiao, Zihan
Wang, Yu
Peng, Yongpei
Tu, Han
Wu, Shangquan
Zhang, Qingchuan
Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
title Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
title_full Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
title_fullStr Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
title_full_unstemmed Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
title_short Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
title_sort relay-type sensing mode: a strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661467/
https://www.ncbi.nlm.nih.gov/pubmed/36405983
http://dx.doi.org/10.1007/s12274-022-5049-0
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