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Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture
Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be genetically modified due to limitations of the current gene delivery systems. Even for those that can be genetically modified, the modification involves a tedious and costly tissue culture process. Here, we describe...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661722/ https://www.ncbi.nlm.nih.gov/pubmed/36387605 http://dx.doi.org/10.1016/j.xinn.2022.100345 |
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author | Cao, Xuesong Xie, Hongtao Song, Minglei Lu, Jinghua Ma, Ping Huang, Boyu Wang, Mugui Tian, Yifu Chen, Fan Peng, Jun Lang, Zhaobo Li, Guofu Zhu, Jian-Kang |
author_facet | Cao, Xuesong Xie, Hongtao Song, Minglei Lu, Jinghua Ma, Ping Huang, Boyu Wang, Mugui Tian, Yifu Chen, Fan Peng, Jun Lang, Zhaobo Li, Guofu Zhu, Jian-Kang |
author_sort | Cao, Xuesong |
collection | PubMed |
description | Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be genetically modified due to limitations of the current gene delivery systems. Even for those that can be genetically modified, the modification involves a tedious and costly tissue culture process. Here, we describe an extremely simple cut–dip–budding (CDB) delivery system, which uses Agrobacterium rhizogene to inoculate explants, generating transformed roots that produce transformed buds due to root suckering. We have successfully used CDB to achieve the heritable transformation of plant species in multiple plant families, including two herbaceous plants (Taraxacum kok-saghyz and Coronilla varia), a tuberous root plant (sweet potato), and three woody plant species (Ailanthus altissima, Aralia elata, and Clerodendrum chinense). These plants have previously been difficult or impossible to transform, but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol, under non-sterile conditions and without the need for tissue culture. Our work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method. |
format | Online Article Text |
id | pubmed-9661722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96617222022-11-15 Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture Cao, Xuesong Xie, Hongtao Song, Minglei Lu, Jinghua Ma, Ping Huang, Boyu Wang, Mugui Tian, Yifu Chen, Fan Peng, Jun Lang, Zhaobo Li, Guofu Zhu, Jian-Kang Innovation (Camb) Report Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be genetically modified due to limitations of the current gene delivery systems. Even for those that can be genetically modified, the modification involves a tedious and costly tissue culture process. Here, we describe an extremely simple cut–dip–budding (CDB) delivery system, which uses Agrobacterium rhizogene to inoculate explants, generating transformed roots that produce transformed buds due to root suckering. We have successfully used CDB to achieve the heritable transformation of plant species in multiple plant families, including two herbaceous plants (Taraxacum kok-saghyz and Coronilla varia), a tuberous root plant (sweet potato), and three woody plant species (Ailanthus altissima, Aralia elata, and Clerodendrum chinense). These plants have previously been difficult or impossible to transform, but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol, under non-sterile conditions and without the need for tissue culture. Our work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method. Elsevier 2022-10-25 /pmc/articles/PMC9661722/ /pubmed/36387605 http://dx.doi.org/10.1016/j.xinn.2022.100345 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Report Cao, Xuesong Xie, Hongtao Song, Minglei Lu, Jinghua Ma, Ping Huang, Boyu Wang, Mugui Tian, Yifu Chen, Fan Peng, Jun Lang, Zhaobo Li, Guofu Zhu, Jian-Kang Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
title | Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
title_full | Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
title_fullStr | Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
title_full_unstemmed | Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
title_short | Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
title_sort | cut–dip–budding delivery system enables genetic modifications in plants without tissue culture |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661722/ https://www.ncbi.nlm.nih.gov/pubmed/36387605 http://dx.doi.org/10.1016/j.xinn.2022.100345 |
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