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Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages
Histidine is an essential amino acid for broiler chickens and a precursor for the dipeptides carnosine and anserine, but little information is available about its metabolism in modern, fast-growing broilers. We used untargeted metabolomics to investigate the metabolic changes caused by the use of di...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662743/ https://www.ncbi.nlm.nih.gov/pubmed/36374928 http://dx.doi.org/10.1371/journal.pone.0277476 |
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author | Lackner, Julia Hess, Vincent Marx, Achim Hosseini-Ghaffari, Morteza Sauerwein, Helga |
author_facet | Lackner, Julia Hess, Vincent Marx, Achim Hosseini-Ghaffari, Morteza Sauerwein, Helga |
author_sort | Lackner, Julia |
collection | PubMed |
description | Histidine is an essential amino acid for broiler chickens and a precursor for the dipeptides carnosine and anserine, but little information is available about its metabolism in modern, fast-growing broilers. We used untargeted metabolomics to investigate the metabolic changes caused by the use of different standardized ileal digestible His:Lys ratios in broiler diets with and without β-alanine supplementation. A total of 2204 broilers were randomly divided into 96 pens of 23 birds each. The pens were divided into 16 blocks, each containing one pen for all six feeding groups (total of 16 pens per group). These feeding groups were fed three different His:Lys ratios (0.44, 0.54, and 0.64, respectively) without and with a combination of 0.5% β-alanine supplementation. Five randomly selected chickens of one single randomly selected pen per feeding group were slaughtered on day 35 or 54, blood was collected from the neck vessel, and plasma was used for untargeted metabolomic analysis. Here we show that up to 56.0% of all metabolites analyzed were altered by age, whereas only 1.8% of metabolites were affected by the His:Lys ratio in the diet, and 1.5% by β-alanine supplementation. Two-factor analysis and metabolic pathway analysis showed no interaction between the His:Lys ratio and β-alanine supplementation. The effect of the His:Lys ratio in the diet was limited to histidine metabolism with a greater change in formiminoglutamate concentration. Supplementation of β-alanine showed changes in metabolites of several metabolic pathways; increased concentrations of 3-aminoisobutyrate showed the only direct relationship to β-alanine metabolism. The supplementation of β-alanine indicated few effects on histidine metabolism. These results suggest that the supplements used had limited effects or interactions on both His and β-alanine metabolism. In contrast, the birds’ age has the strongest influence on the metabolome. |
format | Online Article Text |
id | pubmed-9662743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96627432022-11-15 Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages Lackner, Julia Hess, Vincent Marx, Achim Hosseini-Ghaffari, Morteza Sauerwein, Helga PLoS One Research Article Histidine is an essential amino acid for broiler chickens and a precursor for the dipeptides carnosine and anserine, but little information is available about its metabolism in modern, fast-growing broilers. We used untargeted metabolomics to investigate the metabolic changes caused by the use of different standardized ileal digestible His:Lys ratios in broiler diets with and without β-alanine supplementation. A total of 2204 broilers were randomly divided into 96 pens of 23 birds each. The pens were divided into 16 blocks, each containing one pen for all six feeding groups (total of 16 pens per group). These feeding groups were fed three different His:Lys ratios (0.44, 0.54, and 0.64, respectively) without and with a combination of 0.5% β-alanine supplementation. Five randomly selected chickens of one single randomly selected pen per feeding group were slaughtered on day 35 or 54, blood was collected from the neck vessel, and plasma was used for untargeted metabolomic analysis. Here we show that up to 56.0% of all metabolites analyzed were altered by age, whereas only 1.8% of metabolites were affected by the His:Lys ratio in the diet, and 1.5% by β-alanine supplementation. Two-factor analysis and metabolic pathway analysis showed no interaction between the His:Lys ratio and β-alanine supplementation. The effect of the His:Lys ratio in the diet was limited to histidine metabolism with a greater change in formiminoglutamate concentration. Supplementation of β-alanine showed changes in metabolites of several metabolic pathways; increased concentrations of 3-aminoisobutyrate showed the only direct relationship to β-alanine metabolism. The supplementation of β-alanine indicated few effects on histidine metabolism. These results suggest that the supplements used had limited effects or interactions on both His and β-alanine metabolism. In contrast, the birds’ age has the strongest influence on the metabolome. Public Library of Science 2022-11-14 /pmc/articles/PMC9662743/ /pubmed/36374928 http://dx.doi.org/10.1371/journal.pone.0277476 Text en © 2022 Lackner et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lackner, Julia Hess, Vincent Marx, Achim Hosseini-Ghaffari, Morteza Sauerwein, Helga Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
title | Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
title_full | Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
title_fullStr | Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
title_full_unstemmed | Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
title_short | Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
title_sort | effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662743/ https://www.ncbi.nlm.nih.gov/pubmed/36374928 http://dx.doi.org/10.1371/journal.pone.0277476 |
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