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Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction
Intrauterine growth restriction (IUGR) in humans often manifests as poor growth and delayed intellectual development, whereas in domestic animals it results in increased mortality. As a novel epigenetic regulatory molecule, tRNA-derived small RNAs (tsRNAs) have been reported to be involved in many b...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662792/ https://www.ncbi.nlm.nih.gov/pubmed/36388094 http://dx.doi.org/10.3389/fphys.2022.962278 |
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author | Gan, Mailin Ma, Jianfeng Chen, Lei Zhang, Shunhua Niu, Lili Zhao, Ye Li, Xuewei Pan, Hongmei Zhu, Li Shen, Linyuan |
author_facet | Gan, Mailin Ma, Jianfeng Chen, Lei Zhang, Shunhua Niu, Lili Zhao, Ye Li, Xuewei Pan, Hongmei Zhu, Li Shen, Linyuan |
author_sort | Gan, Mailin |
collection | PubMed |
description | Intrauterine growth restriction (IUGR) in humans often manifests as poor growth and delayed intellectual development, whereas in domestic animals it results in increased mortality. As a novel epigenetic regulatory molecule, tRNA-derived small RNAs (tsRNAs) have been reported to be involved in many biological processes. In this study, pigs (35d) were used as a model to characterize tsRNAs by sequencing in normal and IUGR porcine skeletal muscle. A total of 586 tsRNAs were identified, of which 103 were specifically expressed in normal-size pigs and 38 were specifically expressed in IUGR pigs. The tsRNAs formed by splicing before the 5′ end anti codon of mature tRNA (tRF-5c) accounted for over 90% of tsRNAs, which were significantly enriched in IUGR pigs than in normal-size pigs. Enriched pathways of differentially expressed tsRNAs target genes mainly included metabolic pathways, Rap1 signaling pathway, endocytosis, mTOR signaling pathway, and AMPK signaling pathway. Regulatory network analysis of target genes revealed that IGF1 was one of the most important molecules of regulatory nodes in IUGR and normal porcine skeletal muscle. In addition, IGF1 was found to be one of the target genes of tRF-Glu-TTC-047, which is a highly expressed tsRNA in IUGR pigs. The findings described herein uncover the role of tsRNAs in IUGR porcine skeletal muscle development, thus providing insights into the prevention and treatment of IUGR in mammals. |
format | Online Article Text |
id | pubmed-9662792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96627922022-11-15 Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction Gan, Mailin Ma, Jianfeng Chen, Lei Zhang, Shunhua Niu, Lili Zhao, Ye Li, Xuewei Pan, Hongmei Zhu, Li Shen, Linyuan Front Physiol Physiology Intrauterine growth restriction (IUGR) in humans often manifests as poor growth and delayed intellectual development, whereas in domestic animals it results in increased mortality. As a novel epigenetic regulatory molecule, tRNA-derived small RNAs (tsRNAs) have been reported to be involved in many biological processes. In this study, pigs (35d) were used as a model to characterize tsRNAs by sequencing in normal and IUGR porcine skeletal muscle. A total of 586 tsRNAs were identified, of which 103 were specifically expressed in normal-size pigs and 38 were specifically expressed in IUGR pigs. The tsRNAs formed by splicing before the 5′ end anti codon of mature tRNA (tRF-5c) accounted for over 90% of tsRNAs, which were significantly enriched in IUGR pigs than in normal-size pigs. Enriched pathways of differentially expressed tsRNAs target genes mainly included metabolic pathways, Rap1 signaling pathway, endocytosis, mTOR signaling pathway, and AMPK signaling pathway. Regulatory network analysis of target genes revealed that IGF1 was one of the most important molecules of regulatory nodes in IUGR and normal porcine skeletal muscle. In addition, IGF1 was found to be one of the target genes of tRF-Glu-TTC-047, which is a highly expressed tsRNA in IUGR pigs. The findings described herein uncover the role of tsRNAs in IUGR porcine skeletal muscle development, thus providing insights into the prevention and treatment of IUGR in mammals. Frontiers Media S.A. 2022-10-31 /pmc/articles/PMC9662792/ /pubmed/36388094 http://dx.doi.org/10.3389/fphys.2022.962278 Text en Copyright © 2022 Gan, Ma, Chen, Zhang, Niu, Zhao, Li, Pan, Zhu and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Gan, Mailin Ma, Jianfeng Chen, Lei Zhang, Shunhua Niu, Lili Zhao, Ye Li, Xuewei Pan, Hongmei Zhu, Li Shen, Linyuan Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
title | Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
title_full | Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
title_fullStr | Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
title_full_unstemmed | Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
title_short | Identification of tRNA-derived small RNAs and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
title_sort | identification of trna-derived small rnas and their potential roles in porcine skeletal muscle with intrauterine growth restriction |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662792/ https://www.ncbi.nlm.nih.gov/pubmed/36388094 http://dx.doi.org/10.3389/fphys.2022.962278 |
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