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YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response
The conjugative pilus expression (Cpx) stress response system can sense cell envelope stressors, such as misfolded proteins, and upregulate proteases to modify or degrade damaged proteins. YihE is a protein kinase implicated in the Cpx system, and Rho is a transcription termination factor in prokary...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663540/ https://www.ncbi.nlm.nih.gov/pubmed/36387024 http://dx.doi.org/10.1016/j.isci.2022.105483 |
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author | Wang, Biying Pei, Hairun Lu, Zhifang Xu, Yingying Han, Shengnan Jia, Zongchao Zheng, Jimin |
author_facet | Wang, Biying Pei, Hairun Lu, Zhifang Xu, Yingying Han, Shengnan Jia, Zongchao Zheng, Jimin |
author_sort | Wang, Biying |
collection | PubMed |
description | The conjugative pilus expression (Cpx) stress response system can sense cell envelope stressors, such as misfolded proteins, and upregulate proteases to modify or degrade damaged proteins. YihE is a protein kinase implicated in the Cpx system, and Rho is a transcription termination factor in prokaryotes, but no functional connection between YihE and Rho has been reported. Here, we found that YihE can interact with Rho to form a binary complex with a stoichiometric ratio of 6:1 (Rho:YihE). A low resolution of Rho crystal structure in the presence of YihE was determined. YihE overexpression helped lessen the aberrant effects caused by Rho overexpression, including long cell morphology and other Rho-mediated phenotypes. Overall, YihE is a Rho binding partner that acts as a Rho antagonist in the Cpx stress. YihE binding to Rho would compete RNA from binding to Rho, thereby helping bacteria cope with stress through the regulation of Rho-dependent transcription termination. |
format | Online Article Text |
id | pubmed-9663540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96635402022-11-15 YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response Wang, Biying Pei, Hairun Lu, Zhifang Xu, Yingying Han, Shengnan Jia, Zongchao Zheng, Jimin iScience Article The conjugative pilus expression (Cpx) stress response system can sense cell envelope stressors, such as misfolded proteins, and upregulate proteases to modify or degrade damaged proteins. YihE is a protein kinase implicated in the Cpx system, and Rho is a transcription termination factor in prokaryotes, but no functional connection between YihE and Rho has been reported. Here, we found that YihE can interact with Rho to form a binary complex with a stoichiometric ratio of 6:1 (Rho:YihE). A low resolution of Rho crystal structure in the presence of YihE was determined. YihE overexpression helped lessen the aberrant effects caused by Rho overexpression, including long cell morphology and other Rho-mediated phenotypes. Overall, YihE is a Rho binding partner that acts as a Rho antagonist in the Cpx stress. YihE binding to Rho would compete RNA from binding to Rho, thereby helping bacteria cope with stress through the regulation of Rho-dependent transcription termination. Elsevier 2022-11-02 /pmc/articles/PMC9663540/ /pubmed/36387024 http://dx.doi.org/10.1016/j.isci.2022.105483 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wang, Biying Pei, Hairun Lu, Zhifang Xu, Yingying Han, Shengnan Jia, Zongchao Zheng, Jimin YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response |
title | YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response |
title_full | YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response |
title_fullStr | YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response |
title_full_unstemmed | YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response |
title_short | YihE is a novel binding partner of Rho and regulates Rho-dependent transcription termination in the Cpx stress response |
title_sort | yihe is a novel binding partner of rho and regulates rho-dependent transcription termination in the cpx stress response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663540/ https://www.ncbi.nlm.nih.gov/pubmed/36387024 http://dx.doi.org/10.1016/j.isci.2022.105483 |
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