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Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study

Adaptation during the domestication from wolves (Canis lupus) to dogs (Canis lupus familiaris) is a debated ecological topic. Changes in food and environment are major divergences in the domestication of dogs. Gut microbes play an important role in animal adaptation to the food and environmental cha...

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Autores principales: Chen, Lei, Sun, Mengyao, Xu, Di, Gao, Zenghao, Shi, Yuying, Wang, Shen, Zhou, Yiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663663/
https://www.ncbi.nlm.nih.gov/pubmed/36386659
http://dx.doi.org/10.3389/fmicb.2022.1027188
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author Chen, Lei
Sun, Mengyao
Xu, Di
Gao, Zenghao
Shi, Yuying
Wang, Shen
Zhou, Yiping
author_facet Chen, Lei
Sun, Mengyao
Xu, Di
Gao, Zenghao
Shi, Yuying
Wang, Shen
Zhou, Yiping
author_sort Chen, Lei
collection PubMed
description Adaptation during the domestication from wolves (Canis lupus) to dogs (Canis lupus familiaris) is a debated ecological topic. Changes in food and environment are major divergences in the domestication of dogs. Gut microbes play an important role in animal adaptation to the food and environmental changes. In this study, shotgun sequencing was performed to compare the species diversity and functional diversity of gut microbes in wild wolves (group CLW, n = 3), captive wolves (group CLC, n = 4), and domestic dogs (group CLF, n = 4). The results found that Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria and Actinobacteria were the most abundant phyla and Bacteroides, Fusobacterium, Prevotella, Megamonas, Paraprevotella, Faecalibacterium, Clostridium were the most abundant genera in the gut of wolves and dogs. Groups CLW, CLC and CLF have shown significant difference in gut microbial species diversity and functional diversity. Bacteroides, Fusobacterium and Faecalibacterium were most abundant genera in groups CLW, CLC and CLF, respectively. Their abundance varied significantly among groups. Compared to the wild wolves, the intestinal microbiol genes of domestic dogs were significantly enriched in the carbohydrate metabolism pathway of KEGG database. One hundred and seventy-seven enzymes were detected with significantly higher abundance in group CLF than that in group CLW, and 49 enzymes showed extremely significant higher abundance in group CLF than that in group CLW (q < 0.01) base on the function abundance annotated in CAZy database. It is noteworthy that there were also significant differences in the abundance of 140 enzymes between groups CLC and CLW (q < 0.05). Clustering analysis based on both the species and the function abundance of intestinal microbiota all found that groups CLC and CLF clustered into one branch, while samples from group CLW clustered into the other branch. This result suggests that captive wolves are more similar to domestic dogs than wild wolves in both species composition and function composition of intestinal microbiota.
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spelling pubmed-96636632022-11-15 Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study Chen, Lei Sun, Mengyao Xu, Di Gao, Zenghao Shi, Yuying Wang, Shen Zhou, Yiping Front Microbiol Microbiology Adaptation during the domestication from wolves (Canis lupus) to dogs (Canis lupus familiaris) is a debated ecological topic. Changes in food and environment are major divergences in the domestication of dogs. Gut microbes play an important role in animal adaptation to the food and environmental changes. In this study, shotgun sequencing was performed to compare the species diversity and functional diversity of gut microbes in wild wolves (group CLW, n = 3), captive wolves (group CLC, n = 4), and domestic dogs (group CLF, n = 4). The results found that Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria and Actinobacteria were the most abundant phyla and Bacteroides, Fusobacterium, Prevotella, Megamonas, Paraprevotella, Faecalibacterium, Clostridium were the most abundant genera in the gut of wolves and dogs. Groups CLW, CLC and CLF have shown significant difference in gut microbial species diversity and functional diversity. Bacteroides, Fusobacterium and Faecalibacterium were most abundant genera in groups CLW, CLC and CLF, respectively. Their abundance varied significantly among groups. Compared to the wild wolves, the intestinal microbiol genes of domestic dogs were significantly enriched in the carbohydrate metabolism pathway of KEGG database. One hundred and seventy-seven enzymes were detected with significantly higher abundance in group CLF than that in group CLW, and 49 enzymes showed extremely significant higher abundance in group CLF than that in group CLW (q < 0.01) base on the function abundance annotated in CAZy database. It is noteworthy that there were also significant differences in the abundance of 140 enzymes between groups CLC and CLW (q < 0.05). Clustering analysis based on both the species and the function abundance of intestinal microbiota all found that groups CLC and CLF clustered into one branch, while samples from group CLW clustered into the other branch. This result suggests that captive wolves are more similar to domestic dogs than wild wolves in both species composition and function composition of intestinal microbiota. Frontiers Media S.A. 2022-11-01 /pmc/articles/PMC9663663/ /pubmed/36386659 http://dx.doi.org/10.3389/fmicb.2022.1027188 Text en Copyright © 2022 Chen, Sun, Xu, Gao, Shi, Wang and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chen, Lei
Sun, Mengyao
Xu, Di
Gao, Zenghao
Shi, Yuying
Wang, Shen
Zhou, Yiping
Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
title Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
title_full Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
title_fullStr Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
title_full_unstemmed Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
title_short Gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
title_sort gut microbiome of captive wolves is more similar to domestic dogs than wild wolves indicated by metagenomics study
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663663/
https://www.ncbi.nlm.nih.gov/pubmed/36386659
http://dx.doi.org/10.3389/fmicb.2022.1027188
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