Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from periphe...

Descripción completa

Detalles Bibliográficos
Autores principales: Weskamm, Leonie M., Dahlke, Christine, Addo, Marylyn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663734/
https://www.ncbi.nlm.nih.gov/pubmed/36595922
http://dx.doi.org/10.1016/j.xpro.2022.101902
_version_ 1784830948811997184
author Weskamm, Leonie M.
Dahlke, Christine
Addo, Marylyn M.
author_facet Weskamm, Leonie M.
Dahlke, Christine
Addo, Marylyn M.
author_sort Weskamm, Leonie M.
collection PubMed
description Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from peripheral blood mononuclear cells and characterize their isotypes and activation status. We detail steps to use fluorescently labeled antigen probes derived from the SARS-CoV-2 spike protein. These can be adapted to detect MBCs against other antigens. For complete details on the use and execution of this protocol, please refer to Weskamm et al. (2022).(1)
format Online
Article
Text
id pubmed-9663734
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-96637342022-11-14 Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens Weskamm, Leonie M. Dahlke, Christine Addo, Marylyn M. STAR Protoc Protocol Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from peripheral blood mononuclear cells and characterize their isotypes and activation status. We detail steps to use fluorescently labeled antigen probes derived from the SARS-CoV-2 spike protein. These can be adapted to detect MBCs against other antigens. For complete details on the use and execution of this protocol, please refer to Weskamm et al. (2022).(1) Elsevier 2022-11-15 /pmc/articles/PMC9663734/ /pubmed/36595922 http://dx.doi.org/10.1016/j.xpro.2022.101902 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Weskamm, Leonie M.
Dahlke, Christine
Addo, Marylyn M.
Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
title Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
title_full Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
title_fullStr Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
title_full_unstemmed Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
title_short Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens
title_sort flow cytometric protocol to characterize human memory b cells directed against sars-cov-2 spike protein antigens
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663734/
https://www.ncbi.nlm.nih.gov/pubmed/36595922
http://dx.doi.org/10.1016/j.xpro.2022.101902
work_keys_str_mv AT weskammleoniem flowcytometricprotocoltocharacterizehumanmemorybcellsdirectedagainstsarscov2spikeproteinantigens
AT dahlkechristine flowcytometricprotocoltocharacterizehumanmemorybcellsdirectedagainstsarscov2spikeproteinantigens
AT addomarylynm flowcytometricprotocoltocharacterizehumanmemorybcellsdirectedagainstsarscov2spikeproteinantigens

Ejemplares similares