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Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain

BACKGROUND: The cerebellum is involved in hyperactivity, fear, and anxiety disorders that could be induced by whole-brain irradiation (WBI). However, whether cerebellar irradiation alone (CIA) could induce these disorders is unknown. We investigated the effect of CIA in an animal model. METHODS: Ele...

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Autores principales: Wang, Yafeng, Xie, Cuicui, Xu, Yiran, Zhang, Yaodong, Zhu, Changlian, Zhou, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663786/
https://www.ncbi.nlm.nih.gov/pubmed/36376609
http://dx.doi.org/10.1186/s41747-022-00307-8
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author Wang, Yafeng
Xie, Cuicui
Xu, Yiran
Zhang, Yaodong
Zhu, Changlian
Zhou, Kai
author_facet Wang, Yafeng
Xie, Cuicui
Xu, Yiran
Zhang, Yaodong
Zhu, Changlian
Zhou, Kai
author_sort Wang, Yafeng
collection PubMed
description BACKGROUND: The cerebellum is involved in hyperactivity, fear, and anxiety disorders that could be induced by whole-brain irradiation (WBI). However, whether cerebellar irradiation alone (CIA) could induce these disorders is unknown. We investigated the effect of CIA in an animal model. METHODS: Eleven-day-old rat pups underwent a single 3-Gy dose of either WBI (n = 28) or CIA (n = 20), while 34 rat pups were sham-irradiated (controls). Cell death was evaluated in the subgranular zone of the hippocampus by counting pyknotic cells after haematoxylin/eosin staining at 6 h after irradiation for 10, 8, and 9 pups, respectively. Behavioural changes were evaluated via open-field test at 6 weeks for 18, 12, and 25 pups, respectively. Unpaired two-tailed t-test and one-way and two-way repeated ANOVA were used. RESULTS: Massive cell death in cerebellar external granular layer was detected at 6 h after CIA (1,419 ± 211 mm, mean ± S.E.M. versus controls (68 ± 12 mm) (p < 0.001)), while no significant difference between CIA (1,419 ± 211 mm) versus WBI (1,433 ± 107 mm) (p = 0.955) was found. At open-field behavioural test, running distance, activity, wall distance, middle zone visit times, and duration were higher for WBI versus controls (p < 0.010), but no difference between CIA and controls was found (p > 0.05). CONCLUSIONS: Although the cerebellum is involved in hyperactivity, fear, and anxiety disorders, CIA did not induce these disorders, indicating that WBI-induced cerebellar injury does not directly cause these behavioural abnormalities after WBI. Thus, targeting the cerebellum alone may not be enough to rescue or reduce these behavioural abnormalities after WBI.
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spelling pubmed-96637862022-11-15 Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain Wang, Yafeng Xie, Cuicui Xu, Yiran Zhang, Yaodong Zhu, Changlian Zhou, Kai Eur Radiol Exp Original Article BACKGROUND: The cerebellum is involved in hyperactivity, fear, and anxiety disorders that could be induced by whole-brain irradiation (WBI). However, whether cerebellar irradiation alone (CIA) could induce these disorders is unknown. We investigated the effect of CIA in an animal model. METHODS: Eleven-day-old rat pups underwent a single 3-Gy dose of either WBI (n = 28) or CIA (n = 20), while 34 rat pups were sham-irradiated (controls). Cell death was evaluated in the subgranular zone of the hippocampus by counting pyknotic cells after haematoxylin/eosin staining at 6 h after irradiation for 10, 8, and 9 pups, respectively. Behavioural changes were evaluated via open-field test at 6 weeks for 18, 12, and 25 pups, respectively. Unpaired two-tailed t-test and one-way and two-way repeated ANOVA were used. RESULTS: Massive cell death in cerebellar external granular layer was detected at 6 h after CIA (1,419 ± 211 mm, mean ± S.E.M. versus controls (68 ± 12 mm) (p < 0.001)), while no significant difference between CIA (1,419 ± 211 mm) versus WBI (1,433 ± 107 mm) (p = 0.955) was found. At open-field behavioural test, running distance, activity, wall distance, middle zone visit times, and duration were higher for WBI versus controls (p < 0.010), but no difference between CIA and controls was found (p > 0.05). CONCLUSIONS: Although the cerebellum is involved in hyperactivity, fear, and anxiety disorders, CIA did not induce these disorders, indicating that WBI-induced cerebellar injury does not directly cause these behavioural abnormalities after WBI. Thus, targeting the cerebellum alone may not be enough to rescue or reduce these behavioural abnormalities after WBI. Springer Vienna 2022-11-15 /pmc/articles/PMC9663786/ /pubmed/36376609 http://dx.doi.org/10.1186/s41747-022-00307-8 Text en © The Author(s) under exclusive licence to European Society of Radiology 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Wang, Yafeng
Xie, Cuicui
Xu, Yiran
Zhang, Yaodong
Zhu, Changlian
Zhou, Kai
Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
title Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
title_full Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
title_fullStr Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
title_full_unstemmed Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
title_short Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
title_sort cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663786/
https://www.ncbi.nlm.nih.gov/pubmed/36376609
http://dx.doi.org/10.1186/s41747-022-00307-8
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