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Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.

The present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such...

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Autores principales: Shrestha, Sarita, Chio, Chonlong, Khatiwada, Janak Raj, Kognou, Aristide Laurel Mokale, Qin, Wensheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663924/
https://www.ncbi.nlm.nih.gov/pubmed/36386650
http://dx.doi.org/10.3389/fmicb.2022.1049692
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author Shrestha, Sarita
Chio, Chonlong
Khatiwada, Janak Raj
Kognou, Aristide Laurel Mokale
Qin, Wensheng
author_facet Shrestha, Sarita
Chio, Chonlong
Khatiwada, Janak Raj
Kognou, Aristide Laurel Mokale
Qin, Wensheng
author_sort Shrestha, Sarita
collection PubMed
description The present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such as pectinase, polygalacturonase, xylanase, and cellulase by using various agro-wastes as low-cost substrates. The orange peel was observed to be the best substrate among the agro-wastes used for maximum multi-enzymes (pectinase, polygalacturonase, xylanase, and cellulase). However, the bacterium demonstrated its capability to produce different enzymes according to the different substrates/agro-wastes used. The Plackett–Burman design was used to determine the essential influencing factors, while the Box Behnken design response surface methodology was for optimizing cultural conditions. At their optimal conditions (40°C incubation temperature, 24 h of incubation period, 1% w/v orange peel, and 2% v/v inoculum volume), the bacterium exhibited the maximum pectinase (9.49 ± 1.25 U/ml) and xylanase (16.27 ± 0.52 U/ml) activities. Furthermore, the study explored the ability of the bacterium to produce bacterial lipids and observed about 25% bacterial lipid content on a dry weight basis. Therefore, the bacterium is a good candidate for producing important multi-enzymes and subsequent agro-waste degradation controlling the environment, and facilitating waste management. Also, the bacterium can be a potential feedstock in producing renewable biofuel.
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spelling pubmed-96639242022-11-15 Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. Shrestha, Sarita Chio, Chonlong Khatiwada, Janak Raj Kognou, Aristide Laurel Mokale Qin, Wensheng Front Microbiol Microbiology The present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such as pectinase, polygalacturonase, xylanase, and cellulase by using various agro-wastes as low-cost substrates. The orange peel was observed to be the best substrate among the agro-wastes used for maximum multi-enzymes (pectinase, polygalacturonase, xylanase, and cellulase). However, the bacterium demonstrated its capability to produce different enzymes according to the different substrates/agro-wastes used. The Plackett–Burman design was used to determine the essential influencing factors, while the Box Behnken design response surface methodology was for optimizing cultural conditions. At their optimal conditions (40°C incubation temperature, 24 h of incubation period, 1% w/v orange peel, and 2% v/v inoculum volume), the bacterium exhibited the maximum pectinase (9.49 ± 1.25 U/ml) and xylanase (16.27 ± 0.52 U/ml) activities. Furthermore, the study explored the ability of the bacterium to produce bacterial lipids and observed about 25% bacterial lipid content on a dry weight basis. Therefore, the bacterium is a good candidate for producing important multi-enzymes and subsequent agro-waste degradation controlling the environment, and facilitating waste management. Also, the bacterium can be a potential feedstock in producing renewable biofuel. Frontiers Media S.A. 2022-11-01 /pmc/articles/PMC9663924/ /pubmed/36386650 http://dx.doi.org/10.3389/fmicb.2022.1049692 Text en Copyright © 2022 Shrestha, Chio, Khatiwada, Kognou and Qin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Shrestha, Sarita
Chio, Chonlong
Khatiwada, Janak Raj
Kognou, Aristide Laurel Mokale
Qin, Wensheng
Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
title Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
title_full Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
title_fullStr Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
title_full_unstemmed Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
title_short Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
title_sort optimization of multiple enzymes production by fermentation using lipid-producing bacillus sp.
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663924/
https://www.ncbi.nlm.nih.gov/pubmed/36386650
http://dx.doi.org/10.3389/fmicb.2022.1049692
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