Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography

Exosomes are a class of extracellular vesicles that play a role in intercellular signaling under diverse contexts. Here, we describe a protocol that has been optimized for the isolation and characterization of exosomes from a Drosophila melanogaster cell line using size exclusion chromatography (SEC...

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Detalles Bibliográficos
Autores principales: Pandey, Manish, Chawla, Geetanjali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663981/
https://www.ncbi.nlm.nih.gov/pubmed/36386877
http://dx.doi.org/10.1016/j.xpro.2022.101834
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author Pandey, Manish
Chawla, Geetanjali
author_facet Pandey, Manish
Chawla, Geetanjali
author_sort Pandey, Manish
collection PubMed
description Exosomes are a class of extracellular vesicles that play a role in intercellular signaling under diverse contexts. Here, we describe a protocol that has been optimized for the isolation and characterization of exosomes from a Drosophila melanogaster cell line using size exclusion chromatography (SEC). The specific focus of this protocol was to examine the starvation-induced exosome loading of a protein. For complete details on the use and execution of this protocol, please refer to Pandey et al. (2021).(1)
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spelling pubmed-96639812022-11-15 Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography Pandey, Manish Chawla, Geetanjali STAR Protoc Protocol Exosomes are a class of extracellular vesicles that play a role in intercellular signaling under diverse contexts. Here, we describe a protocol that has been optimized for the isolation and characterization of exosomes from a Drosophila melanogaster cell line using size exclusion chromatography (SEC). The specific focus of this protocol was to examine the starvation-induced exosome loading of a protein. For complete details on the use and execution of this protocol, please refer to Pandey et al. (2021).(1) Elsevier 2022-11-10 /pmc/articles/PMC9663981/ /pubmed/36386877 http://dx.doi.org/10.1016/j.xpro.2022.101834 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pandey, Manish
Chawla, Geetanjali
Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography
title Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography
title_full Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography
title_fullStr Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography
title_full_unstemmed Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography
title_short Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography
title_sort purification of exosome-enriched proteins produced in a drosophila cell line by size exclusion chromatography
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9663981/
https://www.ncbi.nlm.nih.gov/pubmed/36386877
http://dx.doi.org/10.1016/j.xpro.2022.101834
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