Protocol to isolate mature thymic T cell subsets using fluorescence-activated cell sorting for assessing gene expression by RNA-seq and transcription factor binding across the genome by CUT&RUN

Here, we describe steps to isolate mature thymic T cell subsets, namely CD4 single positive (SP), CD8 SP, and invariant natural killer T (iNKT) cells starting from murine total thymocytes using fluorescence-activated cell sorting. We detail protocols to study gene expression by RNA-seq and assess bi...

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Detalles Bibliográficos
Autores principales: Theofilatos, Dimitris, Äijö, Tarmo, Tsagaratou, Ageliki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664014/
https://www.ncbi.nlm.nih.gov/pubmed/36386867
http://dx.doi.org/10.1016/j.xpro.2022.101839

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