Label-free quantitative proteomic analysis of adult Drosophila heads

LIR motif-containing proteins (LIRCPs) bind to LDS (LIR motif docking site) of Atg8-family proteins. In this protocol, we describe steps to identify Drosophila LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins fro...

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Detalles Bibliográficos
Autores principales: Zhang, Yan, Nezis, Ioannis P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664018/
https://www.ncbi.nlm.nih.gov/pubmed/36386889
http://dx.doi.org/10.1016/j.xpro.2022.101830
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author Zhang, Yan
Nezis, Ioannis P.
author_facet Zhang, Yan
Nezis, Ioannis P.
author_sort Zhang, Yan
collection PubMed
description LIR motif-containing proteins (LIRCPs) bind to LDS (LIR motif docking site) of Atg8-family proteins. In this protocol, we describe steps to identify Drosophila LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins from adult Drosophila heads, followed by liquid chromatography–mass spectrometry (LC-MS/MS) analysis. We also describe screening steps of upregulated proteins in Atg8a LDS mutants, leading to identification of novel LIRCPs in Drosophila. For complete details on the use and execution of this protocol, please refer to Rahman et al. (2022).
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spelling pubmed-96640182022-11-15 Label-free quantitative proteomic analysis of adult Drosophila heads Zhang, Yan Nezis, Ioannis P. STAR Protoc Protocol LIR motif-containing proteins (LIRCPs) bind to LDS (LIR motif docking site) of Atg8-family proteins. In this protocol, we describe steps to identify Drosophila LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins from adult Drosophila heads, followed by liquid chromatography–mass spectrometry (LC-MS/MS) analysis. We also describe screening steps of upregulated proteins in Atg8a LDS mutants, leading to identification of novel LIRCPs in Drosophila. For complete details on the use and execution of this protocol, please refer to Rahman et al. (2022). Elsevier 2022-11-11 /pmc/articles/PMC9664018/ /pubmed/36386889 http://dx.doi.org/10.1016/j.xpro.2022.101830 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Zhang, Yan
Nezis, Ioannis P.
Label-free quantitative proteomic analysis of adult Drosophila heads
title Label-free quantitative proteomic analysis of adult Drosophila heads
title_full Label-free quantitative proteomic analysis of adult Drosophila heads
title_fullStr Label-free quantitative proteomic analysis of adult Drosophila heads
title_full_unstemmed Label-free quantitative proteomic analysis of adult Drosophila heads
title_short Label-free quantitative proteomic analysis of adult Drosophila heads
title_sort label-free quantitative proteomic analysis of adult drosophila heads
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664018/
https://www.ncbi.nlm.nih.gov/pubmed/36386889
http://dx.doi.org/10.1016/j.xpro.2022.101830
work_keys_str_mv AT zhangyan labelfreequantitativeproteomicanalysisofadultdrosophilaheads
AT nezisioannisp labelfreequantitativeproteomicanalysisofadultdrosophilaheads

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