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Label-free quantitative proteomic analysis of adult Drosophila heads

LIR motif-containing proteins (LIRCPs) bind to LDS (LIR motif docking site) of Atg8-family proteins. In this protocol, we describe steps to identify Drosophila LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins fro...

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Detalles Bibliográficos
Autores principales:	Zhang, Yan, Nezis, Ioannis P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664018/ https://www.ncbi.nlm.nih.gov/pubmed/36386889 http://dx.doi.org/10.1016/j.xpro.2022.101830

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