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Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica

The MADS-box gene family plays a crucial role in multiple developmental processes of plants, especially in floral organ specification and the regulation of fruit development and ripening. Juglans mandshurica is a precious fruit material whose quality and yield are determined by floral organ developm...

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Detalles Bibliográficos
Autores principales: Li, Hanxi, Li, Yuxi, Zhang, Xinxin, Cai, Kewei, Li, Yan, Wang, Qingcheng, Qu, Guanzheng, Han, Rui, Zhao, Xiyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664150/
https://www.ncbi.nlm.nih.gov/pubmed/36388573
http://dx.doi.org/10.3389/fpls.2022.1020706
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author Li, Hanxi
Li, Yuxi
Zhang, Xinxin
Cai, Kewei
Li, Yan
Wang, Qingcheng
Qu, Guanzheng
Han, Rui
Zhao, Xiyang
author_facet Li, Hanxi
Li, Yuxi
Zhang, Xinxin
Cai, Kewei
Li, Yan
Wang, Qingcheng
Qu, Guanzheng
Han, Rui
Zhao, Xiyang
author_sort Li, Hanxi
collection PubMed
description The MADS-box gene family plays a crucial role in multiple developmental processes of plants, especially in floral organ specification and the regulation of fruit development and ripening. Juglans mandshurica is a precious fruit material whose quality and yield are determined by floral organ development. The molecular mechanism of J. mandshurica female and male flower development depending on MADS-box genes remains unclear. In our study, 67 JmMADS genes were identified and unevenly distributed on 15 of 16 J. mandshurica chromosomes. These genes were divided into two types [type I (Mα, Mγ, Mδ) and type II (MIKC)]. The gene structure and motif analyses showed that most genes belonging to the same type had similar gene structures and conserved motifs. The analysis of syntenic relationships showed that MADS-box genes in J. mandshurica, J. sigillata, and J. regia exhibited the highest homology and great collinearity. Analysis of cis-acting elements showed that JmMADS gene promoter regions contained light, stress and hormone response cis-acting elements. The gene expression patterns demonstrated that 30 and 26 JmMADS genes were specifically expressed in the female and male flowers, respectively. In addition, 12 selected genes common to J. mandshurica female and male flowers were significantly upregulated at the mature stage and were used to validate the reliability of the transcriptome data using quantitative real-time PCR. This comprehensive and systematic analysis of J. mandshurica MADS-box genes lays a foundation for future studies on MADS-box gene family functions.
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spelling pubmed-96641502022-11-15 Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica Li, Hanxi Li, Yuxi Zhang, Xinxin Cai, Kewei Li, Yan Wang, Qingcheng Qu, Guanzheng Han, Rui Zhao, Xiyang Front Plant Sci Plant Science The MADS-box gene family plays a crucial role in multiple developmental processes of plants, especially in floral organ specification and the regulation of fruit development and ripening. Juglans mandshurica is a precious fruit material whose quality and yield are determined by floral organ development. The molecular mechanism of J. mandshurica female and male flower development depending on MADS-box genes remains unclear. In our study, 67 JmMADS genes were identified and unevenly distributed on 15 of 16 J. mandshurica chromosomes. These genes were divided into two types [type I (Mα, Mγ, Mδ) and type II (MIKC)]. The gene structure and motif analyses showed that most genes belonging to the same type had similar gene structures and conserved motifs. The analysis of syntenic relationships showed that MADS-box genes in J. mandshurica, J. sigillata, and J. regia exhibited the highest homology and great collinearity. Analysis of cis-acting elements showed that JmMADS gene promoter regions contained light, stress and hormone response cis-acting elements. The gene expression patterns demonstrated that 30 and 26 JmMADS genes were specifically expressed in the female and male flowers, respectively. In addition, 12 selected genes common to J. mandshurica female and male flowers were significantly upregulated at the mature stage and were used to validate the reliability of the transcriptome data using quantitative real-time PCR. This comprehensive and systematic analysis of J. mandshurica MADS-box genes lays a foundation for future studies on MADS-box gene family functions. Frontiers Media S.A. 2022-11-01 /pmc/articles/PMC9664150/ /pubmed/36388573 http://dx.doi.org/10.3389/fpls.2022.1020706 Text en Copyright © 2022 Li, Li, Zhang, Cai, Li, Wang, Qu, Han and Zhao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Hanxi
Li, Yuxi
Zhang, Xinxin
Cai, Kewei
Li, Yan
Wang, Qingcheng
Qu, Guanzheng
Han, Rui
Zhao, Xiyang
Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica
title Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica
title_full Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica
title_fullStr Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica
title_full_unstemmed Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica
title_short Genome-wide identification and expression analysis of the MADS-box gene family during female and male flower development in Juglans mandshurica
title_sort genome-wide identification and expression analysis of the mads-box gene family during female and male flower development in juglans mandshurica
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664150/
https://www.ncbi.nlm.nih.gov/pubmed/36388573
http://dx.doi.org/10.3389/fpls.2022.1020706
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