Cargando…

Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients

To study the role and mechanism of aquaporin-8 (AQP8) in placental vascular development in gestational diabetes mellitus (GDM), hematoxylin–eosin staining and immunohistochemistry were utilized to analyze the histopathological changes in placentas in GDM patients. Transwell, CCK-8, and tube formatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Shan, Yanxing, Cui, Jiawen, Kang, Xinyi, Tang, Weichun, Lu, Yiling, Gao, Ying, Chen, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664169/
https://www.ncbi.nlm.nih.gov/pubmed/36448064
http://dx.doi.org/10.1515/biol-2022-0522
_version_ 1784831045650087936
author Shan, Yanxing
Cui, Jiawen
Kang, Xinyi
Tang, Weichun
Lu, Yiling
Gao, Ying
Chen, Liping
author_facet Shan, Yanxing
Cui, Jiawen
Kang, Xinyi
Tang, Weichun
Lu, Yiling
Gao, Ying
Chen, Liping
author_sort Shan, Yanxing
collection PubMed
description To study the role and mechanism of aquaporin-8 (AQP8) in placental vascular development in gestational diabetes mellitus (GDM), hematoxylin–eosin staining and immunohistochemistry were utilized to analyze the histopathological changes in placentas in GDM patients. Transwell, CCK-8, and tube formation assays were performed to examine cell migration, proliferation, and tube formation. AQP8, vascular cell adhesion molecule 1 (VCAM-1), tumor necrosis factor alpha (TNF)-α, and vascular endothelial growth factor (VEGF)-A expression levels were investigated. Relative to the control group, the placentas in the GDM group showed morphological changes, the number of microvessels in the placental villi arterioles was significantly higher, and the area of microvessels in the arterioles of placental villi was significantly lower. The expression levels of VCAM-1, TNF-α, VEGF-A, and AQP8 in the GDM placentas and human umbilical vein endothelial cells (HUVECs) stimulated by high glucose were significantly higher than those in the control group, and AQP8 was located in placental endothelial cells. Overexpression of glucose and AQP8 inhibited tube formation, migration, and proliferation in HUVECs. High glucose levels can induce dysfunction in vascular endothelial cells and lead to pathological changes in the placental vascular structure in GDM. AQP8 overexpression in placental GDM can inhibit endothelial cell behavior, cause endothelial cell dysfunction, and further participate in the occurrence and development of GDM placental vascular lesions.
format Online
Article
Text
id pubmed-9664169
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher De Gruyter
record_format MEDLINE/PubMed
spelling pubmed-96641692022-11-28 Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients Shan, Yanxing Cui, Jiawen Kang, Xinyi Tang, Weichun Lu, Yiling Gao, Ying Chen, Liping Open Life Sci Research Article To study the role and mechanism of aquaporin-8 (AQP8) in placental vascular development in gestational diabetes mellitus (GDM), hematoxylin–eosin staining and immunohistochemistry were utilized to analyze the histopathological changes in placentas in GDM patients. Transwell, CCK-8, and tube formation assays were performed to examine cell migration, proliferation, and tube formation. AQP8, vascular cell adhesion molecule 1 (VCAM-1), tumor necrosis factor alpha (TNF)-α, and vascular endothelial growth factor (VEGF)-A expression levels were investigated. Relative to the control group, the placentas in the GDM group showed morphological changes, the number of microvessels in the placental villi arterioles was significantly higher, and the area of microvessels in the arterioles of placental villi was significantly lower. The expression levels of VCAM-1, TNF-α, VEGF-A, and AQP8 in the GDM placentas and human umbilical vein endothelial cells (HUVECs) stimulated by high glucose were significantly higher than those in the control group, and AQP8 was located in placental endothelial cells. Overexpression of glucose and AQP8 inhibited tube formation, migration, and proliferation in HUVECs. High glucose levels can induce dysfunction in vascular endothelial cells and lead to pathological changes in the placental vascular structure in GDM. AQP8 overexpression in placental GDM can inhibit endothelial cell behavior, cause endothelial cell dysfunction, and further participate in the occurrence and development of GDM placental vascular lesions. De Gruyter 2022-11-14 /pmc/articles/PMC9664169/ /pubmed/36448064 http://dx.doi.org/10.1515/biol-2022-0522 Text en © 2022 Yanxing Shan et al., published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Shan, Yanxing
Cui, Jiawen
Kang, Xinyi
Tang, Weichun
Lu, Yiling
Gao, Ying
Chen, Liping
Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
title Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
title_full Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
title_fullStr Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
title_full_unstemmed Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
title_short Aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
title_sort aquaporin-8 overexpression is involved in vascular structure and function changes in placentas of gestational diabetes mellitus patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664169/
https://www.ncbi.nlm.nih.gov/pubmed/36448064
http://dx.doi.org/10.1515/biol-2022-0522
work_keys_str_mv AT shanyanxing aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients
AT cuijiawen aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients
AT kangxinyi aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients
AT tangweichun aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients
AT luyiling aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients
AT gaoying aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients
AT chenliping aquaporin8overexpressionisinvolvedinvascularstructureandfunctionchangesinplacentasofgestationaldiabetesmellituspatients