Cargando…

Cell-intrinsic differences between human airway epithelial cells from children and adults

The airway epithelium is a protective barrier that is maintained by the self-renewal and differentiation of basal stem cells. Increasing age is a principle risk factor for chronic lung diseases, but few studies have explored age-related molecular or functional changes in the airway epithelium. We re...

Descripción completa

Detalles Bibliográficos
Autores principales: Maughan, Elizabeth F., Hynds, Robert E., Pennycuick, Adam, Nigro, Ersilia, Gowers, Kate H.C., Denais, Celine, Gómez-López, Sandra, Lazarus, Kyren A., Orr, Jessica C., Pearce, David R., Clarke, Sarah E., Lee, Dani Do Hyang, Woodall, Maximillian N.J., Masonou, Tereza, Case, Katie-Marie, Teixeira, Vitor H., Hartley, Benjamin E., Hewitt, Richard J., Al Yaghchi, Chadwan, Sandhu, Gurpreet S., Birchall, Martin A., O’Callaghan, Christopher, Smith, Claire M., De Coppi, Paolo, Butler, Colin R., Janes, Sam M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664344/
https://www.ncbi.nlm.nih.gov/pubmed/36388965
http://dx.doi.org/10.1016/j.isci.2022.105409
_version_ 1784831081872097280
author Maughan, Elizabeth F.
Hynds, Robert E.
Pennycuick, Adam
Nigro, Ersilia
Gowers, Kate H.C.
Denais, Celine
Gómez-López, Sandra
Lazarus, Kyren A.
Orr, Jessica C.
Pearce, David R.
Clarke, Sarah E.
Lee, Dani Do Hyang
Woodall, Maximillian N.J.
Masonou, Tereza
Case, Katie-Marie
Teixeira, Vitor H.
Hartley, Benjamin E.
Hewitt, Richard J.
Al Yaghchi, Chadwan
Sandhu, Gurpreet S.
Birchall, Martin A.
O’Callaghan, Christopher
Smith, Claire M.
De Coppi, Paolo
Butler, Colin R.
Janes, Sam M.
author_facet Maughan, Elizabeth F.
Hynds, Robert E.
Pennycuick, Adam
Nigro, Ersilia
Gowers, Kate H.C.
Denais, Celine
Gómez-López, Sandra
Lazarus, Kyren A.
Orr, Jessica C.
Pearce, David R.
Clarke, Sarah E.
Lee, Dani Do Hyang
Woodall, Maximillian N.J.
Masonou, Tereza
Case, Katie-Marie
Teixeira, Vitor H.
Hartley, Benjamin E.
Hewitt, Richard J.
Al Yaghchi, Chadwan
Sandhu, Gurpreet S.
Birchall, Martin A.
O’Callaghan, Christopher
Smith, Claire M.
De Coppi, Paolo
Butler, Colin R.
Janes, Sam M.
author_sort Maughan, Elizabeth F.
collection PubMed
description The airway epithelium is a protective barrier that is maintained by the self-renewal and differentiation of basal stem cells. Increasing age is a principle risk factor for chronic lung diseases, but few studies have explored age-related molecular or functional changes in the airway epithelium. We retrieved epithelial biopsies from histologically normal tracheobronchial sites from pediatric and adult donors and compared their cellular composition and gene expression profile (in laser capture-microdissected whole epithelium, fluorescence-activated cell-sorted basal cells, and basal cells in cell culture). Histologically, pediatric and adult tracheobronchial epithelium was similar in composition. We observed age-associated changes in RNA sequencing studies, including higher interferon-associated gene expression in pediatric epithelium. In cell culture, pediatric cells had higher colony formation ability, sustained in vitro growth, and outcompeted adult cells in a direct competitive proliferation assay. Our results demonstrate cell-intrinsic differences between airway epithelial cells from children and adults in both homeostatic and proliferative states.
format Online
Article
Text
id pubmed-9664344
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-96643442022-11-15 Cell-intrinsic differences between human airway epithelial cells from children and adults Maughan, Elizabeth F. Hynds, Robert E. Pennycuick, Adam Nigro, Ersilia Gowers, Kate H.C. Denais, Celine Gómez-López, Sandra Lazarus, Kyren A. Orr, Jessica C. Pearce, David R. Clarke, Sarah E. Lee, Dani Do Hyang Woodall, Maximillian N.J. Masonou, Tereza Case, Katie-Marie Teixeira, Vitor H. Hartley, Benjamin E. Hewitt, Richard J. Al Yaghchi, Chadwan Sandhu, Gurpreet S. Birchall, Martin A. O’Callaghan, Christopher Smith, Claire M. De Coppi, Paolo Butler, Colin R. Janes, Sam M. iScience Article The airway epithelium is a protective barrier that is maintained by the self-renewal and differentiation of basal stem cells. Increasing age is a principle risk factor for chronic lung diseases, but few studies have explored age-related molecular or functional changes in the airway epithelium. We retrieved epithelial biopsies from histologically normal tracheobronchial sites from pediatric and adult donors and compared their cellular composition and gene expression profile (in laser capture-microdissected whole epithelium, fluorescence-activated cell-sorted basal cells, and basal cells in cell culture). Histologically, pediatric and adult tracheobronchial epithelium was similar in composition. We observed age-associated changes in RNA sequencing studies, including higher interferon-associated gene expression in pediatric epithelium. In cell culture, pediatric cells had higher colony formation ability, sustained in vitro growth, and outcompeted adult cells in a direct competitive proliferation assay. Our results demonstrate cell-intrinsic differences between airway epithelial cells from children and adults in both homeostatic and proliferative states. Elsevier 2022-10-20 /pmc/articles/PMC9664344/ /pubmed/36388965 http://dx.doi.org/10.1016/j.isci.2022.105409 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maughan, Elizabeth F.
Hynds, Robert E.
Pennycuick, Adam
Nigro, Ersilia
Gowers, Kate H.C.
Denais, Celine
Gómez-López, Sandra
Lazarus, Kyren A.
Orr, Jessica C.
Pearce, David R.
Clarke, Sarah E.
Lee, Dani Do Hyang
Woodall, Maximillian N.J.
Masonou, Tereza
Case, Katie-Marie
Teixeira, Vitor H.
Hartley, Benjamin E.
Hewitt, Richard J.
Al Yaghchi, Chadwan
Sandhu, Gurpreet S.
Birchall, Martin A.
O’Callaghan, Christopher
Smith, Claire M.
De Coppi, Paolo
Butler, Colin R.
Janes, Sam M.
Cell-intrinsic differences between human airway epithelial cells from children and adults
title Cell-intrinsic differences between human airway epithelial cells from children and adults
title_full Cell-intrinsic differences between human airway epithelial cells from children and adults
title_fullStr Cell-intrinsic differences between human airway epithelial cells from children and adults
title_full_unstemmed Cell-intrinsic differences between human airway epithelial cells from children and adults
title_short Cell-intrinsic differences between human airway epithelial cells from children and adults
title_sort cell-intrinsic differences between human airway epithelial cells from children and adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664344/
https://www.ncbi.nlm.nih.gov/pubmed/36388965
http://dx.doi.org/10.1016/j.isci.2022.105409
work_keys_str_mv AT maughanelizabethf cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT hyndsroberte cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT pennycuickadam cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT nigroersilia cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT gowerskatehc cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT denaisceline cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT gomezlopezsandra cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT lazaruskyrena cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT orrjessicac cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT pearcedavidr cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT clarkesarahe cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT leedanidohyang cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT woodallmaximilliannj cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT masonoutereza cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT casekatiemarie cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT teixeiravitorh cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT hartleybenjamine cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT hewittrichardj cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT alyaghchichadwan cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT sandhugurpreets cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT birchallmartina cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT ocallaghanchristopher cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT smithclairem cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT decoppipaolo cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT butlercolinr cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults
AT janessamm cellintrinsicdifferencesbetweenhumanairwayepithelialcellsfromchildrenandadults