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tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity

Emerging and re-emerging respiratory viruses can spread rapidly and cause pandemics as demonstrated by the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The early human immune responses to respiratory viruses are in the nasal cavity and nasopharyngeal regions. Definin...

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Autores principales: Katanski, Christopher D., Alshammary, Hala, Watkins, Christopher P., Huang, Sihao, Gonzales-Reiche, Ana, Sordillo, Emilia Mia, van Bakel, Harm, Lolans, Karen, Simon, Viviana, Pan, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664364/
https://www.ncbi.nlm.nih.gov/pubmed/36393870
http://dx.doi.org/10.3389/fcell.2022.999351
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author Katanski, Christopher D.
Alshammary, Hala
Watkins, Christopher P.
Huang, Sihao
Gonzales-Reiche, Ana
Sordillo, Emilia Mia
van Bakel, Harm
Lolans, Karen
Simon, Viviana
Pan, Tao
author_facet Katanski, Christopher D.
Alshammary, Hala
Watkins, Christopher P.
Huang, Sihao
Gonzales-Reiche, Ana
Sordillo, Emilia Mia
van Bakel, Harm
Lolans, Karen
Simon, Viviana
Pan, Tao
author_sort Katanski, Christopher D.
collection PubMed
description Emerging and re-emerging respiratory viruses can spread rapidly and cause pandemics as demonstrated by the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The early human immune responses to respiratory viruses are in the nasal cavity and nasopharyngeal regions. Defining biomarkers of disease trajectory at the time of a positive diagnostic test would be an important tool to facilitate decisions such as initiation of antiviral treatment. We hypothesize that nasopharyngeal tRNA profiles could be used to predict Coronavirus Disease 19 (COVID-19) severity. We carried out multiplex small RNA sequencing (MSR-seq) on residual nasopharyngeal swabs to measure simultaneously full-length tRNA abundance, tRNA modifications, and tRNA fragmentation for the human tRNA response to SARS-CoV-2 infection. We identified distinct tRNA signatures associated with mild symptoms versus severe COVID-19 manifestations requiring hospitalization. These results highlight the utility of host tRNA properties as biomarkers for the clinical outcome of SARS-CoV-2.
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spelling pubmed-96643642022-11-15 tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity Katanski, Christopher D. Alshammary, Hala Watkins, Christopher P. Huang, Sihao Gonzales-Reiche, Ana Sordillo, Emilia Mia van Bakel, Harm Lolans, Karen Simon, Viviana Pan, Tao Front Cell Dev Biol Cell and Developmental Biology Emerging and re-emerging respiratory viruses can spread rapidly and cause pandemics as demonstrated by the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The early human immune responses to respiratory viruses are in the nasal cavity and nasopharyngeal regions. Defining biomarkers of disease trajectory at the time of a positive diagnostic test would be an important tool to facilitate decisions such as initiation of antiviral treatment. We hypothesize that nasopharyngeal tRNA profiles could be used to predict Coronavirus Disease 19 (COVID-19) severity. We carried out multiplex small RNA sequencing (MSR-seq) on residual nasopharyngeal swabs to measure simultaneously full-length tRNA abundance, tRNA modifications, and tRNA fragmentation for the human tRNA response to SARS-CoV-2 infection. We identified distinct tRNA signatures associated with mild symptoms versus severe COVID-19 manifestations requiring hospitalization. These results highlight the utility of host tRNA properties as biomarkers for the clinical outcome of SARS-CoV-2. Frontiers Media S.A. 2022-11-01 /pmc/articles/PMC9664364/ /pubmed/36393870 http://dx.doi.org/10.3389/fcell.2022.999351 Text en Copyright © 2022 Katanski, Alshammary, Watkins, Huang, Gonzales-Reiche, Sordillo, van Bakel, Mount Sinai PSP study group, Lolans, Simon and Pan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Katanski, Christopher D.
Alshammary, Hala
Watkins, Christopher P.
Huang, Sihao
Gonzales-Reiche, Ana
Sordillo, Emilia Mia
van Bakel, Harm
Lolans, Karen
Simon, Viviana
Pan, Tao
tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity
title tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity
title_full tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity
title_fullStr tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity
title_full_unstemmed tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity
title_short tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity
title_sort trna abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for covid-19 severity
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664364/
https://www.ncbi.nlm.nih.gov/pubmed/36393870
http://dx.doi.org/10.3389/fcell.2022.999351
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