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Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages

The sea urchin Paracentrotus lividus has been used as a model system in biology for more than a century. Over the past decades, it has been at the center of a number of studies in cell, developmental, ecological, toxicological, evolutionary, and aquaculture research. Due to this previous work, a sig...

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Autores principales: Formery, Laurent, Wakefield, Axel, Gesson, Maeva, Toisoul, Ludovic, Lhomond, Guy, Gilletta, Laurent, Lasbleiz, Régis, Schubert, Michael, Croce, Jenifer C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664562/
https://www.ncbi.nlm.nih.gov/pubmed/36393864
http://dx.doi.org/10.3389/fcell.2022.966408
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author Formery, Laurent
Wakefield, Axel
Gesson, Maeva
Toisoul, Ludovic
Lhomond, Guy
Gilletta, Laurent
Lasbleiz, Régis
Schubert, Michael
Croce, Jenifer C.
author_facet Formery, Laurent
Wakefield, Axel
Gesson, Maeva
Toisoul, Ludovic
Lhomond, Guy
Gilletta, Laurent
Lasbleiz, Régis
Schubert, Michael
Croce, Jenifer C.
author_sort Formery, Laurent
collection PubMed
description The sea urchin Paracentrotus lividus has been used as a model system in biology for more than a century. Over the past decades, it has been at the center of a number of studies in cell, developmental, ecological, toxicological, evolutionary, and aquaculture research. Due to this previous work, a significant amount of information is already available on the development of this species. However, this information is fragmented and rather incomplete. Here, we propose a comprehensive developmental atlas for this sea urchin species, describing its ontogeny from fertilization to juvenile stages. Our staging scheme includes three periods divided into 33 stages, plus 15 independent stages focused on the development of the coeloms and the adult rudiment. For each stage, we provide a thorough description based on observations made on live specimens using light microscopy, and when needed on fixed specimens using confocal microscopy. Our descriptions include, for each stage, the main anatomical characteristics related, for instance, to cell division, tissue morphogenesis, and/or organogenesis. Altogether, this work is the first of its kind providing, in a single study, a comprehensive description of the development of P. lividus embryos, larvae, and juveniles, including details on skeletogenesis, ciliogenesis, myogenesis, coelomogenesis, and formation of the adult rudiment as well as on the process of metamorphosis in live specimens. Given the renewed interest for the use of sea urchins in ecotoxicological, developmental, and evolutionary studies as well as in using marine invertebrates as alternative model systems for biomedical investigations, this study will greatly benefit the scientific community and will serve as a reference for specialists and non-specialists interested in studying sea urchins.
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spelling pubmed-96645622022-11-15 Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages Formery, Laurent Wakefield, Axel Gesson, Maeva Toisoul, Ludovic Lhomond, Guy Gilletta, Laurent Lasbleiz, Régis Schubert, Michael Croce, Jenifer C. Front Cell Dev Biol Cell and Developmental Biology The sea urchin Paracentrotus lividus has been used as a model system in biology for more than a century. Over the past decades, it has been at the center of a number of studies in cell, developmental, ecological, toxicological, evolutionary, and aquaculture research. Due to this previous work, a significant amount of information is already available on the development of this species. However, this information is fragmented and rather incomplete. Here, we propose a comprehensive developmental atlas for this sea urchin species, describing its ontogeny from fertilization to juvenile stages. Our staging scheme includes three periods divided into 33 stages, plus 15 independent stages focused on the development of the coeloms and the adult rudiment. For each stage, we provide a thorough description based on observations made on live specimens using light microscopy, and when needed on fixed specimens using confocal microscopy. Our descriptions include, for each stage, the main anatomical characteristics related, for instance, to cell division, tissue morphogenesis, and/or organogenesis. Altogether, this work is the first of its kind providing, in a single study, a comprehensive description of the development of P. lividus embryos, larvae, and juveniles, including details on skeletogenesis, ciliogenesis, myogenesis, coelomogenesis, and formation of the adult rudiment as well as on the process of metamorphosis in live specimens. Given the renewed interest for the use of sea urchins in ecotoxicological, developmental, and evolutionary studies as well as in using marine invertebrates as alternative model systems for biomedical investigations, this study will greatly benefit the scientific community and will serve as a reference for specialists and non-specialists interested in studying sea urchins. Frontiers Media S.A. 2022-10-31 /pmc/articles/PMC9664562/ /pubmed/36393864 http://dx.doi.org/10.3389/fcell.2022.966408 Text en Copyright © 2022 Formery, Wakefield, Gesson, Toisoul, Lhomond, Gilletta, Lasbleiz, Schubert and Croce. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Formery, Laurent
Wakefield, Axel
Gesson, Maeva
Toisoul, Ludovic
Lhomond, Guy
Gilletta, Laurent
Lasbleiz, Régis
Schubert, Michael
Croce, Jenifer C.
Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages
title Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages
title_full Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages
title_fullStr Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages
title_full_unstemmed Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages
title_short Developmental atlas of the indirect-developing sea urchin Paracentrotus lividus: From fertilization to juvenile stages
title_sort developmental atlas of the indirect-developing sea urchin paracentrotus lividus: from fertilization to juvenile stages
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664562/
https://www.ncbi.nlm.nih.gov/pubmed/36393864
http://dx.doi.org/10.3389/fcell.2022.966408
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