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Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI

Glioblastoma (GBM) is a malignant tumor. The long-term prognosis of the patients is poor. Therefore, it is of important clinical value to further explore the pathogenesis and look for molecular markers for early diagnosis and targeted treatment. Two expression profiling datasets [GSE50161 (GPL570 pl...

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Autores principales: Zhang, Shuyuan, Zhang, Weiwei, Wu, Bin, Xia, Liang, Li, Liwen, Jin, Kai, Zou, Yangfan, Sun, Caixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666166/
https://www.ncbi.nlm.nih.gov/pubmed/36397358
http://dx.doi.org/10.1097/MD.0000000000031418
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author Zhang, Shuyuan
Zhang, Weiwei
Wu, Bin
Xia, Liang
Li, Liwen
Jin, Kai
Zou, Yangfan
Sun, Caixing
author_facet Zhang, Shuyuan
Zhang, Weiwei
Wu, Bin
Xia, Liang
Li, Liwen
Jin, Kai
Zou, Yangfan
Sun, Caixing
author_sort Zhang, Shuyuan
collection PubMed
description Glioblastoma (GBM) is a malignant tumor. The long-term prognosis of the patients is poor. Therefore, it is of important clinical value to further explore the pathogenesis and look for molecular markers for early diagnosis and targeted treatment. Two expression profiling datasets [GSE50161 (GPL570 platform), GSE116520 (GPL10558 platform)] were respectively downloaded from the gene expression omnibus database. Volcano diagrams show the Differently expressed genes (DEGs) of GSE50161 and GSE116520. A Venn diagram revealed 467 common DEGs between the 2 datasets. Lysyl oxidase (LOX), serpin family H member 1 (SERPINH1) and transforming growth factor beta induced (TGFBI) were negatively correlated with the overall survival rate in patients with GBM. The hub genes are high in GBM tumor tissues. The relative expression levels of LOX, SERPINH1 and TGFBI were significantly higher in GBM samples, compared with the normal brain tissues groups. Bioinformatics technology could be a useful tool to predict progression of GBM and to explore the mechanism of GBM.LOX, SERPINH1 and TGFBI may be involved in the mechanism of the occurrence and development of GBM, and may be used as molecular targets for early diagnosis and specific treatment. DEGs identified using GEO2R. Functional annotation of DEGs using Kyoto Encyclopedia of Genes and Genomes and gene body pathway enrichment analysis. Construction of a protein-protein interaction network. The pathway and process enrichment analysis of the hub genes were performed by Metascape. Survival analysis was performed in gene expression profiling interactive analysis. Real-time fluorescent quantitative polymerase chain reaction assay was performed to verify. The animal model was established for western blot test analysis.
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spelling pubmed-96661662022-11-16 Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI Zhang, Shuyuan Zhang, Weiwei Wu, Bin Xia, Liang Li, Liwen Jin, Kai Zou, Yangfan Sun, Caixing Medicine (Baltimore) 5700 Glioblastoma (GBM) is a malignant tumor. The long-term prognosis of the patients is poor. Therefore, it is of important clinical value to further explore the pathogenesis and look for molecular markers for early diagnosis and targeted treatment. Two expression profiling datasets [GSE50161 (GPL570 platform), GSE116520 (GPL10558 platform)] were respectively downloaded from the gene expression omnibus database. Volcano diagrams show the Differently expressed genes (DEGs) of GSE50161 and GSE116520. A Venn diagram revealed 467 common DEGs between the 2 datasets. Lysyl oxidase (LOX), serpin family H member 1 (SERPINH1) and transforming growth factor beta induced (TGFBI) were negatively correlated with the overall survival rate in patients with GBM. The hub genes are high in GBM tumor tissues. The relative expression levels of LOX, SERPINH1 and TGFBI were significantly higher in GBM samples, compared with the normal brain tissues groups. Bioinformatics technology could be a useful tool to predict progression of GBM and to explore the mechanism of GBM.LOX, SERPINH1 and TGFBI may be involved in the mechanism of the occurrence and development of GBM, and may be used as molecular targets for early diagnosis and specific treatment. DEGs identified using GEO2R. Functional annotation of DEGs using Kyoto Encyclopedia of Genes and Genomes and gene body pathway enrichment analysis. Construction of a protein-protein interaction network. The pathway and process enrichment analysis of the hub genes were performed by Metascape. Survival analysis was performed in gene expression profiling interactive analysis. Real-time fluorescent quantitative polymerase chain reaction assay was performed to verify. The animal model was established for western blot test analysis. Lippincott Williams & Wilkins 2022-11-11 /pmc/articles/PMC9666166/ /pubmed/36397358 http://dx.doi.org/10.1097/MD.0000000000031418 Text en Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC) (https://creativecommons.org/licenses/by-nc/4.0/) , where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal.
spellingShingle 5700
Zhang, Shuyuan
Zhang, Weiwei
Wu, Bin
Xia, Liang
Li, Liwen
Jin, Kai
Zou, Yangfan
Sun, Caixing
Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI
title Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI
title_full Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI
title_fullStr Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI
title_full_unstemmed Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI
title_short Hub gene target of glioblastoma: LOX, SERPINH1 and TGFBI
title_sort hub gene target of glioblastoma: lox, serpinh1 and tgfbi
topic 5700
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666166/
https://www.ncbi.nlm.nih.gov/pubmed/36397358
http://dx.doi.org/10.1097/MD.0000000000031418
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