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The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization
Oocyte maturity is critical to the development potential of the embryo and pregnancy outcomes in natural and in-vitro fertilization (IVF). In IVF, oocyte maturity is typically evaluated using morphological criteria, although such assessment remains highly subjective. To identify reliable biomarkers...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666526/ https://www.ncbi.nlm.nih.gov/pubmed/36379980 http://dx.doi.org/10.1038/s41598-022-23737-1 |
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author | Hong, Kun-Jing Lin, Jun-Jie Lin, Liang-Hua Lai, Tsung-Hsuan |
author_facet | Hong, Kun-Jing Lin, Jun-Jie Lin, Liang-Hua Lai, Tsung-Hsuan |
author_sort | Hong, Kun-Jing |
collection | PubMed |
description | Oocyte maturity is critical to the development potential of the embryo and pregnancy outcomes in natural and in-vitro fertilization (IVF). In IVF, oocyte maturity is typically evaluated using morphological criteria, although such assessment remains highly subjective. To identify reliable biomarkers of oocyte maturity, this study investigates the relationship between follicular cytokine concentrations and oocyte maturity in IVF patients with different ovarian reserves. In this prospective study, follicular fluid was collected during oocyte retrieval and the concentrations of cytokines involved in ovarian folliculogenesis were determined. Follicular fluid cytokine concentrations were compared between participants in three groups according to serum anti-Mullerian hormone (AMH) concentration, as follows: low AMH, < 2 ng/mL; normal AMH, 2–5 ng/mL; and high AMH, > 5 ng/mL. Pearson's correlation coefficient analysis showed that the number of mature oocytes correlated positively and strongly with serum AMH level (r = 0.719; p < 0.01). The leptin concentration in follicular fluid was significantly higher in women with normal AMH level than in those with low or high levels. ROC curve analysis showed that the follicular fluid levels of leptin (area under ROC curve, 0.829; 95% confidence interval, 0.659–0.998; p < 0.01) and SCF (area under ROC curve, 0.706; 95% confidence interval, 0.491–0.921; p = 0.087) were the best predictors of oocyte maturity. At an optimal cut-off value of 16 ng/mL, leptin had positive predictive value (sensitivity) up to 70% and negative predictive value (specificity) of 91% for indicating oocyte maturity. The concentration of leptin in follicular fluid is closely related to ovarian reserve and may serve as a biomarker to predict oocyte maturity. |
format | Online Article Text |
id | pubmed-9666526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96665262022-11-17 The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization Hong, Kun-Jing Lin, Jun-Jie Lin, Liang-Hua Lai, Tsung-Hsuan Sci Rep Article Oocyte maturity is critical to the development potential of the embryo and pregnancy outcomes in natural and in-vitro fertilization (IVF). In IVF, oocyte maturity is typically evaluated using morphological criteria, although such assessment remains highly subjective. To identify reliable biomarkers of oocyte maturity, this study investigates the relationship between follicular cytokine concentrations and oocyte maturity in IVF patients with different ovarian reserves. In this prospective study, follicular fluid was collected during oocyte retrieval and the concentrations of cytokines involved in ovarian folliculogenesis were determined. Follicular fluid cytokine concentrations were compared between participants in three groups according to serum anti-Mullerian hormone (AMH) concentration, as follows: low AMH, < 2 ng/mL; normal AMH, 2–5 ng/mL; and high AMH, > 5 ng/mL. Pearson's correlation coefficient analysis showed that the number of mature oocytes correlated positively and strongly with serum AMH level (r = 0.719; p < 0.01). The leptin concentration in follicular fluid was significantly higher in women with normal AMH level than in those with low or high levels. ROC curve analysis showed that the follicular fluid levels of leptin (area under ROC curve, 0.829; 95% confidence interval, 0.659–0.998; p < 0.01) and SCF (area under ROC curve, 0.706; 95% confidence interval, 0.491–0.921; p = 0.087) were the best predictors of oocyte maturity. At an optimal cut-off value of 16 ng/mL, leptin had positive predictive value (sensitivity) up to 70% and negative predictive value (specificity) of 91% for indicating oocyte maturity. The concentration of leptin in follicular fluid is closely related to ovarian reserve and may serve as a biomarker to predict oocyte maturity. Nature Publishing Group UK 2022-11-15 /pmc/articles/PMC9666526/ /pubmed/36379980 http://dx.doi.org/10.1038/s41598-022-23737-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hong, Kun-Jing Lin, Jun-Jie Lin, Liang-Hua Lai, Tsung-Hsuan The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
title | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
title_full | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
title_fullStr | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
title_full_unstemmed | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
title_short | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
title_sort | intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666526/ https://www.ncbi.nlm.nih.gov/pubmed/36379980 http://dx.doi.org/10.1038/s41598-022-23737-1 |
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