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Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?

Productivity decline of Casuarina equisetifolia plantation and difficulty in natural regeneration remains a serious problem because of allelopathy. Previous studies have confirmed that 2,4-di-tert-butylphenol (2,4-DTBP) are the major allelochemicals of the C. equisetifolia litter exudates. The produ...

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Autores principales: Xu, Zhixia, Zuo, Linzhi, Zhang, Yaqian, Huang, Rui, Li, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666782/
https://www.ncbi.nlm.nih.gov/pubmed/36407626
http://dx.doi.org/10.3389/fpls.2022.1022984
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author Xu, Zhixia
Zuo, Linzhi
Zhang, Yaqian
Huang, Rui
Li, Lei
author_facet Xu, Zhixia
Zuo, Linzhi
Zhang, Yaqian
Huang, Rui
Li, Lei
author_sort Xu, Zhixia
collection PubMed
description Productivity decline of Casuarina equisetifolia plantation and difficulty in natural regeneration remains a serious problem because of allelopathy. Previous studies have confirmed that 2,4-di-tert-butylphenol (2,4-DTBP) are the major allelochemicals of the C. equisetifolia litter exudates. The production of these allelochemicals may derive from decomposition of litter or from the litter endophyte and microorganisms adhering to litter surfaces. In the present study, we aimed to evaluate the correlation between allelochemicals in litter and endophytic and epiphytic fungi and bacteria from litter. A total of 100 fungi and 116 bacteria were isolated from the interior and surface of litter of different forest ages (young, half-mature, and mature plantation). Results showed that the fermentation broth of fungal genera Mycosphaerella sp. and Pestalotiopsis sp., and bacterial genera Bacillus amyloliquefaciens, Burkholderia-Paraburkholderia, and Pantoea ananatis had the strongest allelopathic effect on C. equisetifolia seeds. Allelochemicals, such as 2,4-DTBP and its analogs were identified in the fermentation broths of these microorganisms using GC/MS analysis. These results indicate that endophytic and epiphytic fungi and bacteria in litters are involved in the synthesis of allelochemicals of C. equisetifolia. To further determine the abundance of the allelopathic fungi and bacteria, Illumina MiSeq high-throughput sequencing was performed. The results showed that bacterial genera with strong allelopathic potential were mainly distributed in the young and half-mature plantation with low abundance, while the abundance of fungal genera Mycosphaerella sp. and Pestalotiopsis sp. were higher in the young and mature plantations. In particular, the abundance of Mycosphaerella sp. in the young and mature plantations were 501.20% and 192.63% higher than in the half-mature plantation, respectively. Overall, our study demonstrates that the litter fungi with higher abundance in the young and mature plantation were involved in the synthesis of the allelochemical 2,4-DTBP of C. equisetifolia. This finding may be important for understanding the relationship between autotoxicity and microorganism and clarifying the natural regeneration problem of C. equisetifolia.
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spelling pubmed-96667822022-11-17 Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms? Xu, Zhixia Zuo, Linzhi Zhang, Yaqian Huang, Rui Li, Lei Front Plant Sci Plant Science Productivity decline of Casuarina equisetifolia plantation and difficulty in natural regeneration remains a serious problem because of allelopathy. Previous studies have confirmed that 2,4-di-tert-butylphenol (2,4-DTBP) are the major allelochemicals of the C. equisetifolia litter exudates. The production of these allelochemicals may derive from decomposition of litter or from the litter endophyte and microorganisms adhering to litter surfaces. In the present study, we aimed to evaluate the correlation between allelochemicals in litter and endophytic and epiphytic fungi and bacteria from litter. A total of 100 fungi and 116 bacteria were isolated from the interior and surface of litter of different forest ages (young, half-mature, and mature plantation). Results showed that the fermentation broth of fungal genera Mycosphaerella sp. and Pestalotiopsis sp., and bacterial genera Bacillus amyloliquefaciens, Burkholderia-Paraburkholderia, and Pantoea ananatis had the strongest allelopathic effect on C. equisetifolia seeds. Allelochemicals, such as 2,4-DTBP and its analogs were identified in the fermentation broths of these microorganisms using GC/MS analysis. These results indicate that endophytic and epiphytic fungi and bacteria in litters are involved in the synthesis of allelochemicals of C. equisetifolia. To further determine the abundance of the allelopathic fungi and bacteria, Illumina MiSeq high-throughput sequencing was performed. The results showed that bacterial genera with strong allelopathic potential were mainly distributed in the young and half-mature plantation with low abundance, while the abundance of fungal genera Mycosphaerella sp. and Pestalotiopsis sp. were higher in the young and mature plantations. In particular, the abundance of Mycosphaerella sp. in the young and mature plantations were 501.20% and 192.63% higher than in the half-mature plantation, respectively. Overall, our study demonstrates that the litter fungi with higher abundance in the young and mature plantation were involved in the synthesis of the allelochemical 2,4-DTBP of C. equisetifolia. This finding may be important for understanding the relationship between autotoxicity and microorganism and clarifying the natural regeneration problem of C. equisetifolia. Frontiers Media S.A. 2022-11-02 /pmc/articles/PMC9666782/ /pubmed/36407626 http://dx.doi.org/10.3389/fpls.2022.1022984 Text en Copyright © 2022 Xu, Zuo, Zhang, Huang and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Zhixia
Zuo, Linzhi
Zhang, Yaqian
Huang, Rui
Li, Lei
Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?
title Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?
title_full Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?
title_fullStr Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?
title_full_unstemmed Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?
title_short Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?
title_sort is allelochemical synthesis in casuarina equisetifolia plantation related to litter microorganisms?
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9666782/
https://www.ncbi.nlm.nih.gov/pubmed/36407626
http://dx.doi.org/10.3389/fpls.2022.1022984
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