A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection

Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe i...

Descripción completa

Detalles Bibliográficos
Autores principales: Gong, Xiu-Ying, Zhang, Yi-Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667292/
https://www.ncbi.nlm.nih.gov/pubmed/36595883
http://dx.doi.org/10.1016/j.xpro.2022.101844
Descripción
Sumario:Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe in vitro assays including titration of infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus infection. For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).(1)

Ejemplares similares