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New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal

Visceral leishmaniasis is not endemic in West Africa, but prevalence of canine leishmaniasis and seroprevalence of Leishmania infantum infection in humans are high in the Mont Rolland community (Thiès region, Senegal). Previous studies in this area showed that Sergentomyia schwetzi could be the pote...

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Autores principales: Prudhomme, Jorian, Mazza, Thomas, Hagen, Sophie, Cassan, Cécile, Toty, Céline, Senghor, Massila Wagué, Niang, Abdoul Aziz, Faye, Babacar, Bañuls, Anne-Laure
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667725/
https://www.ncbi.nlm.nih.gov/pubmed/35980597
http://dx.doi.org/10.1093/jme/tjac109
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author Prudhomme, Jorian
Mazza, Thomas
Hagen, Sophie
Cassan, Cécile
Toty, Céline
Senghor, Massila Wagué
Niang, Abdoul Aziz
Faye, Babacar
Bañuls, Anne-Laure
author_facet Prudhomme, Jorian
Mazza, Thomas
Hagen, Sophie
Cassan, Cécile
Toty, Céline
Senghor, Massila Wagué
Niang, Abdoul Aziz
Faye, Babacar
Bañuls, Anne-Laure
author_sort Prudhomme, Jorian
collection PubMed
description Visceral leishmaniasis is not endemic in West Africa, but prevalence of canine leishmaniasis and seroprevalence of Leishmania infantum infection in humans are high in the Mont Rolland community (Thiès region, Senegal). Previous studies in this area showed that Sergentomyia schwetzi could be the potential vector of Le. infantum. To precisely describe the biology and population structure of this potential vector, we identified eight novel microsatellite loci to characterize Se. schwetzi populations. We tested these loci in Se. schwetzi populations from five locations at Mont Rolland (Thiès, Senegal). All the loci were polymorphic, with a mean of 17.25 alleles (observed heterozygosity: 0.455). We did not detect any evidence of scoring errors due to stuttering and large allele dropout. Moreover, several of these loci were also amplified in six other sand fly species (Sergentomyia magna, Sergentomyia dubia, Sergentomyia minuta, Phlebotomus duboscqi, Phlebotomus perniciosus, and Phlebotomus ariasi). These preliminary results demonstrate the utility of these microsatellite markers for Se. schwetzi (and for the other sand fly species) population genetic studies.
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spelling pubmed-96677252022-11-17 New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal Prudhomme, Jorian Mazza, Thomas Hagen, Sophie Cassan, Cécile Toty, Céline Senghor, Massila Wagué Niang, Abdoul Aziz Faye, Babacar Bañuls, Anne-Laure J Med Entomol Short Communications Visceral leishmaniasis is not endemic in West Africa, but prevalence of canine leishmaniasis and seroprevalence of Leishmania infantum infection in humans are high in the Mont Rolland community (Thiès region, Senegal). Previous studies in this area showed that Sergentomyia schwetzi could be the potential vector of Le. infantum. To precisely describe the biology and population structure of this potential vector, we identified eight novel microsatellite loci to characterize Se. schwetzi populations. We tested these loci in Se. schwetzi populations from five locations at Mont Rolland (Thiès, Senegal). All the loci were polymorphic, with a mean of 17.25 alleles (observed heterozygosity: 0.455). We did not detect any evidence of scoring errors due to stuttering and large allele dropout. Moreover, several of these loci were also amplified in six other sand fly species (Sergentomyia magna, Sergentomyia dubia, Sergentomyia minuta, Phlebotomus duboscqi, Phlebotomus perniciosus, and Phlebotomus ariasi). These preliminary results demonstrate the utility of these microsatellite markers for Se. schwetzi (and for the other sand fly species) population genetic studies. Oxford University Press 2022-08-18 /pmc/articles/PMC9667725/ /pubmed/35980597 http://dx.doi.org/10.1093/jme/tjac109 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Short Communications
Prudhomme, Jorian
Mazza, Thomas
Hagen, Sophie
Cassan, Cécile
Toty, Céline
Senghor, Massila Wagué
Niang, Abdoul Aziz
Faye, Babacar
Bañuls, Anne-Laure
New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal
title New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal
title_full New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal
title_fullStr New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal
title_full_unstemmed New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal
title_short New Microsatellite Markers for Genetic Studies on Sergentomyia schwetzi (Diptera: Psychodidae): A Suspected Vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal
title_sort new microsatellite markers for genetic studies on sergentomyia schwetzi (diptera: psychodidae): a suspected vector of leishmania infantum (trypanosomatida: trypanosomatidae) in the canine leishmaniasis focus of mont-rolland, senegal
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667725/
https://www.ncbi.nlm.nih.gov/pubmed/35980597
http://dx.doi.org/10.1093/jme/tjac109
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