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Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer

Circular RNA (circRNA), a novel class of non-coding RNA, has been reported in various diseases, especially in tumors. However, the key signatures of circRNA-competitive endogenous RNA (ceRNA) network are largely unclear in colorectal cancer (CRC). We first characterized circRNAs profile by using cir...

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Autores principales: Gao, Yan, Zhou, Yulai, Wei, Le, Feng, Ziyang, Chen, Yihong, Liu, Ping, Peng, Yinghui, Huang, Qiaoqiao, Gao, Le, Liu, Yongting, Han, Ying, Shen, Hong, Cai, Changjing, Zeng, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667793/
https://www.ncbi.nlm.nih.gov/pubmed/36405685
http://dx.doi.org/10.3389/fimmu.2022.927811
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author Gao, Yan
Zhou, Yulai
Wei, Le
Feng, Ziyang
Chen, Yihong
Liu, Ping
Peng, Yinghui
Huang, Qiaoqiao
Gao, Le
Liu, Yongting
Han, Ying
Shen, Hong
Cai, Changjing
Zeng, Shan
author_facet Gao, Yan
Zhou, Yulai
Wei, Le
Feng, Ziyang
Chen, Yihong
Liu, Ping
Peng, Yinghui
Huang, Qiaoqiao
Gao, Le
Liu, Yongting
Han, Ying
Shen, Hong
Cai, Changjing
Zeng, Shan
author_sort Gao, Yan
collection PubMed
description Circular RNA (circRNA), a novel class of non-coding RNA, has been reported in various diseases, especially in tumors. However, the key signatures of circRNA-competitive endogenous RNA (ceRNA) network are largely unclear in colorectal cancer (CRC). We first characterized circRNAs profile by using circRNA-seq analysis from real-word dataset. The expression level of hsa_circ_0066351 in CRC tissues and cell lines was detected by quantitative real-time PCR. Then, cell proliferation assay was used to confirm the proliferation function of hsa_circ_0066351. Next, Cytoscape was used to construct circRNA–miRNA–mRNA networks. Last but not least, the landscape of hsa_circ_0066351–miRNA–mRNA in CRC had been investigated in the bulk tissue RNA-Seq level and single-cell Seq level. We proved that hsa_circ_0066351 was significantly downregulated in CRC cell lines and tissues (P < 0.001), and was negatively associated with distant metastasis (P < 0.01). Significantly, the expression of hsa_circ_0066351 was associated with better survival in patients with CRC. Function assays showed that hsa_circ_0066351 could inhibit CRC cells proliferation. In addition, a ceRNA network, including hsa_circ_0066351, two miRNAs, and ten mRNAs, was constructed. Our analyses showed that these ten mRNAs were consistently downregulated in pan-cancer and enriched in tumor suppressive function. A risk score model constructed by these ten downstream genes also indicated that they were related to the prognosis and immune response in CRC. In conclusion, we demonstrated that a novel circRNA (hsa_circ_0066351) inhibited CRC proliferation, and revealed a potential prognostic and immunotherapeutic biomarker in CRC.
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spelling pubmed-96677932022-11-17 Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer Gao, Yan Zhou, Yulai Wei, Le Feng, Ziyang Chen, Yihong Liu, Ping Peng, Yinghui Huang, Qiaoqiao Gao, Le Liu, Yongting Han, Ying Shen, Hong Cai, Changjing Zeng, Shan Front Immunol Immunology Circular RNA (circRNA), a novel class of non-coding RNA, has been reported in various diseases, especially in tumors. However, the key signatures of circRNA-competitive endogenous RNA (ceRNA) network are largely unclear in colorectal cancer (CRC). We first characterized circRNAs profile by using circRNA-seq analysis from real-word dataset. The expression level of hsa_circ_0066351 in CRC tissues and cell lines was detected by quantitative real-time PCR. Then, cell proliferation assay was used to confirm the proliferation function of hsa_circ_0066351. Next, Cytoscape was used to construct circRNA–miRNA–mRNA networks. Last but not least, the landscape of hsa_circ_0066351–miRNA–mRNA in CRC had been investigated in the bulk tissue RNA-Seq level and single-cell Seq level. We proved that hsa_circ_0066351 was significantly downregulated in CRC cell lines and tissues (P < 0.001), and was negatively associated with distant metastasis (P < 0.01). Significantly, the expression of hsa_circ_0066351 was associated with better survival in patients with CRC. Function assays showed that hsa_circ_0066351 could inhibit CRC cells proliferation. In addition, a ceRNA network, including hsa_circ_0066351, two miRNAs, and ten mRNAs, was constructed. Our analyses showed that these ten mRNAs were consistently downregulated in pan-cancer and enriched in tumor suppressive function. A risk score model constructed by these ten downstream genes also indicated that they were related to the prognosis and immune response in CRC. In conclusion, we demonstrated that a novel circRNA (hsa_circ_0066351) inhibited CRC proliferation, and revealed a potential prognostic and immunotherapeutic biomarker in CRC. Frontiers Media S.A. 2022-07-13 /pmc/articles/PMC9667793/ /pubmed/36405685 http://dx.doi.org/10.3389/fimmu.2022.927811 Text en Copyright © 2022 Gao, Zhou, Wei, Feng, Chen, Liu, Peng, Huang, Gao, Liu, Han, Shen, Cai and Zeng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Gao, Yan
Zhou, Yulai
Wei, Le
Feng, Ziyang
Chen, Yihong
Liu, Ping
Peng, Yinghui
Huang, Qiaoqiao
Gao, Le
Liu, Yongting
Han, Ying
Shen, Hong
Cai, Changjing
Zeng, Shan
Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer
title Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer
title_full Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer
title_fullStr Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer
title_full_unstemmed Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer
title_short Hsa_Circ_0066351 Acts as a Prognostic and Immunotherapeutic Biomarker in Colorectal Cancer
title_sort hsa_circ_0066351 acts as a prognostic and immunotherapeutic biomarker in colorectal cancer
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667793/
https://www.ncbi.nlm.nih.gov/pubmed/36405685
http://dx.doi.org/10.3389/fimmu.2022.927811
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