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A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”

Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African cou...

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Autores principales: Marion, Estelle, Hycenth, Numfor, Vedithi, Sundeep Chaitanya, Robbe-Saule, Marie, Donkeng, Valérie, Ganlonon, Line-Marlène, Dissou, Affolabi, Ngazoa, Solange Kakou, Kabedi, Marie-Jose, Mabika Mabika, Arsène, Phillips, Richard, Frimpong, Michael, Yeboah-Manu, Dorothy, Walker, Vera Yatta, Akinwale, Olaoluwa, Issaka, Maman, Bretzel, Gisela, Asiedu, Kingsley, Eyangoh, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668193/
https://www.ncbi.nlm.nih.gov/pubmed/36331971
http://dx.doi.org/10.1371/journal.pntd.0010908
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author Marion, Estelle
Hycenth, Numfor
Vedithi, Sundeep Chaitanya
Robbe-Saule, Marie
Donkeng, Valérie
Ganlonon, Line-Marlène
Dissou, Affolabi
Ngazoa, Solange Kakou
Kabedi, Marie-Jose
Mabika Mabika, Arsène
Phillips, Richard
Frimpong, Michael
Yeboah-Manu, Dorothy
Walker, Vera Yatta
Akinwale, Olaoluwa
Issaka, Maman
Bretzel, Gisela
Asiedu, Kingsley
Eyangoh, Sara
author_facet Marion, Estelle
Hycenth, Numfor
Vedithi, Sundeep Chaitanya
Robbe-Saule, Marie
Donkeng, Valérie
Ganlonon, Line-Marlène
Dissou, Affolabi
Ngazoa, Solange Kakou
Kabedi, Marie-Jose
Mabika Mabika, Arsène
Phillips, Richard
Frimpong, Michael
Yeboah-Manu, Dorothy
Walker, Vera Yatta
Akinwale, Olaoluwa
Issaka, Maman
Bretzel, Gisela
Asiedu, Kingsley
Eyangoh, Sara
author_sort Marion, Estelle
collection PubMed
description Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African countries, South America and Western Pacific regions. Majority of cases are spread across West and Central Africa. The mode of transmission is unclear, hindering the implementation of adequate prevention for the population. Currently, early diagnosis and treatment are crucial to minimizing morbidity, costs and preventing long-term disability. Biological confirmation of clinical diagnosis of Buruli ulcer is essential before starting chemotherapy. Indeed, differential diagnosis are numerous and Buruli ulcer has varying clinical presentations. Up to now, the gold standard biological confirmation is the quantitative PCR, targeting the insertion sequence IS2404 of M. ulcerans performed on cutaneous samples. Due to the low PCR confirmation rate in endemic African countries (under 30% in 2018) for numerous identified reasons within this article, 11 laboratories decided to combine their efforts to create the network “BU-LABNET” in 2019. The first step of the network was to harmonize the procedures and ship specific reagents to each laboratory. With this system in place, implementation of these procedures for testing and follow-up was easy and the laboratories were able to carry out their first quality control with a very high success rate. It is now time to integrate other neglected tropical diseases to this platform, such as yaws or leprosy.
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spelling pubmed-96681932022-11-17 A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET” Marion, Estelle Hycenth, Numfor Vedithi, Sundeep Chaitanya Robbe-Saule, Marie Donkeng, Valérie Ganlonon, Line-Marlène Dissou, Affolabi Ngazoa, Solange Kakou Kabedi, Marie-Jose Mabika Mabika, Arsène Phillips, Richard Frimpong, Michael Yeboah-Manu, Dorothy Walker, Vera Yatta Akinwale, Olaoluwa Issaka, Maman Bretzel, Gisela Asiedu, Kingsley Eyangoh, Sara PLoS Negl Trop Dis Research Article Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African countries, South America and Western Pacific regions. Majority of cases are spread across West and Central Africa. The mode of transmission is unclear, hindering the implementation of adequate prevention for the population. Currently, early diagnosis and treatment are crucial to minimizing morbidity, costs and preventing long-term disability. Biological confirmation of clinical diagnosis of Buruli ulcer is essential before starting chemotherapy. Indeed, differential diagnosis are numerous and Buruli ulcer has varying clinical presentations. Up to now, the gold standard biological confirmation is the quantitative PCR, targeting the insertion sequence IS2404 of M. ulcerans performed on cutaneous samples. Due to the low PCR confirmation rate in endemic African countries (under 30% in 2018) for numerous identified reasons within this article, 11 laboratories decided to combine their efforts to create the network “BU-LABNET” in 2019. The first step of the network was to harmonize the procedures and ship specific reagents to each laboratory. With this system in place, implementation of these procedures for testing and follow-up was easy and the laboratories were able to carry out their first quality control with a very high success rate. It is now time to integrate other neglected tropical diseases to this platform, such as yaws or leprosy. Public Library of Science 2022-11-04 /pmc/articles/PMC9668193/ /pubmed/36331971 http://dx.doi.org/10.1371/journal.pntd.0010908 Text en © 2022 Marion et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Marion, Estelle
Hycenth, Numfor
Vedithi, Sundeep Chaitanya
Robbe-Saule, Marie
Donkeng, Valérie
Ganlonon, Line-Marlène
Dissou, Affolabi
Ngazoa, Solange Kakou
Kabedi, Marie-Jose
Mabika Mabika, Arsène
Phillips, Richard
Frimpong, Michael
Yeboah-Manu, Dorothy
Walker, Vera Yatta
Akinwale, Olaoluwa
Issaka, Maman
Bretzel, Gisela
Asiedu, Kingsley
Eyangoh, Sara
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
title A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
title_full A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
title_fullStr A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
title_full_unstemmed A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
title_short A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
title_sort combined effort of 11 laboratories in the who african region to improve quality of buruli ulcer pcr diagnosis: the “bu-labnet”
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668193/
https://www.ncbi.nlm.nih.gov/pubmed/36331971
http://dx.doi.org/10.1371/journal.pntd.0010908
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