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Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction

RATIONALE: Opioid use disorders are commonly treated by long-acting agonist opioids including methadone and buprenorphine which could affect various aspects of male reproduction especially spermatogenesis. OBJECTIVES: We aimed to determine whether detoxification with methadone or buprenorphine was a...

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Autores principales: Moinaddini, Fatemeh, Amirinejad, Maryam, Haghpanah, Tahereh, Abedini, Mohsen, Yoosefi, Farhad, Nematollahi-mahani, Seyed noureddin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668397/
https://www.ncbi.nlm.nih.gov/pubmed/36385208
http://dx.doi.org/10.1007/s00213-022-06274-7
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author Moinaddini, Fatemeh
Amirinejad, Maryam
Haghpanah, Tahereh
Abedini, Mohsen
Yoosefi, Farhad
Nematollahi-mahani, Seyed noureddin
author_facet Moinaddini, Fatemeh
Amirinejad, Maryam
Haghpanah, Tahereh
Abedini, Mohsen
Yoosefi, Farhad
Nematollahi-mahani, Seyed noureddin
author_sort Moinaddini, Fatemeh
collection PubMed
description RATIONALE: Opioid use disorders are commonly treated by long-acting agonist opioids including methadone and buprenorphine which could affect various aspects of male reproduction especially spermatogenesis. OBJECTIVES: We aimed to determine whether detoxification with methadone or buprenorphine was associated with reproductive disorders in male mice. METHODS: We orally induced morphine dependence in NMRI male mice, and then performed detoxification programs using either methadone or buprenorphine. Testis architecture and sperm parameters including sperm nuclear DNA integrity, mitochondrial activity, oxidative stress in seminal plasma, and routine sperm parameters were assessed to find the involved mechanisms. RESULTS: The number of Leydig cells and the thickness of germinal epithelium reduced following morphine use and increased differently after detoxification with methadone or buprenorphine. Morphine dependence and detoxification with methadone and buprenorphine had different effects on sperm parameters. Morphine altered chromatin integrity, mitochondrial activity, and oxidative stress in sperm. Detoxification with methadone improved mitochondrial activity but worsened chromatin integrity, whereas detoxification with buprenorphine improved neither chromatin integrity nor mitochondrial activity. Seminal plasma oxidative stress was higher in the treated groups compared to control groups but was comparable among treatment groups. Our study revealed that long-term morphine use followed by detoxification with methadone or buprenorphine impairs testis structure and sperm parameters. Detoxification from morphine use with methadone and buprenorphine led to different preclinical outcomes in semen quality parameters, including chromatin integrity. Therefore, clinical detoxification protocols should be performed more cautiously, considering the desire of the individuals to reproduce.
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spelling pubmed-96683972022-11-18 Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction Moinaddini, Fatemeh Amirinejad, Maryam Haghpanah, Tahereh Abedini, Mohsen Yoosefi, Farhad Nematollahi-mahani, Seyed noureddin Psychopharmacology (Berl) Original Investigation RATIONALE: Opioid use disorders are commonly treated by long-acting agonist opioids including methadone and buprenorphine which could affect various aspects of male reproduction especially spermatogenesis. OBJECTIVES: We aimed to determine whether detoxification with methadone or buprenorphine was associated with reproductive disorders in male mice. METHODS: We orally induced morphine dependence in NMRI male mice, and then performed detoxification programs using either methadone or buprenorphine. Testis architecture and sperm parameters including sperm nuclear DNA integrity, mitochondrial activity, oxidative stress in seminal plasma, and routine sperm parameters were assessed to find the involved mechanisms. RESULTS: The number of Leydig cells and the thickness of germinal epithelium reduced following morphine use and increased differently after detoxification with methadone or buprenorphine. Morphine dependence and detoxification with methadone and buprenorphine had different effects on sperm parameters. Morphine altered chromatin integrity, mitochondrial activity, and oxidative stress in sperm. Detoxification with methadone improved mitochondrial activity but worsened chromatin integrity, whereas detoxification with buprenorphine improved neither chromatin integrity nor mitochondrial activity. Seminal plasma oxidative stress was higher in the treated groups compared to control groups but was comparable among treatment groups. Our study revealed that long-term morphine use followed by detoxification with methadone or buprenorphine impairs testis structure and sperm parameters. Detoxification from morphine use with methadone and buprenorphine led to different preclinical outcomes in semen quality parameters, including chromatin integrity. Therefore, clinical detoxification protocols should be performed more cautiously, considering the desire of the individuals to reproduce. Springer Berlin Heidelberg 2022-11-17 2023 /pmc/articles/PMC9668397/ /pubmed/36385208 http://dx.doi.org/10.1007/s00213-022-06274-7 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Investigation
Moinaddini, Fatemeh
Amirinejad, Maryam
Haghpanah, Tahereh
Abedini, Mohsen
Yoosefi, Farhad
Nematollahi-mahani, Seyed noureddin
Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
title Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
title_full Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
title_fullStr Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
title_full_unstemmed Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
title_short Opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
title_sort opioid replacement therapy with methadone or buprenorphine effects on male mice reproduction
topic Original Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668397/
https://www.ncbi.nlm.nih.gov/pubmed/36385208
http://dx.doi.org/10.1007/s00213-022-06274-7
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