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Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso

The emergence of antimicrobial-resistantfood-borne bacteria is a great challenge to public health. This study was conducted to characterize and determine the resistance profile of Salmonella strains isolated from foods including sesames, ready-to-eat (RTE) salads, mango juices, and lettuce in Burkin...

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Autores principales: Soubeiga, Adama Patrice, Kpoda, Dissinviel Stéphane, Compaoré, Muller K. A., Somda-Belemlougri, Asseto, Kaseko, Ndamiwe, Rouamba, Sibiri Sylvain, Ouedraogo, Sandrine, Traoré, Roukiatou, Karfo, Paulette, Nezien, Désiré, Nikiéma, Fulbert, Kabre, Elie, Zongo, Cheikna, Savadogo, Aly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668442/
https://www.ncbi.nlm.nih.gov/pubmed/36406904
http://dx.doi.org/10.1155/2022/9640828
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author Soubeiga, Adama Patrice
Kpoda, Dissinviel Stéphane
Compaoré, Muller K. A.
Somda-Belemlougri, Asseto
Kaseko, Ndamiwe
Rouamba, Sibiri Sylvain
Ouedraogo, Sandrine
Traoré, Roukiatou
Karfo, Paulette
Nezien, Désiré
Nikiéma, Fulbert
Kabre, Elie
Zongo, Cheikna
Savadogo, Aly
author_facet Soubeiga, Adama Patrice
Kpoda, Dissinviel Stéphane
Compaoré, Muller K. A.
Somda-Belemlougri, Asseto
Kaseko, Ndamiwe
Rouamba, Sibiri Sylvain
Ouedraogo, Sandrine
Traoré, Roukiatou
Karfo, Paulette
Nezien, Désiré
Nikiéma, Fulbert
Kabre, Elie
Zongo, Cheikna
Savadogo, Aly
author_sort Soubeiga, Adama Patrice
collection PubMed
description The emergence of antimicrobial-resistantfood-borne bacteria is a great challenge to public health. This study was conducted to characterize and determine the resistance profile of Salmonella strains isolated from foods including sesames, ready-to-eat (RTE) salads, mango juices, and lettuce in Burkina Faso. One hundred and forty-eight biochemically identified Salmonella isolates were characterized by molecular amplification of Salmonella marker invA and spiC, misL, orfL, and pipD virulence genes. After that, all confirmed strains were examined for susceptibility to sixteen antimicrobials, and PCR amplifications were used to identify the following resistance genes: bla(TEM), temA, temB, StrA, aadA, sul1, sul2, tet(A), and tet(B). One hundred and eight isolates were genetically confirmed as Salmonella spp. Virulence genes were observed in 57.4%, 55.6%, 49.1%, and 38% isolates for pipD, SpiC, misL, and orfL, respectively. Isolates have shown moderate resistance to gentamycin (26.8%), ampicillin (22.2%), cefoxitin (19.4%), and nalidixic acid (18.5%). All isolates were sensitive to six antibiotics, including cefotaxime, ceftazidime, aztreonam, imipenem, meropenem, and ciprofloxacin. Among the 66 isolates resistant to at least one antibiotic, 11 (16.7%) were multidrug resistant. The Multiple Antimicrobial Resistance (MAR) index of Salmonella serovars ranged from 0.06 to 0.53. PCR detected 7 resistance genes (tet(A), tet(B), bla(TEM), temB, sul1, sul2, and aadA) in drug-resistant isolates. These findings raise serious concerns because ready-to-eat food in Burkina Faso could serve as a reservoir for spreading antimicrobial resistance genes worldwide.
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spelling pubmed-96684422022-11-17 Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso Soubeiga, Adama Patrice Kpoda, Dissinviel Stéphane Compaoré, Muller K. A. Somda-Belemlougri, Asseto Kaseko, Ndamiwe Rouamba, Sibiri Sylvain Ouedraogo, Sandrine Traoré, Roukiatou Karfo, Paulette Nezien, Désiré Nikiéma, Fulbert Kabre, Elie Zongo, Cheikna Savadogo, Aly Int J Microbiol Research Article The emergence of antimicrobial-resistantfood-borne bacteria is a great challenge to public health. This study was conducted to characterize and determine the resistance profile of Salmonella strains isolated from foods including sesames, ready-to-eat (RTE) salads, mango juices, and lettuce in Burkina Faso. One hundred and forty-eight biochemically identified Salmonella isolates were characterized by molecular amplification of Salmonella marker invA and spiC, misL, orfL, and pipD virulence genes. After that, all confirmed strains were examined for susceptibility to sixteen antimicrobials, and PCR amplifications were used to identify the following resistance genes: bla(TEM), temA, temB, StrA, aadA, sul1, sul2, tet(A), and tet(B). One hundred and eight isolates were genetically confirmed as Salmonella spp. Virulence genes were observed in 57.4%, 55.6%, 49.1%, and 38% isolates for pipD, SpiC, misL, and orfL, respectively. Isolates have shown moderate resistance to gentamycin (26.8%), ampicillin (22.2%), cefoxitin (19.4%), and nalidixic acid (18.5%). All isolates were sensitive to six antibiotics, including cefotaxime, ceftazidime, aztreonam, imipenem, meropenem, and ciprofloxacin. Among the 66 isolates resistant to at least one antibiotic, 11 (16.7%) were multidrug resistant. The Multiple Antimicrobial Resistance (MAR) index of Salmonella serovars ranged from 0.06 to 0.53. PCR detected 7 resistance genes (tet(A), tet(B), bla(TEM), temB, sul1, sul2, and aadA) in drug-resistant isolates. These findings raise serious concerns because ready-to-eat food in Burkina Faso could serve as a reservoir for spreading antimicrobial resistance genes worldwide. Hindawi 2022-11-09 /pmc/articles/PMC9668442/ /pubmed/36406904 http://dx.doi.org/10.1155/2022/9640828 Text en Copyright © 2022 Adama Patrice Soubeiga et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Soubeiga, Adama Patrice
Kpoda, Dissinviel Stéphane
Compaoré, Muller K. A.
Somda-Belemlougri, Asseto
Kaseko, Ndamiwe
Rouamba, Sibiri Sylvain
Ouedraogo, Sandrine
Traoré, Roukiatou
Karfo, Paulette
Nezien, Désiré
Nikiéma, Fulbert
Kabre, Elie
Zongo, Cheikna
Savadogo, Aly
Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso
title Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso
title_full Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso
title_fullStr Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso
title_full_unstemmed Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso
title_short Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso
title_sort molecular characterization and the antimicrobial resistance profile of salmonella spp. isolated from ready-to-eat foods in ouagadougou, burkina faso
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668442/
https://www.ncbi.nlm.nih.gov/pubmed/36406904
http://dx.doi.org/10.1155/2022/9640828
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