Cargando…
Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis
Centimeter-scale tissue with angiogenesis has become more and more significant in organ regeneration and drug screening. However, traditional bioink has obvious limitations such as balance of nutrient supporting, printability, and vascularization. Here, with “secondary bioprinting” of printed micros...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Whioce Publishing Pte. Ltd.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668484/ https://www.ncbi.nlm.nih.gov/pubmed/36404788 http://dx.doi.org/10.18063/ijb.v8i4.599 |
_version_ | 1784831923539935232 |
---|---|
author | Xie, Mingjun Sun, Yuan Wang, Ji Fu, Zhenliang Pan, Lei Chen, Zichen Fu, Jianzhong He, Yong |
author_facet | Xie, Mingjun Sun, Yuan Wang, Ji Fu, Zhenliang Pan, Lei Chen, Zichen Fu, Jianzhong He, Yong |
author_sort | Xie, Mingjun |
collection | PubMed |
description | Centimeter-scale tissue with angiogenesis has become more and more significant in organ regeneration and drug screening. However, traditional bioink has obvious limitations such as balance of nutrient supporting, printability, and vascularization. Here, with “secondary bioprinting” of printed microspheres, an innovative bioink system was proposed, in which the thermo-crosslinked sacrificial gelatin microspheres encapsulating human umbilical vein endothelial cells (HUVECs) printed by electrospraying serve as auxiliary component while gelatin methacryloyl precursor solution mixed with subject cells serve as subject component. Benefiting from the reversible thermo-crosslinking feature, gelatin microspheres would experience solid-liquid conversion during 37°C culturing and form controllable porous nutrient network for promoting the nutrient/oxygen delivery in large-scale tissue and accelerate the functionalization of the encapsulated cells. Meanwhile, the encapsulated HUVECs would be released and attach to the pore boundary, which would further form three-dimensional vessel network inside the tissue with suitable inducing conditions. As an example, vascularized breast tumor tissue over 1 cm was successfully built and the HUVECs showed obvious sprout inside, which indicate the great potential of this bioink system in various biomedical applications. |
format | Online Article Text |
id | pubmed-9668484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Whioce Publishing Pte. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96684842022-11-17 Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis Xie, Mingjun Sun, Yuan Wang, Ji Fu, Zhenliang Pan, Lei Chen, Zichen Fu, Jianzhong He, Yong Int J Bioprint Research Article Centimeter-scale tissue with angiogenesis has become more and more significant in organ regeneration and drug screening. However, traditional bioink has obvious limitations such as balance of nutrient supporting, printability, and vascularization. Here, with “secondary bioprinting” of printed microspheres, an innovative bioink system was proposed, in which the thermo-crosslinked sacrificial gelatin microspheres encapsulating human umbilical vein endothelial cells (HUVECs) printed by electrospraying serve as auxiliary component while gelatin methacryloyl precursor solution mixed with subject cells serve as subject component. Benefiting from the reversible thermo-crosslinking feature, gelatin microspheres would experience solid-liquid conversion during 37°C culturing and form controllable porous nutrient network for promoting the nutrient/oxygen delivery in large-scale tissue and accelerate the functionalization of the encapsulated cells. Meanwhile, the encapsulated HUVECs would be released and attach to the pore boundary, which would further form three-dimensional vessel network inside the tissue with suitable inducing conditions. As an example, vascularized breast tumor tissue over 1 cm was successfully built and the HUVECs showed obvious sprout inside, which indicate the great potential of this bioink system in various biomedical applications. Whioce Publishing Pte. Ltd. 2022-08-04 /pmc/articles/PMC9668484/ /pubmed/36404788 http://dx.doi.org/10.18063/ijb.v8i4.599 Text en Copyright: © 2022 Xie et al. https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License, permitting all noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xie, Mingjun Sun, Yuan Wang, Ji Fu, Zhenliang Pan, Lei Chen, Zichen Fu, Jianzhong He, Yong Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis |
title | Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis |
title_full | Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis |
title_fullStr | Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis |
title_full_unstemmed | Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis |
title_short | Thermo-sensitive Sacrificial Microsphere-based Bioink for Centimeter-scale Tissue with Angiogenesis |
title_sort | thermo-sensitive sacrificial microsphere-based bioink for centimeter-scale tissue with angiogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668484/ https://www.ncbi.nlm.nih.gov/pubmed/36404788 http://dx.doi.org/10.18063/ijb.v8i4.599 |
work_keys_str_mv | AT xiemingjun thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT sunyuan thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT wangji thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT fuzhenliang thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT panlei thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT chenzichen thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT fujianzhong thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis AT heyong thermosensitivesacrificialmicrospherebasedbioinkforcentimeterscaletissuewithangiogenesis |