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Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for in...

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Detalles Bibliográficos
Autores principales: Benmeziane, Keltouma, Delache, Benoit, Langlois, Sébastien, Scarlatti, Gabriella, Le Grand, Roger, Cavarelli, Mariangela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668604/
https://www.ncbi.nlm.nih.gov/pubmed/36595919
http://dx.doi.org/10.1016/j.xpro.2022.101815
Descripción
Sumario:Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications. For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022).