Cargando…

Processing and cryopreservation of human ureter tissues for single-cell and spatial transcriptomics assays

Characterizing the cellular heterogeneity of human ureter tissues using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics provides a detailed atlas of cell types, signaling networks, and potential cell-cell cross talk underlying developmental and regenerative pathways. We describe a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fink, Emily E., Sona, Surbhi, Lee, Byron H., Ting, Angela H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668730/ https://www.ncbi.nlm.nih.gov/pubmed/36595885 http://dx.doi.org/10.1016/j.xpro.2022.101854

Ejemplares similares

Protocols for single-cell RNA-seq and spatial gene expression integration and interactive visualization
por: Sona, Surbhi, et al.
Publicado: (2023)

Single-cell transcriptome conservation in cryopreserved cells and tissues
por: Guillaumet-Adkins, Amy, et al.
Publicado: (2017)

Laser Microdissection-Mediated Isolation of Butterfly Wing Tissue for Spatial Transcriptomics
por: Banerjee, Tirtha Das, et al.
Publicado: (2022)

Processing human skin samples for single-cell assays
por: Saluzzo, Simona, et al.
Publicado: (2022)

Processing human lymph node samples for single-cell assays
por: Roider, Tobias, et al.
Publicado: (2021)

Protocol for using MULTILAYER to reveal molecular tissue substructures from digitized spatial transcriptomes
por: Moehlin, Julien, et al.
Publicado: (2021)

Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections
por: Honda, Mizuki, et al.
Publicado: (2022)

Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues
por: Martinek, Jan, et al.
Publicado: (2022)

Protocol for establishing primary human lung organoid-derived air-liquid interface cultures from cryopreserved human lung tissue
por: Castaneda, Diana Cadena, et al.
Publicado: (2023)

Equine Oviductal Organoid Generation and Cryopreservation
por: Thompson, Riley E., et al.
Publicado: (2022)

Localization of T cell clonotypes using the Visium spatial transcriptomics platform
por: Hudson, William H., et al.
Publicado: (2022)

Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
por: Sei, Yoshitaka J., et al.
Publicado: (2023)

Processing Human Thymic Tissue for Single Cell RNA-Seq
por: Le, Justin, et al.
Publicado: (2020)

Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma
por: Mirizio, Emily, et al.
Publicado: (2020)

Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography
por: Schild, Erik S., et al.
Publicado: (2021)

Correction to: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma
por: Mirizio, Emily, et al.
Publicado: (2021)

Protocol to dissociate healthy and infected murine- and hamster-derived lung tissue for single-cell transcriptome analysis
por: Pennitz, Peter, et al.
Publicado: (2022)

Effects of Cryopreservation and Thawing on Single-Cell Transcriptomes of Human T Cells
por: Lee, Jeong Seok, et al.
Publicado: (2020)

Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons
por: Peitz, Michael, et al.
Publicado: (2020)

Calculi in Kidneys and Ureters
por: Henderson, W. H.
Publicado: (1901)

Acquisition, processing, and single-cell analysis of normal human breast tissues from a biobank
por: Bhat-Nakshatri, Poornima, et al.
Publicado: (2021)

Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq
por: Quintanal-Villalonga, Álvaro, et al.
Publicado: (2022)

Comparison of freshly cultured versus freshly thawed (cryopreserved) mesenchymal stem cells in preclinical in vivo models of inflammation: a protocol for a preclinical systematic review and meta-analysis
por: Dave, Chintan, et al.
Publicado: (2020)

Highly efficient induced pluripotent stem cell reprogramming of cryopreserved lymphoblastoid cell lines
por: Kumar, Satish, et al.
Publicado: (2020)

Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes
por: Maas, Renee G.C., et al.
Publicado: (2021)

A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis
por: Shah, Mahfuzur R., et al.
Publicado: (2021)

Protocol for cryopreservation of red blood cells that controls ice crystal formation by fulvic acid
por: Hu, Jinhao, et al.
Publicado: (2023)

Pancake kidney with cysts and a single ureter
por: da Silva, Renata Mendes, et al.
Publicado: (2016)

Identification and Characterisation of Infiltrating Immune Cells in Malignant Pleural Mesothelioma Using Spatial Transcriptomics
por: Shek, Dmitrii, et al.
Publicado: (2023)

Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes
por: Guldner, Ian H., et al.
Publicado: (2021)

Developmental Anomalies of Kidney and Ureter
por: Murray, H. E., et al.
Publicado: (1941)

Spatial multi-omics sequencing for fixed tissue via DBiT-seq
por: Su, Graham, et al.
Publicado: (2021)

Proteoglycan in porcine aortic tissue after cryopreservation.
por: Shon, Y. H.
Publicado: (1997)

Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
por: Schulze, Thomas T., et al.
Publicado: (2022)

Single-Cell RNA Sequencing with Spatial Transcriptomics of Cancer Tissues
por: Ahmed, Rashid, et al.
Publicado: (2022)

Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
por: Abrecht, Charlotte, et al.
Publicado: (2022)

Single-Cell Transcriptomics of Human Lymph Node Stroma
por: Takeda, Akira, et al.
Publicado: (2020)

Comparison of quartz vials with polypropylene vials for rapid cryopreservation of human ovarian tissue
por: Scheiner, D., et al.
Publicado: (2016)

Protocol for single-nucleus ATAC sequencing and bioinformatic analysis in frozen human brain tissue
por: Shi, Zechuan, et al.
Publicado: (2022)

Protect the Ureters
por: Redan, Jay A., et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_92kus703elebv4qicmo4kp2af4