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Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development
Renal cell carcinoma (RCC) is one of the leading causes of death in men. Messenger ribonucleic acid (mRNA) vaccines may be an attractive means to achieve satisfactory results. Cancer immunotherapy is a promising cancer treatment strategy. However, immunotherapy is not widely used in renal cell carci...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669058/ https://www.ncbi.nlm.nih.gov/pubmed/36405755 http://dx.doi.org/10.3389/fimmu.2022.1037808 |
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author | Chen, Xinglin Zhang, Tongtong Zhai, Xinyu Wan, Zhong Ge, Minyao Liu, Chengzong Tan, Mingyue Xu, Dongliang |
author_facet | Chen, Xinglin Zhang, Tongtong Zhai, Xinyu Wan, Zhong Ge, Minyao Liu, Chengzong Tan, Mingyue Xu, Dongliang |
author_sort | Chen, Xinglin |
collection | PubMed |
description | Renal cell carcinoma (RCC) is one of the leading causes of death in men. Messenger ribonucleic acid (mRNA) vaccines may be an attractive means to achieve satisfactory results. Cancer immunotherapy is a promising cancer treatment strategy. However, immunotherapy is not widely used in renal cell carcinoma, as only a few patients show a positive response. The present study aimed to identify potential antigens associated with renal cell carcinoma to develop an anti-renal cell carcinoma mRNA vaccine. Moreover, the immune subtypes of renal cell carcinoma cells were determined. The Cancer Genome Atlas (TCGA) analysis revealed gene expression profiles and clinical information. Antigen-presenting cells infiltrated the immune system using Tumor Immune Estimation Resource (TIMER) tool (http://timer.cistrome.org/). GDSC (Genomics of Drug Sensitivity in Cancer) database were used to estimate drug sensitivity. The 13 immune-related genes discovery could be targets for immunotherapy in renal cell carcinoma patients, as they were associated with a better prognosis and a higher level of antigen-presenting cells. These immune subtypes have significant relationships with immunological checkpoints, immunogenic cell death regulators, and RCC prognostic variables. Furthermore, DBH-AS1 was identified as a potential antigen for developing an mRNA vaccine. The CCK8 assay demonstrated that the proliferative capacity of 786-O and Caki-1 cells overexpressing DBH-AS1 was higher than in the control group. In addition, transwell assay revealed that 786-O and Caki-1 cells overexpressing DBH-AS1 showed higher invasion capacity compared with control. This study provides a theoretical basis for the development of mRNA vaccines. Our findings suggest that DBH-AS1 could be potential antigens for developing RCC mRNA vaccines. |
format | Online Article Text |
id | pubmed-9669058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96690582022-11-18 Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development Chen, Xinglin Zhang, Tongtong Zhai, Xinyu Wan, Zhong Ge, Minyao Liu, Chengzong Tan, Mingyue Xu, Dongliang Front Immunol Immunology Renal cell carcinoma (RCC) is one of the leading causes of death in men. Messenger ribonucleic acid (mRNA) vaccines may be an attractive means to achieve satisfactory results. Cancer immunotherapy is a promising cancer treatment strategy. However, immunotherapy is not widely used in renal cell carcinoma, as only a few patients show a positive response. The present study aimed to identify potential antigens associated with renal cell carcinoma to develop an anti-renal cell carcinoma mRNA vaccine. Moreover, the immune subtypes of renal cell carcinoma cells were determined. The Cancer Genome Atlas (TCGA) analysis revealed gene expression profiles and clinical information. Antigen-presenting cells infiltrated the immune system using Tumor Immune Estimation Resource (TIMER) tool (http://timer.cistrome.org/). GDSC (Genomics of Drug Sensitivity in Cancer) database were used to estimate drug sensitivity. The 13 immune-related genes discovery could be targets for immunotherapy in renal cell carcinoma patients, as they were associated with a better prognosis and a higher level of antigen-presenting cells. These immune subtypes have significant relationships with immunological checkpoints, immunogenic cell death regulators, and RCC prognostic variables. Furthermore, DBH-AS1 was identified as a potential antigen for developing an mRNA vaccine. The CCK8 assay demonstrated that the proliferative capacity of 786-O and Caki-1 cells overexpressing DBH-AS1 was higher than in the control group. In addition, transwell assay revealed that 786-O and Caki-1 cells overexpressing DBH-AS1 showed higher invasion capacity compared with control. This study provides a theoretical basis for the development of mRNA vaccines. Our findings suggest that DBH-AS1 could be potential antigens for developing RCC mRNA vaccines. Frontiers Media S.A. 2022-11-03 /pmc/articles/PMC9669058/ /pubmed/36405755 http://dx.doi.org/10.3389/fimmu.2022.1037808 Text en Copyright © 2022 Chen, Zhang, Zhai, Wan, Ge, Liu, Tan and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Chen, Xinglin Zhang, Tongtong Zhai, Xinyu Wan, Zhong Ge, Minyao Liu, Chengzong Tan, Mingyue Xu, Dongliang Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
title | Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
title_full | Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
title_fullStr | Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
title_full_unstemmed | Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
title_short | Identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
title_sort | identifying tumor antigens and immune subtypes of renal cell carcinoma for immunotherapy development |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669058/ https://www.ncbi.nlm.nih.gov/pubmed/36405755 http://dx.doi.org/10.3389/fimmu.2022.1037808 |
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