Cargando…

Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese

The study aimed to investigate the effects of honeysuckle extract (HE) on growth performance, serum biochemical indexes, immune organ indexes, gut morphology, and gut microbes in geese. A total of 180 28-day-old Holdobaki geese were randomly divided into three groups. Each group contained 6 replicat...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Guangquan, Wang, Xianze, Liu, Yi, Wang, Cui, Yang, Yunzhou, Gong, Shaoming, Zhu, Lihui, He, Daqian, Wang, Huiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669450/
https://www.ncbi.nlm.nih.gov/pubmed/36406074
http://dx.doi.org/10.3389/fvets.2022.1006318
_version_ 1784832101221138432
author Li, Guangquan
Wang, Xianze
Liu, Yi
Wang, Cui
Yang, Yunzhou
Gong, Shaoming
Zhu, Lihui
He, Daqian
Wang, Huiying
author_facet Li, Guangquan
Wang, Xianze
Liu, Yi
Wang, Cui
Yang, Yunzhou
Gong, Shaoming
Zhu, Lihui
He, Daqian
Wang, Huiying
author_sort Li, Guangquan
collection PubMed
description The study aimed to investigate the effects of honeysuckle extract (HE) on growth performance, serum biochemical indexes, immune organ indexes, gut morphology, and gut microbes in geese. A total of 180 28-day-old Holdobaki geese were randomly divided into three groups. Each group contained 6 replicates (10 geese, with 5 males and 5 females). The BD group was fed the basal diet, the HE1 group was fed the basal diet supplemented with 1 g/kg of HE, and the HE2 group was fed the basal diet supplemented with 2 g/kg of HE. The experiment lasted for 42 days. The results showed that, compared with the BD group, the average daily gain (ADG) of the HE1 and HE2 groups tended to increase (0.05 < P < 0.10), but the average daily feed intake (ADFI) and final body weight (BW) did not differ significantly, and the feed/gain ratio (F/G) was significantly lower (P < 0.01). The bursa index and the thymus index tended to increase (0.05 < P < 0.10), and serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels increased significantly (P < 0.05). In the HE1 and HE2 groups, the crypt depth (CD) in the jejunum tended to decrease (0.05 < P < 0.10), and the villus height/crypt depth ratio (V/C) increased significantly in the jejunum and the ileum (P < 0.05). According to 16sRNA microbial community diversity analysis, Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria were the dominant phyla. The abundance of Firmicutes was significantly decreased (P < 0.01), while that of Bacteroidetes was significantly increased (P < 0.01), in the HE1 and HE2 groups compared with the BD group. Bacteroides barnesiae, Subdoligranulum variabile, Bacteroides plebeius, and Faecalibacterium prausnitzii were the dominant species, and the abundance of B. plebeius and F. prausnitzii was significantly increased (P < 0.05). According to the LEfSe analysis, BD enriched g_Dorea and g_Dehalobacterium; HE1 enriched g_Faecalibacterium, g_Dialister, g_Prevotella, g_Megamonas, g_Phascolarctobacterium, g_Paraprevotella, g_Anaerostipes, g_Staphylococcus, g_Odoribacter, g_Succinivibrio, and g_Sutterella; and HE2 enriched g_Parabacteroides, g_Olsenella, g_human, and g_Rikenella. According to the Spearman correlation analysis, Bacteroides plebeius was positively correlated with final BW, ADG, IgA, IgG, VH (ileum), and V/C (ileum) and was negatively correlated with F/G and CD (ileum); Ruminococcus gnavus was negatively correlated with final BW, ADG, IgA, and IgG. HE supplementation at 1 g/kg improved growth performance, immune performance, gut morphology, and cecal microbes.
format Online
Article
Text
id pubmed-9669450
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96694502022-11-18 Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese Li, Guangquan Wang, Xianze Liu, Yi Wang, Cui Yang, Yunzhou Gong, Shaoming Zhu, Lihui He, Daqian Wang, Huiying Front Vet Sci Veterinary Science The study aimed to investigate the effects of honeysuckle extract (HE) on growth performance, serum biochemical indexes, immune organ indexes, gut morphology, and gut microbes in geese. A total of 180 28-day-old Holdobaki geese were randomly divided into three groups. Each group contained 6 replicates (10 geese, with 5 males and 5 females). The BD group was fed the basal diet, the HE1 group was fed the basal diet supplemented with 1 g/kg of HE, and the HE2 group was fed the basal diet supplemented with 2 g/kg of HE. The experiment lasted for 42 days. The results showed that, compared with the BD group, the average daily gain (ADG) of the HE1 and HE2 groups tended to increase (0.05 < P < 0.10), but the average daily feed intake (ADFI) and final body weight (BW) did not differ significantly, and the feed/gain ratio (F/G) was significantly lower (P < 0.01). The bursa index and the thymus index tended to increase (0.05 < P < 0.10), and serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels increased significantly (P < 0.05). In the HE1 and HE2 groups, the crypt depth (CD) in the jejunum tended to decrease (0.05 < P < 0.10), and the villus height/crypt depth ratio (V/C) increased significantly in the jejunum and the ileum (P < 0.05). According to 16sRNA microbial community diversity analysis, Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria were the dominant phyla. The abundance of Firmicutes was significantly decreased (P < 0.01), while that of Bacteroidetes was significantly increased (P < 0.01), in the HE1 and HE2 groups compared with the BD group. Bacteroides barnesiae, Subdoligranulum variabile, Bacteroides plebeius, and Faecalibacterium prausnitzii were the dominant species, and the abundance of B. plebeius and F. prausnitzii was significantly increased (P < 0.05). According to the LEfSe analysis, BD enriched g_Dorea and g_Dehalobacterium; HE1 enriched g_Faecalibacterium, g_Dialister, g_Prevotella, g_Megamonas, g_Phascolarctobacterium, g_Paraprevotella, g_Anaerostipes, g_Staphylococcus, g_Odoribacter, g_Succinivibrio, and g_Sutterella; and HE2 enriched g_Parabacteroides, g_Olsenella, g_human, and g_Rikenella. According to the Spearman correlation analysis, Bacteroides plebeius was positively correlated with final BW, ADG, IgA, IgG, VH (ileum), and V/C (ileum) and was negatively correlated with F/G and CD (ileum); Ruminococcus gnavus was negatively correlated with final BW, ADG, IgA, and IgG. HE supplementation at 1 g/kg improved growth performance, immune performance, gut morphology, and cecal microbes. Frontiers Media S.A. 2022-11-03 /pmc/articles/PMC9669450/ /pubmed/36406074 http://dx.doi.org/10.3389/fvets.2022.1006318 Text en Copyright © 2022 Li, Wang, Liu, Wang, Yang, Gong, Zhu, He and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Guangquan
Wang, Xianze
Liu, Yi
Wang, Cui
Yang, Yunzhou
Gong, Shaoming
Zhu, Lihui
He, Daqian
Wang, Huiying
Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
title Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
title_full Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
title_fullStr Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
title_full_unstemmed Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
title_short Supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
title_sort supplementation with honeysuckle extract improves growth performance, immune performance, gut morphology, and cecal microbes in geese
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669450/
https://www.ncbi.nlm.nih.gov/pubmed/36406074
http://dx.doi.org/10.3389/fvets.2022.1006318
work_keys_str_mv AT liguangquan supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT wangxianze supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT liuyi supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT wangcui supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT yangyunzhou supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT gongshaoming supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT zhulihui supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT hedaqian supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese
AT wanghuiying supplementationwithhoneysuckleextractimprovesgrowthperformanceimmuneperformancegutmorphologyandcecalmicrobesingeese