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A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells
Pancreatic cancer (PC) is one of the deadliest human malignancies, and exploring the complex molecular mechanisms behind cell death will greatly promote the clinical treatment of PC. Here, we reported a cascading-response fluorescent-imaging probe, Cy-Cys-pH, for the sequential detection of cysteine...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669487/ https://www.ncbi.nlm.nih.gov/pubmed/36406226 http://dx.doi.org/10.3389/fbioe.2022.1062781 |
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author | Qin, Xue Zhang, Shuping Guo, Xiaolu Liu, Xingyue Shen, Xing-Can |
author_facet | Qin, Xue Zhang, Shuping Guo, Xiaolu Liu, Xingyue Shen, Xing-Can |
author_sort | Qin, Xue |
collection | PubMed |
description | Pancreatic cancer (PC) is one of the deadliest human malignancies, and exploring the complex molecular mechanisms behind cell death will greatly promote the clinical treatment of PC. Here, we reported a cascading-response fluorescent-imaging probe, Cy-Cys-pH, for the sequential detection of cysteine (Cys) and pH in pancreatic cancer cells. In the presence of Cys, Cys-mediated cleavage of the acrylate group caused Cy-Cys-pH to be transformed into Cy-Cys-O, which induced intense fluorescence enhancement at 725 nm. Then, Cy-Cys-O was protonated to obtain Cy-Cys-OH and the fluorescence emission shifted to 682 nm, showing a ratiometric pH response. Furthermore, Cy-Cys-pH can monitor the intracellular pH during the therapeutic process with anticancer drugs and evaluated the ability of three anticancer drugs to kill Panc-1 cells, proving that associating Cys and pH is in part an effective anticancer strategy in the treatment of pancreatic cancer. Significantly, Cy-Cys-pH is able to monitor and image pH changes during Cys depletion in real-time, which further reveals the molecular mechanism of Cys-depleted pancreatic cancer cell death, providing a powerful molecular tool for the precise treatment of PC. |
format | Online Article Text |
id | pubmed-9669487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96694872022-11-18 A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells Qin, Xue Zhang, Shuping Guo, Xiaolu Liu, Xingyue Shen, Xing-Can Front Bioeng Biotechnol Bioengineering and Biotechnology Pancreatic cancer (PC) is one of the deadliest human malignancies, and exploring the complex molecular mechanisms behind cell death will greatly promote the clinical treatment of PC. Here, we reported a cascading-response fluorescent-imaging probe, Cy-Cys-pH, for the sequential detection of cysteine (Cys) and pH in pancreatic cancer cells. In the presence of Cys, Cys-mediated cleavage of the acrylate group caused Cy-Cys-pH to be transformed into Cy-Cys-O, which induced intense fluorescence enhancement at 725 nm. Then, Cy-Cys-O was protonated to obtain Cy-Cys-OH and the fluorescence emission shifted to 682 nm, showing a ratiometric pH response. Furthermore, Cy-Cys-pH can monitor the intracellular pH during the therapeutic process with anticancer drugs and evaluated the ability of three anticancer drugs to kill Panc-1 cells, proving that associating Cys and pH is in part an effective anticancer strategy in the treatment of pancreatic cancer. Significantly, Cy-Cys-pH is able to monitor and image pH changes during Cys depletion in real-time, which further reveals the molecular mechanism of Cys-depleted pancreatic cancer cell death, providing a powerful molecular tool for the precise treatment of PC. Frontiers Media S.A. 2022-11-03 /pmc/articles/PMC9669487/ /pubmed/36406226 http://dx.doi.org/10.3389/fbioe.2022.1062781 Text en Copyright © 2022 Qin, Zhang, Guo, Liu and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Qin, Xue Zhang, Shuping Guo, Xiaolu Liu, Xingyue Shen, Xing-Can A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells |
title | A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells |
title_full | A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells |
title_fullStr | A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells |
title_full_unstemmed | A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells |
title_short | A cascading-response fluorescent probe for real-time pH monitoring during cysteine-depletion process in pancreatic cancer cells |
title_sort | cascading-response fluorescent probe for real-time ph monitoring during cysteine-depletion process in pancreatic cancer cells |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669487/ https://www.ncbi.nlm.nih.gov/pubmed/36406226 http://dx.doi.org/10.3389/fbioe.2022.1062781 |
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