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Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran

This study investigated the molecular epidemiology of carbapenem-resistant classic Klebsiella pneumoniae (CR-cKp) and carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) isolates in southwestern Iran. From 2019 to 2021, 136 (88.9%) cKp and 17 (11.1%) hvKp isolates were identified usin...

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Autores principales: Saki, Morteza, Amin, Mansour, Savari, Mohammad, Hashemzadeh, Mohammad, Seyedian, Seyed Saeid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669656/
https://www.ncbi.nlm.nih.gov/pubmed/36406386
http://dx.doi.org/10.3389/fmicb.2022.1029686
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author Saki, Morteza
Amin, Mansour
Savari, Mohammad
Hashemzadeh, Mohammad
Seyedian, Seyed Saeid
author_facet Saki, Morteza
Amin, Mansour
Savari, Mohammad
Hashemzadeh, Mohammad
Seyedian, Seyed Saeid
author_sort Saki, Morteza
collection PubMed
description This study investigated the molecular epidemiology of carbapenem-resistant classic Klebsiella pneumoniae (CR-cKp) and carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) isolates in southwestern Iran. From 2019 to 2021, 136 (88.9%) cKp and 17 (11.1%) hvKp isolates were identified using biochemical tests and polymerase chain reaction (PCR). Antibiotic resistance, beta-lactamases, and clonal relatedness of carbapenem-resistant isolates were investigated using disk diffusion, PCR, and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), respectively. The different markers of hvKp isolates were as follows: string test (35.3%, n = 6/17), magA (11.8%, n = 2/17), rmpA (11.8%, n = 2/17), rmpA2 (52.9%, n = 9/17), iucA (52.9%, n = 9/17), and peg344 (35.3%, n = 6/17). Also, 55.1% (n = 75/136) of cKp and 47.1% (n = 8/17) of hvKp isolates were CR-cKp and CR-hvKp, respectively. All CR-hvKp (100.0%, n = 8) isolates were MDR. Colistin, tetracycline, and tigecycline were the most effective antibiotics. The occurrence of beta-lactamase genes in 75 CR-cKp and 8 CR-hvKp isolates was as follows: bla(NDM) (41.3, 25.0%), bla(IMP) (4.0, 0.0%), bla(VIM) (8.0, 0.0%), bla(GES) (14.7, 25.0%), bla(OXA–48–like) (20.0, 0.0%), bla(CTX–M) (26.7, 12.5%), bla(SHV) (24.0, 12.5%), bla(TEM) (10.7, 0.0%), bla(FOX) (6.7, 0.0%), bla(DHA) (6.7, 0.0%), bla(CMY) (5.3, 0.0%), bla(LAT) (12.0, 0.0%), and bla(ACT) (8.0, 0.0%). ERIC-PCR showed a high diversity among isolates. In this study, the occurrence of MDR CR-hvKp isolates harboring bla(NDM) and bla(GES) was detected for the first time in southwestern Iran. To prevent the spread of CR-hvKp and reduce selection pressure, long-term surveillance and more effective treatment strategies should be implemented.
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spelling pubmed-96696562022-11-18 Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran Saki, Morteza Amin, Mansour Savari, Mohammad Hashemzadeh, Mohammad Seyedian, Seyed Saeid Front Microbiol Microbiology This study investigated the molecular epidemiology of carbapenem-resistant classic Klebsiella pneumoniae (CR-cKp) and carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) isolates in southwestern Iran. From 2019 to 2021, 136 (88.9%) cKp and 17 (11.1%) hvKp isolates were identified using biochemical tests and polymerase chain reaction (PCR). Antibiotic resistance, beta-lactamases, and clonal relatedness of carbapenem-resistant isolates were investigated using disk diffusion, PCR, and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), respectively. The different markers of hvKp isolates were as follows: string test (35.3%, n = 6/17), magA (11.8%, n = 2/17), rmpA (11.8%, n = 2/17), rmpA2 (52.9%, n = 9/17), iucA (52.9%, n = 9/17), and peg344 (35.3%, n = 6/17). Also, 55.1% (n = 75/136) of cKp and 47.1% (n = 8/17) of hvKp isolates were CR-cKp and CR-hvKp, respectively. All CR-hvKp (100.0%, n = 8) isolates were MDR. Colistin, tetracycline, and tigecycline were the most effective antibiotics. The occurrence of beta-lactamase genes in 75 CR-cKp and 8 CR-hvKp isolates was as follows: bla(NDM) (41.3, 25.0%), bla(IMP) (4.0, 0.0%), bla(VIM) (8.0, 0.0%), bla(GES) (14.7, 25.0%), bla(OXA–48–like) (20.0, 0.0%), bla(CTX–M) (26.7, 12.5%), bla(SHV) (24.0, 12.5%), bla(TEM) (10.7, 0.0%), bla(FOX) (6.7, 0.0%), bla(DHA) (6.7, 0.0%), bla(CMY) (5.3, 0.0%), bla(LAT) (12.0, 0.0%), and bla(ACT) (8.0, 0.0%). ERIC-PCR showed a high diversity among isolates. In this study, the occurrence of MDR CR-hvKp isolates harboring bla(NDM) and bla(GES) was detected for the first time in southwestern Iran. To prevent the spread of CR-hvKp and reduce selection pressure, long-term surveillance and more effective treatment strategies should be implemented. Frontiers Media S.A. 2022-11-03 /pmc/articles/PMC9669656/ /pubmed/36406386 http://dx.doi.org/10.3389/fmicb.2022.1029686 Text en Copyright © 2022 Saki, Amin, Savari, Hashemzadeh and Seyedian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Saki, Morteza
Amin, Mansour
Savari, Mohammad
Hashemzadeh, Mohammad
Seyedian, Seyed Saeid
Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran
title Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran
title_full Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran
title_fullStr Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran
title_full_unstemmed Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran
title_short Beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent Klebsiella pneumoniae clinical isolates from southwest of Iran
title_sort beta-lactamase determinants and molecular typing of carbapenem-resistant classic and hypervirulent klebsiella pneumoniae clinical isolates from southwest of iran
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669656/
https://www.ncbi.nlm.nih.gov/pubmed/36406386
http://dx.doi.org/10.3389/fmicb.2022.1029686
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