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EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis
BACKGROUND: EC-18, a synthetic monoacetyldiaglyceride, exhibits protective effects against lung inflammation, allergic asthma, and abdominal sepsis. However, there have been no investigations to determine whether EC-18 has preventive potential in autoimmune diseases, especially rheumatoid arthritis...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9670482/ https://www.ncbi.nlm.nih.gov/pubmed/36397156 http://dx.doi.org/10.1186/s13075-022-02941-4 |
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author | Park, Jin-Sil Yang, Seung Cheon Jeong, Ha Yeon Lee, Seon-Young Ryu, Jun-Geol Choi, Jeong Won Kang, Hye Yeon Kim, Sung-Min Hwang, Sun-Hee Cho, Mi-La Park, Sung-Hwan |
author_facet | Park, Jin-Sil Yang, Seung Cheon Jeong, Ha Yeon Lee, Seon-Young Ryu, Jun-Geol Choi, Jeong Won Kang, Hye Yeon Kim, Sung-Min Hwang, Sun-Hee Cho, Mi-La Park, Sung-Hwan |
author_sort | Park, Jin-Sil |
collection | PubMed |
description | BACKGROUND: EC-18, a synthetic monoacetyldiaglyceride, exhibits protective effects against lung inflammation, allergic asthma, and abdominal sepsis. However, there have been no investigations to determine whether EC-18 has preventive potential in autoimmune diseases, especially rheumatoid arthritis (RA). METHODS: To investigate the efficacy of EC-18 on the development of RA, EC-18 was administered in a collagen-induced arthritis (CIA) murine model and disease severity and the level of inflammatory cytokines in the joint were investigated. The effect of EC-18 on the inflammation-related factors was investigated by flow cytometry, ELISA, western blot, and real-time PCR in splenocytes from mice and in peripheral blood mononuclear cells from healthy and patients with RA. The effect of EC-18 on osteoclastogenesis was investigated. RESULTS: EC-18 effectively reduced the clinical and histological severity of arthritis, similar to Janus kinase inhibitors include tofacitinib and baricitinib, in CIA. Furthermore, EC-18 exhibited a synergistic effect with methotrexate in preventing CIA. Treatment with EC-18 effectively reduced the production of inflammatory cytokines in immune cells and osteoclast differentiation in mice and patients with RA. CONCLUSION: These results suggest that EC-18 may be an effective strategy for RA. |
format | Online Article Text |
id | pubmed-9670482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-96704822022-11-18 EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis Park, Jin-Sil Yang, Seung Cheon Jeong, Ha Yeon Lee, Seon-Young Ryu, Jun-Geol Choi, Jeong Won Kang, Hye Yeon Kim, Sung-Min Hwang, Sun-Hee Cho, Mi-La Park, Sung-Hwan Arthritis Res Ther Research BACKGROUND: EC-18, a synthetic monoacetyldiaglyceride, exhibits protective effects against lung inflammation, allergic asthma, and abdominal sepsis. However, there have been no investigations to determine whether EC-18 has preventive potential in autoimmune diseases, especially rheumatoid arthritis (RA). METHODS: To investigate the efficacy of EC-18 on the development of RA, EC-18 was administered in a collagen-induced arthritis (CIA) murine model and disease severity and the level of inflammatory cytokines in the joint were investigated. The effect of EC-18 on the inflammation-related factors was investigated by flow cytometry, ELISA, western blot, and real-time PCR in splenocytes from mice and in peripheral blood mononuclear cells from healthy and patients with RA. The effect of EC-18 on osteoclastogenesis was investigated. RESULTS: EC-18 effectively reduced the clinical and histological severity of arthritis, similar to Janus kinase inhibitors include tofacitinib and baricitinib, in CIA. Furthermore, EC-18 exhibited a synergistic effect with methotrexate in preventing CIA. Treatment with EC-18 effectively reduced the production of inflammatory cytokines in immune cells and osteoclast differentiation in mice and patients with RA. CONCLUSION: These results suggest that EC-18 may be an effective strategy for RA. BioMed Central 2022-11-17 2022 /pmc/articles/PMC9670482/ /pubmed/36397156 http://dx.doi.org/10.1186/s13075-022-02941-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Park, Jin-Sil Yang, Seung Cheon Jeong, Ha Yeon Lee, Seon-Young Ryu, Jun-Geol Choi, Jeong Won Kang, Hye Yeon Kim, Sung-Min Hwang, Sun-Hee Cho, Mi-La Park, Sung-Hwan EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
title | EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
title_full | EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
title_fullStr | EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
title_full_unstemmed | EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
title_short | EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
title_sort | ec-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9670482/ https://www.ncbi.nlm.nih.gov/pubmed/36397156 http://dx.doi.org/10.1186/s13075-022-02941-4 |
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